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11.
《Saudi Journal of Biological Sciences》2020,27(9):2420-2424
Dodonaea viscosa (Sapindaceae) is widespread in the mountainous highlands of the southwestern part of Kingdom of Saudi Arabia, where it is a medicinally important species for the people in Saudi Arabia. Seeds of this species were collected from Mount Atharb in Al-Baha region, at an altitude of 2100 m. The aims of this study were to determine if the seeds of D. viscosa have physical dormancy (i.e. a water-impermeable seed coat) and, if so, what treatments would break dormancy, and what conditions promote germination after dormancy has been broken. The dormancy-breaking treatments included: soaking of seeds in concentrated sulfuric acid (H2SO4) for 10 min, immersion in boiling water for 10 min and exposure to 50 °C for 1 min. After seeds had been pre-treated with H2SO4, to break dormancy, they were incubated at constant temperatures from 5 to 35 °C, under 12-h photoperiods or in continuous darkness, and germination recorded. Salinity tolerance was investigated by incubating acid-scarified seeds in different concentrations of mM NaCl in the light at 25 °C.Untreated seeds had low final germination 30%. Seeds that had been acid-scarified, immersed in boiling water or exposed to 50 °C all achieved 91% subsequently when incubated at 25 °C. Thus, seeds of this species in Saudi Arabia have physical dormancy, which can be broken by all three treatments designed to increase the permeability of the testa. After pre-treatment, there was a broad optimum constant temperature for germination that ranged between 5 and 25 °C but germination was inhibited by higher temperatures (30 and 35 °C). Light had little effect on this germination response. Scarified seeds were also sensitive to salinity, with the highest germination in distilled water and complete inhibition in 400 mM NaCl. Seeds that failed to germinate in saline treatments were mostly able to germinate on transfer to distilled water, suggesting osmotic inhibition. 相似文献
12.
The aquatic duckweed Spirodela polyrhiza propagates itself vegetatively by forming turions – bud‐like perennation organs – in the autumn, which spend the winter on the bottom of ponds and then germinate in the following spring and proliferate on the water surface. Newly formed turions usually require a period of cold after‐ripening and light to germinate effectively, but an ample supply of exogenous sugar can lead to germination even in the dark and independent of after‐ripening. The results of the present study indicate that the availability of readily metabolised carbohydrates is a determining factor for turion germination. Freshly harvested turions do not contain soluble, low‐molecular weight carbohydrates at a level sufficient to allow germination to take place, but after‐ripened turions do. Augmentation of the soluble carbohydrate content during after‐ripening derives from gradual breakdown of reserve starch of the turions. The long time required for any germination to be observed in turions incubated in darkness and the limited frequency of germination in the dark (about 50% of turion population), even with an ample external sugar, supply emphasise that both after‐ripening and light are essential for ensuring rapid germination and subsequent frond proliferation at an ecologically appropriate time. The carbohydrate supply required for rapid proliferation of the fronds produced at germination is provided by the rapid light‐induced breakdown of turion reserve starch. 相似文献
13.
Dormancy of<Emphasis Type="Italic"> Arabidopsis</Emphasis> seeds and barley grains can be broken by nitric oxide 总被引:13,自引:0,他引:13
Seeds of Arabidopsis thaliana (L.) Heynh. and grains of barley (Hordeum vulgare L.) were used to characterize the affects of nitric oxide (NO) on seed dormancy. Seeds of the C24 and Col-1 ecotypes of Arabidopsis are almost completely dormant when freshly harvested, but dormancy was broken by stratification for 3 days at 4°C or by imbibition of seeds with the NO donor sodium nitroprusside (SNP). This effect of SNP on dormancy of Arabidopsis seeds was concentration dependent. SNP concentrations as low as 25 M reduced dormancy and stimulated germination, but SNP at 250 M or more impaired seedling development, including root growth, and inhibited germination. Dormancy was also reduced when Arabidopsis seeds were exposed to gasses that are generated by solutions of SNP. Nitrate and nitrite, two other oxides of nitrogen, reduced the dormancy of Arabidopsis seeds, but much higher concentrations of these were required compared to SNP. Furthermore, the kinetics of germination were slower for seeds imbibed with either nitrate or nitrite than for seeds imbibed with SNP. Although seeds imbibed with SNP had reduced dormancy, seeds imbibed with SNP and abscisic acid (ABA) remained strongly dormant. This may indicate that the effects of ABA action on germination are downstream of NO action. The NO scavenger 2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3 oxide (cPTIO) strengthened dormancy of unstratified and briefly stratified Arabidopsis seeds. Dormancy of three cultivars of barley was also reduced by SNP. Furthermore, dormancy in barley grain was strengthened by imbibition of grain with cPTIO. The data presented here support the conclusion that NO is a potent dormancy breaking agent for seeds and grains. Experiments with the NO scavenger suggest that NO is an endogenous regulator of seed dormancy.Abbreviations ABA Abscisic acid - cPTIO 2-(4-Carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3 oxide - GA Gibberellin - SNP Sodium nitroprusside - NOx Gaseous oxides of nitrogen 相似文献
14.
Vorwerk S Biernacki S Hillebrand H Janzik I Müller A Weiler EW Piotrowski M 《Planta》2001,212(4):508-516
Three of the nitrilase isoenzymes of Arabidopsis thaliana (L.) Heynh. are located on chromosome III in tandem and these genes (NIT2/NIT1/NIT3 in the 5′→3′ direction) encode highly similar polypeptides. Copy DNAs encompassing the entire coding sequences for all three
nitrilases were expressed in Escherichia coli as fusion proteins containing a C-terminal hexahistidine extension. All three nitrilases were obtained as enzymatically active
proteins, and their characteristics were determined, including a detailed comparative analysis of their substrate preferences.
