三种胚胎移植技术方法比较

以近交系FVB、DBA／2小鼠作为供体胚小鼠,封闭群CD-1小鼠作为受体小鼠,对实验小鼠胚胎移植的三种方法进行比较,即：对小鼠受精卵1细胞期胚(或2细胞期胚)经由受体小鼠输卵管口移植、经由受体小鼠输卵管壁移植及对小鼠进行子宫移植(8细胞期胚)的方法进行了实验研究,并对各方法适用于使用的动物对象、实验要求、产仔率、优势与不足等做了进一步探讨,为今后的实验动物提供参考依据。结果表明,输卵管壁移植优于输卵管伞口移植和子宫移植。     

Metabolomic analysis of oviduct fluid on day 3 post-estrus in Holstein heifers

The metabolites in the oviduct fluid (OF) of both oviducts were analyzed by proton nuclear magnetic resonance (¹H-NMR) in Holstein heifers on day 3 after synchronized estrus. Twenty-six metabolites were quantified, among which lactate, glycine and myoinositol were the most abundant. Six metabolites including glycine and myoinositol varied in amount according to the proximity to the corpus luteum. Glucose and histidine were among the most variable metabolites among heifers while threonine and lactate were among the most stable ones, suggesting different mechanisms of homeostasis in the OF.     

耳蜗电图慢波电位

采用同极双道同步记录法对比观察了豚鼠耳蜗电图慢波电位和快波电位的波形;测量了慢波电位的潜伏期、波幅和阈值;并与快波电位的阈值进行了比较。结果表明,慢波电位不但对高频声音反应好,而且对低频声音反应也很好,反应闽都在OdBnHL以下,因而弥补了快波电位对低频声音反应闽值高的缺陷,解决了耳蜗电图低频检测的难题。作者认为,慢波电位主要来源于听神经动作电位的慢成分,其次是听觉脑干诸核团的慢成分。慢波电位是反映频率和强度特性的理想指标,在科研及临床实践中应用将是很有前途的。     

Synergy between IL-8 and GM-CSF in reproductive tract epithelial cell secretions promotes enhanced neutrophil chemotaxis

Neutrophils occur in tissues of the female reproductive tract (FRT) under non-infected conditions. These cells generally enter tissues under the influence of chemoattractants called chemokines. Primary epithelial cells (EC) from FRT were a potent source of chemokines, IL-8 being the chief neutrophil chemoattractant secreted. Blocking with neutralizing anti-IL-8 showed that IL-8 did not account for all of the chemoattraction observed. A mixture of 25 ng/mL rIL-8 and 1 ng/mL rGM-CSF mediated 2.7-fold more chemotaxis than that expected if the two agents were additive. We then found that GM-CSF was produced by EC in amounts that synergised strongly with IL-8 to enhance chemotaxis. Treatment of uterine EC conditioned medium with saturating doses of anti-IL-8 plus anti-GM-CSF antibodies produced an 84% inhibition of chemotaxis. These findings demonstrate that the majority of neutrophil chemoattractant activity produced by FRT EC results from the synergistic effects of IL-8 and GM-CSF.     

解脲支原体对兔输卵管黏膜上皮细胞致病性研究

目的：探讨解脲支原体(UU)对兔输卵管黏膜上皮细胞的粘附及其致病性。方法：采用雌性大白兔作为实验对象,用UU感染兔输卵管黏膜上皮细胞,经光学和电子显微镜观察UU对其粘附性和致病性。结果：UU感染后,兔输卵管管腔有较多渗出物,呈淡红染匀质状,黏膜层的上皮细胞轻度肿胀,黏膜下层有炎症细胞(以淋巴细胞为主)呈灶性浸润;在电镜下,UU粘附于输卵管黏膜上皮细胞上,同时上皮细胞游离面的纤毛互相粘连、倒状,纤毛细胞核膜欠完整．胞核染色质呈凝块状．胞浆内可见大量大小不等的空泡,无纤毛细胞顶面参差不齐,可见伪足样突出．微绒毛减少．胞浆内近细胞顶部有少量分泌颗粒和空泡变性,细胞间可见相嵌连接。结论：UU到达兔输卵管后可粘附于输卵管黏膜上皮细胞上并导致损伤。     

Use of MALDI-TOF mass spectrometry to explore the peptidome and proteome of in-vitro produced bovine embryos pre-exposed to oviduct fluid

In order to identify oviduct fluid (OF) peptides and proteins possibly uptaken by developing embryos, in-vitro produced bovine embryos exposed or not to OF were individually analyzed by MALDI-TOF mass spectrometry. Overall, 11 masses were overabundant in OF-treated embryos compared to controls, among which one at 8.9 kDa annotated as immediate early response 3-interacting protein 1 or a peptide of transitional endoplasmic reticulum ATPase met the criteria of an OF embryo-interacting protein or peptide.