Interaction of Press and Pulse Disturbance on Crayfish Populations: Flood Impacts in Pasture and Forest Streams

Interactions between press and pulse disturbances can significantly impact benthic macroinvertebrate (crayfish) populations. Press disturbances such as land-use change can make crayfish more vulnerable to the detrimental effects of pulse disturbances, by changing the habitats available to crayfish. The impact of a pulse disturbance, a major flood (1 in 28 years return period), on crayfish (Paranephrops planifrons) was significantly greater in pasture than native forest streams. Population densities of crayfish in three forest and three pasture streams had been measured seasonally for 2 years prior to the flood and many crayfish had been marked with individually identifiable tags. Low numbers of marked crayfish were recaptured after the flood in the forest streams, but no marked crayfish were identified in any pasture stream. Crayfish densities in one pasture stream declined from an average of 5 m^–2 prior to the flood to <1 m^–2 soon after the flood and it took 3 years for the population to show evidence of recovery. Macrophytes and cobbles, the dominant habitats of crayfish in pasture streams, did not appear to provide stable refugia during the flood. Habitat stability was linked to the riparian zone in forest streams where undercut banks, tree roots, and pools were important habitats for crayfish. Frequent pulse disturbance could affect population persistence of refugia-dependent species when the pressure of land-use change affects the stability of habitats, but this may only be evident over long time scales.     

Manipulation of environment to facilitate use of nematodes in biocontrol of insects

The pre- and postparasitic phases of entomophilic nematodes are exposed to different microenvironments which should be changed in order to facilitate the use of nematodes as biological control agents of insects. The mixing of “thick water” with nematodes prior to spraying on exposed surfaces retards nematode desiccation and so increases the mortality of forest and agricultural insect pests. Aquatic larvae of Simulium vittatum and of Culiseta inornata feed fastest at 20 and 25 C, respectively, and so engulf more infective larvae of Neoaplectana carpocapsae (DD-136). When exposed to N. carpocapsae (DD-136) on potato foliage, the percentage of encapsulated nematodes in the haemocoel of adult Leptinotarsa decemlineata was less than in the third stage nymphs. The percentage of unencapsulated, dead nematodes was greatest in the nymphal stages. The value of baits and phagostimulants to increase the insects' oral uptake of infective nematodes and of the manipulation of the insect's physiological processes so as to modify its susceptibility to nematodes is discussed.     

Assessment of intra-fraction motion during automated linac-based SRS treatment delivery with an open face mask system

Purpose/objectiveTo evaluate intra-fraction target shift during automated mono-isocentric linac-based stereotactic radiosurgery with open-face mask system and optical real-time tracking.Materials/methodsNinety-five patients were treated using automated linac-based stereotactic radiosurgery in 1–5 fractions with single isocenter for a total of 195 fractions. During treatment, patient positioning was tracked real-time with optical surface guidance and immobilized with a rigid open-face mask. Patients were re-positioned if optical surface guidance error exceeded 1 mm magnitude or 1°. Translational and rotational intra-fractional changes were determined by post-treatment CBCT matched to the planning CT. Target specific error was calculated by translation and rotation matrices applied to isocenter and target spatial coordinates.ResultsFor 132 fractions with isocenter within a single target, the median shift magnitude was 0.40 mm with a maximum shift of 1.17 mm. A total of 398 targets treated for plans having multiple or single targets that lied outside isocenter, resulted in a median shift magnitude of 0.46 mm, with median translational shifts of 0.20 mm and 0.20° rotational shifts. A 1 mm PTV margin was insufficient in 18% of targets at a distance greater than 6 cm away from isocenter, but sufficient for 96% of targets within 6 cm.ConclusionsThe findings of this study support 1 mm PTV expansion due to intra-fraction motion to ensure target coverage for plans with isocenter placement less than 6 cm away from the targets.     

