全文获取类型
收费全文 | 159篇 |
免费 | 8篇 |
国内免费 | 3篇 |
专业分类
170篇 |
出版年
2024年 | 1篇 |
2023年 | 4篇 |
2022年 | 3篇 |
2021年 | 5篇 |
2020年 | 10篇 |
2019年 | 12篇 |
2018年 | 2篇 |
2017年 | 4篇 |
2016年 | 6篇 |
2015年 | 2篇 |
2014年 | 1篇 |
2013年 | 7篇 |
2012年 | 2篇 |
2011年 | 4篇 |
2010年 | 2篇 |
2009年 | 8篇 |
2008年 | 3篇 |
2007年 | 6篇 |
2006年 | 4篇 |
2005年 | 6篇 |
2004年 | 3篇 |
2003年 | 3篇 |
2002年 | 6篇 |
2001年 | 7篇 |
2000年 | 2篇 |
1999年 | 7篇 |
1998年 | 2篇 |
1997年 | 2篇 |
1996年 | 4篇 |
1995年 | 3篇 |
1994年 | 3篇 |
1993年 | 2篇 |
1992年 | 5篇 |
1991年 | 6篇 |
1990年 | 4篇 |
1988年 | 2篇 |
1987年 | 1篇 |
1986年 | 2篇 |
1985年 | 3篇 |
1984年 | 5篇 |
1982年 | 2篇 |
1981年 | 2篇 |
1979年 | 1篇 |
1978年 | 1篇 |
排序方式: 共有170条查询结果,搜索用时 15 毫秒
161.
Jeff A. Boyd Clifford A. Rinehart Jr. Leslie A. Walton Gene P. Siegal David G. Kaufman 《In vitro cellular & developmental biology. Plant》1990,26(7):701-708
Summary Two new lines of human endometrial carcinoma (HEC) cells, one from an adenocarcinoma and one from a highly metastatic serous
papillary carcinoma, were established in culture. Structural and morphologic properties of these cells at early passage were
compared with those of cultured normal human endometrial epithelial (NHEE) cells. For these studies, cells were grown on a
conventional plastic surface or on an extracellular matrix substrate (Matrigel), and examined by transmission electron microscopy
and immunofluorescent light microscopy. The HEC cells appeared morphologically similar on plastic and Matrigel, whereas the
NHEE cells showed significantly greater epithelial morphologic differentiation on Matrigel than on plastic. On extracellular
matrix, the morphologic differences observed between HEC cells and NHEE cells were primarily of an architectural nature, which
may be in part explained by differences between NHEE and HEC cells in the arrangement of actin microfilaments and cytokeratin
intermediate filaments. Furthermore, HEC cells displayed extensive networks of vimentin intermediate filaments, which were
absent from the NHEE cells. These observations support the hypothesis that architectural deregulation is a prominent feature
of endometrial carcinoma, and that cytoskeletal alterations may uncouple HEC cell ultrastructural morphology from the influence
of extracellular matrix.
This research was supported by research grants CA31733, CA45727, and ES07017, from the National Institutes of Health, Bethesda,
MD. G. P. S. is a Jefferson Pilot Fellow in Academic Medicine. A preliminary account of this work was presented at the 1988
U.S.-Canadian Academy of Pathology Annual Meeting (Lab. Inves. 58:12a, 1988). 相似文献
162.
163.
Hai-Yan Lin Heng Zhang Qing Yang Hong-Xing Wang Hong-Mei Wang Karl X Chai Li-Mei Chen Cheng Zhu 《The journal of histochemistry and cytochemistry》2006,54(10):1139-1147
Serine proteases have been documented to play key roles in uterine matrix turnover and trophoblastic invasion during implantation. Roles of prostasin serine protease in these processes, however, are currently unclear. The present study was first conducted to investigate the colocalization of prostasin and its cognate serpin, protease nexin-1 (PN-1), in rhesus monkey endometrium and placenta on days 12, 18, and 26 of pregnancy by using in situ hybridization (ISH) and immunohistochemistry. With ISH, expression of prostasin mRNA was intensely localized in the glandular epithelium on days 12 and 18 and in the placental villi, trophoblastic column, trophoblastic shell, and fetal-maternal border on days 18 and 26. With the progress of pregnancy, expression level in the glandular epithelium was significantly decreased, and the accumulation in the placental compartments was further increased. In addition, the stroma and arterioles exhibited modest levels of prostasin signals. However, expression level of PN-1 in these compartments on adjacent sections in the three stages of early pregnancy was weak or below the level of detection. Prostasin protein expression in the endometrium was found to be consistent with the distribution patterns revealed in the ISH experiments. It may be suggested from these results that prostasin is involved in endometrial epithelial morphology establishment, tissue remodeling, and trophoblastic invasion during early pregnancy. The cognate serpin PN-1 was not coordinately expressed along with prostasin, creating a tissue environment favorable for proteolytic activities of prostasin during early pregnancy events. 相似文献
164.
Estrogen receptor- and aromatase-deficient mice provide insight into the roles of estrogen within the ovary and uterus. 总被引:1,自引:0,他引:1
Estrogen receptor (ER)- (alpha, beta, and both alpha and beta) and aromatase (Ar) knockout (KO) mice have been created to assess the biological effects of estrogens. This review article discusses the ovarian and uterine phenotypes of these mice. The data obtained have confirmed some older inferences about how the steroid acts, but have also revealed some unexpected aspects of estrogen action. Mol. Reprod. Dev. 59:336-346, 2001. 相似文献
165.
Endometrial cycle changes in adule female cynomolgus monkeys with normal ovulatory cycles were examined prospectively by real-time ultrasound. Endometrial thickness, as measured by ultrasound, was correlated with cycle day and serum estradiol (E2) and progesterone (P4) levels. We conclude that ultrasound is a reliable method of diagnosis of endometrial cycle stage. 相似文献
166.
