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91.
92.
Claudia Ordeanu Diana Cristina Pop Radu Badea Csaba Csutak Nicolae Todor Calin Ordeanu Reka Kerekes Ovidiu Coza Viorica Nagy Patriciu Achimas-Cadariu Alexandru Irimie 《Reports of Practical Oncology and Radiotherapy》2015,20(3):223-230
Objective
The aim of study was to analyze the accuracy of TRUS (transrectal ultrasound) vs. MRI (magnetic resonance imaging) and clinical gynecological examination estimation in the evaluation of tumor dimensions.Methods
The patients inclusion criterion included primarily pathologically squamous cell carcinoma, but excluded were patients who had not undergone BT (brachytherapy) and treated with palliative intent. We offer two types of treatment for locally advanced cervical cancer: (a) radiochemotherapy followed by surgery and (b) exclusive radiochemotherapy. Imaging tests follow the presence of tumor and tumor size (width and thickness). Each examination was performed by a different physician who had no knowledge of the others’ findings. All patients underwent MRI prior to EBRT (external beam radiation therapy) while 18 of them also at the time of the first brachytherapy application. For the analysis we used the r-Pearson correlation coefficient.Results
In 2013, 26 patients with cervical cancer were included. A total of 44 gynecological examinations were performed, 44 MRIs and 18 TRUSs. For the comparisons prior to EBRT the correlation coefficient between TRUS vs. MRI was r = 0.79 for AP and r = 0.83 for LL, for GYN vs. MRI was r = 0.6 for AP and r = 0.75 for LL. Prior to BT for GYN vs. MRI, r values were 0.60 and 0.63 for AP and LL, respectively; for GYN vs. TRUS, r values were 0.56 and 0.78 for AP and LL, respectively.Conclusions
A high correlation between the three examinations was obtained. As such, TRUS can be considered a suitable method in the evaluation of tumor dimensions. 相似文献93.
目的:了解舒芬太尼联合昂丹司琼静脉术后镇痛应用妇科手术的临床效果。方法:对我院2012年3月至2014年3月收治的全麻术后患者进行随机抽样,选取80例患者随机分成两组,对照组予以1.0 mg芬太尼联合8 mg昂丹司琼镇痛,实验组予以100μg舒芬太尼联合8 mg昂丹司琼镇痛。观察并比较两组患者的镇痛效果及不良反应的发生状况。结果:对比恶心、呕吐等临床症状,实验组患者的临床情况明显优于对照组,经统计学分析,差异具有统计学意义(P0.05),且镇静评分、疼痛评分亦优于对照组,经统计学分析,差异具有统计学意义(P0.05)。结论:术后持续静脉予以舒芬太尼联合昂丹司琼,能降低术后恶心、呕吐发生率,减轻患者疼痛程度,提升患者临床治疗效果。 相似文献
94.
Introduction
Type B lactic acidosis represents a rare and often lethal complication of haematological malignancy. Here, we present a patient who developed a type B lactic acidosis presumably due to a concurrent chronic myelomonocytic leukaemia. Upon swift initiation of cytoreductive chemotherapy (doxorubicin), the lactic acidosis was rapidly brought under control. This case adds to the literature reporting other haematological malignancies that can cause a type B lactic acidosis and its successful treatment.Case presentation
We report the case of a 77-year-old Caucasian man brought to our Accident and Emergency department following an unwitnessed collapse; he was found surrounded by coffee-ground vomit. Although haemodynamically stable on admission, he rapidly deteriorated as his lactic acid rose. An initial arterial blood gas revealed a pH of 7.27 and lactate of 18mmol/L (peaking at 21mmol/L).Conclusions
A high degree of clinical suspicion for haematological malignancy should be held when presented with a patient with lactic acidosis in clinical practice, even without evidence of poor oxygenation or another cause. Treatment with emergency chemotherapy, in lieu of a definitive diagnosis, was rapidly successful at lowering lactate levels within 8 hours. This may suggest a causal and perhaps direct relationship between lactic acid production and the presence of leukemic cells. Veno-venous haemofiltration had no apparent effect on reducing the lactic acidosis and therefore its benefit is questioned in this setting, especially at the cost of delaying chemotherapy. In the face of a life-threatening lactic acidosis, pragmatic clinical judgement alone may justify the rapid initiation of chemotherapy. 相似文献95.
96.
Yan Cheng Fumou Sun Xing Cui Siegfried Janz 《Asia-Pacific Journal of Blood Types and Genes》2020,4(1):9-18
Genetic myeloma risk research relied on genome-wide association studies to identify 24 common but lowimpact germline predisposition alleles that account for an estimated one eighth of the heritable myeloma risk in
Caucasians. Next-generation sequencing, particularly whole-exome sequencing, uncovered a handful of rare but
high-impact myeloma risk loci that convey intriguing clues about etiology. The recent discovery of NCOA1 as a myeloma susceptibility gene in Han Chinese has set the stage for the more complete elucidation of the genetic myeloma risk across ethnic barriers. Validating individual myeloma risk loci at the functional level and integrating predisposition genes in genetic networks and biological pathways are important research tasks going forward. Candidate pathways that are currently emerging include plasma cell development, autophagy, telomere maintenance, and cell cycle regulation. An outstanding knowledge gap in the area of gene-environment interaction concerns the possibility that tumor-promoting effects of myeloma susceptibility alleles depend on specific environmental or occupational exposures. An implicit promise of myeloma risk research is the detection of new molecular targets for myeloma treatments and preventions. A related outcome is new biomarkers for patient stratification, prognostication, and development of individualized treatment plans. 相似文献
97.
