全文获取类型
收费全文 | 189篇 |
免费 | 1篇 |
国内免费 | 6篇 |
出版年
2020年 | 1篇 |
2018年 | 2篇 |
2016年 | 1篇 |
2015年 | 1篇 |
2014年 | 7篇 |
2013年 | 14篇 |
2012年 | 3篇 |
2011年 | 10篇 |
2010年 | 5篇 |
2009年 | 6篇 |
2008年 | 4篇 |
2007年 | 8篇 |
2006年 | 17篇 |
2005年 | 11篇 |
2004年 | 15篇 |
2003年 | 8篇 |
2002年 | 13篇 |
2001年 | 6篇 |
2000年 | 7篇 |
1999年 | 11篇 |
1998年 | 7篇 |
1997年 | 3篇 |
1996年 | 3篇 |
1995年 | 5篇 |
1994年 | 1篇 |
1993年 | 5篇 |
1992年 | 4篇 |
1991年 | 2篇 |
1990年 | 1篇 |
1987年 | 2篇 |
1985年 | 3篇 |
1984年 | 1篇 |
1983年 | 1篇 |
1982年 | 2篇 |
1981年 | 1篇 |
1980年 | 2篇 |
1979年 | 1篇 |
1975年 | 1篇 |
1973年 | 1篇 |
排序方式: 共有196条查询结果,搜索用时 593 毫秒
191.
G.Parenti Castelli A. Baracca R. Fato A. Rabbi 《Biochemical and biophysical research communications》1983,111(2):366-372
The temperature dependence of the intrinsic tryptophan fluorescence in either bovine heart submitochondrial particles or oligomycin-sensitive ATPase isolated therefrom shows a discontinuity at near 25°C, which coincides with the temperature where a break in the Arrhenius plot of ATPase activity is found. Addition of n-butanol to submitochondrial particles induces a decrease of tryptophan fluorescence in the whole temperature range. The discontinuity is interpreted as a temperature-dependent structural change and related to a viscosity-induced phase separation of the intrinsic mitochondrial proteins. 相似文献
192.
193.
194.
Molecular characterisation of the inactive allele of the gene Glu-A1 and the development of a set of AS-PCR markers for HMW glutenins of wheat 总被引:3,自引:0,他引:3
A. De Bustos P. Rubio N. Jouve 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2000,100(7):1085-1094
The present work reports new PCR markers that amplify the complete coding sequence of the specific alleles of the high molecular
weight (HMW) glutenin genes. A set of AS-PCR molecular markers was designed which use primers from nucleotide sequences of
the Glu-A1 and Glu-D1 genes, making use of the minor diffeences between the sequences of the x1, x2* of Glu-A1, and the x5 and y10 of Glu-D1. These primers were able to distinguish between x2* and the x1 or xNull of Glu-A1. Also x5 was distinguishable from x2, and y10 from y12. The primers amplified the complete coding regions and corresponded to the upstream and downstream flanking positions of Glu-A1 and Glu-D1. Primers designed to amplify the Glu-A1 gene amplified a single product when used with genomic DNA of common wheats and the xNull allele of this gene. This work also describes the cloning and characterisation of the nucleotide sequence of this allele.
It possesses the same general structure as x2* and x1 (previously determined) and differs from these alleles in the extension of the coding sequence for a presumptive mature protein
with only 384 residues. This is due to the presence of a stop codon (TAA) 1215-bp downstream from the start codon. A further
stop codon (TAG), 2280-bp downstream from the starting codon is also found. The open reading frame of xNull and x1 alleles has the same size in bp. Both are larger than x2* which shows two small deletions. The reduced size of the presumptive mature protein encoded by xNull could explain the negative effect of this allele on grain quality.
Received: 16 May 1999 / Accepted: 16 September 1999 相似文献
195.
D.C. Clark S.R. Martin P.M. Bayley 《Biochemical and biophysical research communications》1980,97(2):628-634
The near-UV circular dichroism properties of tubulin dimer have been measured for different preparative methods. Tubulin dimer was obtained from assembly compenent microtubule protein by gel filtration, or phosphocellulose ion-exchange chromatography in the presence of magnesium. Tubulin dimer prepared by the protocol of Weisenberg R.C. and Timasheff, S.N. (1970) Biochemistry 9, 4110–4116, was found to be markedly different due to some apparently irreversible change in conformation. We conclude that the removal of microtubule-associated proteins by phosphocellulose ion-exchange chromatography in the presence of magnesium can be performed without affecting the conformation of native tubulin dimer as judged by near-UV circular dichroism. 相似文献
196.
The structure of a small collagenous fragment isolated from chicken hyaline cartilage 总被引:3,自引:0,他引:3
In previous experiments, two collagenous fragments were isolated from pepsin digests of chicken hyaline cartilage and called the high molecular weight, (HMW) and low molecular weight (LMW) fractions [3]. In the present experiments, the chains of LMW were isolated after denaturation and subsequent reduction and alkylation of interchain disulfide bridges and were further fractionated by carboxymethyl-cellulose chromatography. Four peaks were resolved during chromatography and were designated LMW 1, 2A, 2B, and 3. Amino acid analyses and peptide mapping after cleavage with trypsin, V8 protease, and cyanogen bromide showed that three genetically distinct chains must be present in LMW. Fractions 2A and 2B were very similar, but not identical, in structure. LMW 1, 2A plus 2B, and 3 were consistently isolated in approximately equal proportions, suggesting that the probable chain organization of LMW is [1][2A + 2B][3]. This suggestion was supported further by experiments that attempted to fractionate LMW by carboxymethyl-cellulose chromatography after denaturation but without reduction and alkylation of interchain disulfide bridges. No fractionation of LMW was achieved, the single peak subsequently being shown to contain LMW 1, 2A plus 2B, and 3. 相似文献