The effect of up to 10 years of lead (Pb) exposure (1500 micrograms/kg BW/day) on endometrial cycle changes in adult cynomolgus monkeys (n = 30) were examined by real-time ultrasound. Pb exposure had no effect on peak serum estradiol (E2) or progesterone (P4) concentrations, menstrual cycle length, and endometrial response to gonadal steroids as determined by ultrasound. 相似文献
167.
Gertrude Mungyer Wim N. P. Willemsen Rune Rolland Hans M. Vemer Francs C. S. Ramaekers Paul H. K. Jap Lambert G. Poels 《In vitro cellular & developmental biology. Plant》1987,23(2):111-117
Summary Cellular elements from the mucous membrane of the uterus and oviducts and from peritoneal washings were cultured. The in vitro
behavior of these cells was compared to elucidate the histogenesis of endometriosis and the role of various diagnostic procedures.
In 65% of the cultured matrial obtained by uterine-tubal flushing, proliferating cells of the uterine-tubal mucous membrane
were present. Their morphology and behavior corresponded to those of cultured cells obtained by separate washing of the uterine
cavity and the tubes, respectively, curetted material, and biopsies of endometriosis lesions.
Epithelial and stromal cells were identified using phase contrast microscopy, electron microscopy, and immunohistochemical
methods. These cell types did not occur in peritoneal washings before the flushing of uterus and tubes. It was therefore assumed
that they were detached and transported to the pelvic cavity during the above-mentioned procedures. In view of their intensive
proliferation they may form the basis in the development of nodules of endometriosis. This would support the implantation
theory concerning the pathogenesis of endometriosis. Interactions between epithelial and mesothelial cells point to the possible
role of the latter in encapsulating the endometrial elements. 相似文献
168.
S. Tabibzadeh K. L. Kaffka P. L. Kilian P. G. Satyaswaroop 《In vitro cellular & developmental biology. Plant》1990,26(12):1173-1179
Summary Recent studies suggest that the proliferation and expression of HLA-DR molecules in endometrial epithelium may be regulated
by systemic steroids and local cytokines. To test the interacting influences of cytokines and steroids on the expression of
HLA-DR and proliferation of epithelial cells, an endometrial cell model is required that is sensitive to both signals. In
this study, we characterize cells of carcinoma cell lines of endometrial lineage for their responsiveness to cytokines and
steroids. Independently developed for its response to steroid hormones from a well-differentiated adenocarcinoma of human
endometrium, EnCa101AE cell line is further cloned for the expression of progesterone receptor. Immunohistochemical localization
using monoclonal antibodies demonstrates that both EnCa101AE cell line and cloned ECC1 cells are purely epithelial, as evidenced
by the expression of cytokeratin and epithelial membrane antigen, express estrogen receptors, and concomitantly exhibit IFN-gamma
receptor. Experiments using radioiodinated IL-1 reveal that these cell lines also possess high affinity receptors for IL-1.
As indicated by the induction of HLA-DR molecules, and alterations in morphologic characteristics, these cell lines are sensitive
to both IFN-gamma and IL-1 action. The class II molecules (HLA-DR, HLA-DP, and HLA-DQ) are differentially induced by IFN-gamma
treatment in carcinoma cell lines, with HLA-DR being the prevailing induced molecule. IFN-gamma inhibits and estradiol-17β
promotes growth of ECC1 cells in a dose-and time-dependent manner. These findings indicate that the interacting effect(s)
of the cytokines and steroid hormones on endometrial epithelium may be studied in these unique steroid-and cytokine-sensitive
epithelial cell lines. 相似文献
169.
Summary The growth of short-term primary cultures of endometrial epithelium has been studied using Feulgen microspectrophotometry.
A gradual increase in the number of polyploid nuclei up to 64C has been observed and is associated with a decline in the growth
capacity of the cultures. The specific mechanism(s) of this polyploidization is not known. 相似文献
170.
Ivone Castro Marco Antonio Cerbn Ana Maria Pasapera Ruben GutiRrez-sagal Gustavo A. Garcia Carlos Orozco Ignacio Camacho-Arroyo Rene Anzaldua Gregorio PRez-Palacios 《Molecular reproduction and development》1995,40(2):157-163
Norethisterone (NET) has been used as a contragestational postcoital agent. It is biotrans-formed to 5α dihydro-NET (5α-NET) and 3β,5α tetrahydro-NET (3β,5α-NET) in target tissues. The participation of these metabolites in NET effects is unknown. We have examined the antiimplantation and antiprogestational effects of NET and its metabolites, in adult mated female rabbits, by assessing the number of implantation sites and the expression products of the uteroglobin (UTG) gene in the uterus, and by comparing them with those of RU-486 and estradiol. Steroids were daily administered s.c. at several doses for 7 consecutive days, starting 24 hr after coitus. To assure that fertilization occurred in all animals, the presence of early pregnancy factor was determined. The results demonstrated that high doses (5 mg/kg) of NET reduced both implantation and the expression of the UTG gene. On the other hand, lower doses (1.5 mg/kg) of 5α-NET produced an antiimplantation effect and suppressed UTG synthesis and its mRNA. These effects were similar to those of RU-486. At lower doses (1 mg/kg), both estradiol and the estrogenic metabolite 3β,5α-NET were also effective in inhibiting implantation and UTG gene expression. The overall results suggest that NET metabolites exert antiimplantation and antiprogestational effects through their interaction with progesterone and estrogen receptors, and provide an explanation for the molecular mechanisms involved in the postcoital contraceptive action of NET. © 1995 Wiley-Liss, Inc. 相似文献