A novel acoustic evaluation system of scratching in mouse dermatitis: rapid and specific detection of invisibly rapid scratch in an atopic dermatitis model mouse

Scratching is an essential and a skin specific behavior induced by itching, which is a common symptom of atopic dermatitis (AD) and other types of dermatitis. Itching sensation and scratching are closely associated and thus scratching times are currently used for evaluating itching in animal models. However, objective measuring systems of scratching to assess the grade of dermatitis and the effectiveness of anti-pruritic drugs in animal dermatitis models are lacking. To investigate a quantitative evaluation system for itching, we have developed a novel acoustic scratching counting system and compared its accuracy with time-consuming slow-motion video recording system. We have also objectively evaluated the efficacy of an antihistamine using this novel system. Scratching behavior of an AD model mouse (K14 driven IL-18 transgenic mouse) was recorded visually and acoustically. Specific scratching sound produced by mice was recorded and counted using a software we have developed, and the results obtained using our acoustic system were not statistically different from data obtained using slow motion video system. Surprisingly, mice scratched more than 10 times/second, which was invisibly rapid motion and revealed inaccuracy of conventional hand counting system. Results were identical to that of measured by 10 times time costing slow-motion video analysis. The antihistamine is clearly effective for suppression of scratching as demonstrated using this objective and accurate method. This novel motion analysis system will open a window for physiological and pathological analysis for animal models and development of anti-pruritic drugs.     

The influence of compressive gear on maximal load lifted in competitive powerlifting

The competition in powerlifting has been divided into two divisions, with gear equipment (EQ) and without gear equipment (RAW). When competing in the EQ division, additional supportive gear can be worn by the athletes, while in the RAW division such gear is not allowed. The aim of the study was to compare the results of the RAW and EQ powerlifting divisions based on the results of world championships and current world records. One-hundred and twenty powerlifters (63 men, 57 women) were included to the analysis. Post hoc analysis for the results of men’s world championships indicated significantly higher results of the barbell squat (SQ; p < 0.001; ES = 1.31), bench press (BP; p < 0.001; ES = 1.27) and deadlift (DL; p < 0.001; ES = 0.37) for EQ compared to the RAW division. Post hoc analysis for the results of women’s world championships indicated significantly higher results of the SQ (p < 0.001; ES = 1.31), BP (p < 0.001; ES = 1.13) and DL (p < 0.001; ES = 0.71) for the EQ compared to the RAW division. Post hoc analysis for men’s world record indicated significantly higher results in the SQ (p < 0.001; ES = 1.32) and BP (p < 0.001; ES = 1.24) for the EQ compared to the RAW division. Furthermore, there were no significant differences in the results of world records in the DL (p = 0.901; ES = 0.26) between the EQ and RAW divisions. Post hoc analysis for women’s world records indicated significantly higher results in the SQ (p < 0.001; ES = 1.22) and BP (p < 0.001; ES = 1.99) for the EQ compared to RAW division. The main finding of the study was that supportive gear increases maximal load lifted during powerlifting competition.     

Effects of rapeseed‐press cake glucosinolates and iodine on the performance,the thyroid gland and the liver vitamin a status of pigs

Rapeseed press cake (per kg DM 181 g EE, 341 g CP and 23.3 mmol glucosinolates) was tested in a long‐term experiment with a total of sixty pigs (live weight range 24 to 104 kg). The 3×2 factorial design consisted of three rapeseed press cake levels (no rapeseed press cake ‐ control, 75g or 150g rapeseed press cake per kg diet) each with two iodine dosages (125 or 250 μg supplementary iodine per kg diet). Reduced feed intake and depressed weight gain were found in groups receiving 150 g rapeseed press cake per kg diet, which correspond to 3.2mmol glucosinolates per kg diet. At an inclusion level of 75 g rapeseed‐press cake per kg diet no differences in feed intake and growth intensity were recorded in comparison to the rape feed free control. The rapeseed‐press cake diet increased the weight of thyroid gland and liver and decreased the serum thyroxine (T₄) concentration. Higher iodine dosage increased the serum T₄ concentration of pigs receiving 75 g rapeseed press cake per kg diet ( = 1.6 mmol glucosinolates per kg diet) to the level of the control group and retarded the enlargement of the thyroid gland. Intake of rapeseed products lowered the iodine content of the thyroid gland, however, there was no significant difference between groups given 1.6 and 3.2 mmol glucosinolates per kg diet. The vitamin A content of the whole liver and the vitamin A serum concentration were not influenced by the diets tested. However, rapeseed press cake and the glucosinolates, respectively, decreased the vitamin A concentration per gram liver due to the organ enlargement and the resulting dilution effect.