A bioluminescent HL‐60 cell line to assay anti‐leukaemia therapeutics under physiological conditions
Maria P. Isaza Jennie T. Chau Amy Le Nataliya V. Balashova Jigna K. Patel Erica Salerno Juan A. Crosby Anna O'Connor Scott C. Kachlany 《Luminescence》2008,23(1):17-21
Screens for compounds and proteins with anti‐cancer activity employ viability assays using relevant cancer cell lines. For leukaemia studies, the human leukaemia cell line, HL‐60, is often used as a model system. To facilitate the discovery and investigation of anti‐leukaemia therapeutics under physiological conditions, we have engineered HL‐60 cells that stably express firefly luciferase and produce light that can be detected using an in vivo imaging system (IVIS). Bioluminescent HL‐60luc cells could be rapidly detected in whole blood with a sensitivity of approximately 1000 viable cells/200 µl blood. Treatment of HL‐60luc cells with the drug chlorambucil revealed that the bioluminescent viability assay is able to detect cell death earlier than the Trypan blue dye exclusion assay. HL‐60luc cells administered intraperitoneally (i.p.) or intravenously (i.v.) were visualized in living mice. The rapidity and ease of detecting HL‐60luc cells in biological fluid indicates that this cell line could be used in high‐throughput screens for the identification of drugs with anti‐leukaemia activity under physiological conditions. Copyright © 2007 John Wiley & Sons, Ltd. 相似文献
98.
Ming‐Zhong Sun Shuqing Liu Jianwu Tang Zhiqiang Wang Xiaolin Gong Chengrong Sun Frederic Greenaway 《Proteomics》2009,9(12):3285-3302
Lymph node metastasis (LNM) is recognized as an important factor involved in the tumor malignancy progression. Our previous study has indicated that the hepatocarcinoma cell line with 75% of LNM (Hca‐F)‐cell‐induced neoplasia and the hepatocarcinoma cell line with 25% of LNM‐induced neoplasia are accompanied with high (75%) and low (25%) incidences of LNM. In the current study, 62 and 54 protein spots were observed up‐regulated and down‐regulated in Hca‐F cell relative to the hepatocarcinoma cell line with 25% of LNM by 2‐D DIGE. Totally, 113 unique proteins were identified by HPLC‐nano ESI‐MS/MS analysis. The expression levels of Annexin A7, Ulch3, and ER protein 29 were validated by Western blotting analyses. The abnormally regulated proteins were categorized and annotated by protein analysis through evolutionary relationships analysis with the aid of the database for annotation, visualization and integrated discovery tool. Seventeen gene candidates concordantly expressed both at mRNA and protein levels. By making a challenge, we detected expression levels of Annexin A7 in primary gastric cancer (GC) and primary GC cancer tissues with LNMs by immunohistochemisty. Higher ratio of positive and strong expressions Annexin A7 in GC might correlate with the tumor progression. The repression of Annexin A7 inhibits the mobility and invasion abilities of Hca‐F cell, increases the apoptosis rate of Hca‐F cell. Current study narrows and provides certain specific protein candidates potentially playing important roles in LNM‐associated cancers. 相似文献
99.
Jin-ichi Inokuchi Kazuya Kabayama Satoshi Uemura Yasuyuki Igarashi 《Glycoconjugate journal》2003,20(3):169-178
To elucidate the biological significance of the lactosylceramide (LacCer) branching in glycosphingolipid (GSL) biosynthesis,
we established ganglioside GM3- and lactosylsulfatide SM3-reconstituted cells by introducing the GM3 synthase gene and the
sulfotransferase gene, respectively. In SM3-expressing cells, the reduction of β1 integrin mRNA expression, the reduced adhesivity
to fibronectin and laminin, and the suppression of anchorage-independent growth (tumorigenic potential) were observed. On
the other hand, in GM3-expressing cells, anchorage-independent growth was promoted and the expression of PDGFα receptor mRNA
was specifically reduced. Interestingly enough, no change in anchorage-dependent growth was observed in these cells, and tumorigenic
signals were controlled selectively in both positive and negative directions. Thus, the spatio-temporal, gene expression control
mechanism by individual GSL molecules accumulating in the cell membrane microdomain (raft) has been proven. Published in 2004.
This revised version was published online in August 2006 with corrections to the Cover Date. 相似文献
100.
Inactivation of glyceraldehyde-3-phosphate dehydrogenase of human malignant cells by methylglyoxal 总被引:1,自引:0,他引:1
The effect of methylglyoxal on the activity of glyceraldehyde-3-phosphate dehydrogenase (GA3PD) of several normal human tissues and benign and malignant tumors has been tested. Methylglyoxal inactivated GA3PD of all the malignant cells (47 samples) and the degree of inactivation was in the range of 25-90%, but it had no inhibitory effect on this enzyme from several normal cells (24 samples) and benign tumors (13 samples). When the effect of methylglyoxal on other two dehydrogenases namely glucose 6-phosphate dehydrogenase (G6PD) and L-lactic dehydrogenase (LDH) of similar cells was tested as controls it has been observed that methylglyoxal has some inactivating effect on G6PD of all the normal, benign and malignant samples tested, whereas, LDH remained completely unaffected. These studies indicate that the inactivating effect of methylglyoxal on GA3PD specifically of the malignant cells may be a common feature of all the malignant cells, and this phenomenon can be used as a simple and rapid device for the detection of malignancy. 相似文献