All three nitrilases converted indole-3-acetonitrile (IAN) to indole-3-acetic acid (IAA), albeit, compared to the most effective
substrates found, phenylpropionitrile (PPN), allylcyanide, (phenylthio)acetonitrile and (methylthio)acetonitrile, with low
affinity and velocity. The preferred substrates are either naturally occurring substrates, which may originate from glucosinolate
breakdown, or they are close relatives of these. Thus, a major function of NIT1, NIT2 and NIT3 is assigned to be the conversion
to carboxylic acids of nitriles from glucosinolate turnover or degradation. While all nitrilases exhibit a similar pH optimum
around neutral, and NIT1 and NIT3 exhibit a similar temperature optimum around 30 °C independent of the substrate analyzed
(IAN, PPN), NIT2 showed a remarkably different temperature optimum for IAN (15 °C) and PPN (35–40 °C). A potential role for
NIT2 in breaking seed dormancy in A. thaliana by low temperatures (stratification), however, was ruled out, although NIT2 was the predominantly expressed nitrilase isoform
in developing embryos and in germinating seeds, as judged from an analysis of β-glucuronidase reporter gene expression under
the control of the promoters of the four isogenes. It is possible that NIT2 is involved in supplying IAA during seed development
rather than during stratification.
Received: 13 May 2000 / Accepted: 14 August 2000 相似文献
15.
Genes associated with the end of dormancy in grapes 总被引:11,自引:0,他引:11
Pacey-Miller T Scott K Ablett E Tingey S Ching A Henry R 《Functional & integrative genomics》2003,3(4):144-152
A grape bud EST library was constructed and 4,270 ESTs sequenced. The library clones were arrayed for the purpose of investigating the level of gene expression over time, particularly leading up to the buds release from dormancy. The arrays were hybridized with P33-labeled probes produced from samples of buds collected at weekly intervals. These probes covered the time from 9 weeks prior to bud burst until just after the emergence of the shoots. Expression patterns from these genes have been examined. It was found that 74% of the genes in the data set were homologous to known proteins. Genes were then assigned to functional categories according to their primary BLAST match. Of these 13% were involved with photosynthesis, 13% with disease resistance and defense, 5% energy, 12% metabolism, 20% protein production and processing, 25% cell structure and plant growth and the remaining 12% were unclassified The expression pattern of a selection of candidate genes retrieved from literature previously reporting an association with dormancy changes was assessed. On closer examination most of these genes relate to the oxidative processes and stress responses within the cell. The results of this study show that even in the dormant state, gene expression in the buds is high. 相似文献
16.
The control of field tuber dormancy in the yam (Dioscorea cayenensis–D. rotundata complex) is poorly understood. Although studies have examined single environmental factors and chemical treatments that might prolong tuber dormancy and storage, only a few were focused on further tuber sprouting. The present study concerns microtubers obtained by in vitro culture. When microtubers were harvested (after 9 months of culture) and directly transferred on a new medium without hormones, the tubers rapidly sprouted in in vitro conditions. No dormancy was observed in this case. Harvested microtubers were also stored dry in jars in sterile conditions during 2 to 18 weeks before in vitro sprouting. In this case, microtubers stored during 18 weeks sprouted more rapidly than those stored 8 weeks. A constant “dormancy-like period” (storage duration + sprouting delay) was observed, between 20 and 28 weeks respectively for the more rapid and the slower microtubers. The size of the tubers used for the storage had great influence on further sprouting. The larger they were, the better they sprouted. Light during storage had no effect on the sprouting delay while a temperature of 25 °C permit a quicker sprouting than 18 °C. The medium used to obtain microtubers could also have an effect on sprouting rate. 相似文献
17.
18.
Zaffryar S Zimerman B Abu-Abied M Belausov E Lurya G Vainstein A Kamenetsky R Sadot E 《Protoplasma》2007,230(3-4):153-163
Summary.
Narcissus tazetta is one of the major geophyte crops worldwide, but little is known about its cell biology. The narcissus storage organ was
studied by monitoring scale cell biology during the growth stage and dormancy, and it was found that amyloplasts gradually
increased in size and reached a maximum at dormancy. In parallel, microtubules changed their organisation: during the growth
phase (February to March) they were oblique; during April and May, microtubules formed a network with round “holes”; by late
June and the beginning of July, when dormancy started, they were organised in parallel arrays. The holes formed in the microtubule
array corresponded to amyloplasts. A closer look showed that during a short time window, while the plants were preparing for
dormancy, the microtubules surrounded the amyloplasts. In vitro reconfirmation of this phenomenon was obtained when fluorescent
bovine brain microtubules enwrapped isolated amyloplasts that had been purified between April and July but not those purified
between January and March. Interestingly, protease treatment of amyloplasts did not completely prevent binding of microtubules,
which suggests the existence of a protease-resistant factor that docks microtubules to the outer membrane of amyloplasts.
Correspondence and reprints: Department of Ornamental Horticulture, Volcani Center, Bet Dagan 50250, Israel. 相似文献
19.
Photoperiodic control of diapause termination, colour change and postdiapause reproduction in the southern green stink bug, Nezara viridula 总被引:1,自引:0,他引:1
Abstract. The effects of day length on adult diapause development, associated with diapause body colour change as well as postdiapause reproduction are studied in Nezara viridula from Japan. Facultative diapause spontaneously terminates under three constant short-day and near-critical photoperiods at 25 °C without low temperature treatment. The period required for body colour change from russet to green and the precopulation and preoviposition periods differ significantly between the photoperiodic treatments, being shortest under LD 13 : 11 h, intermediate under LD 12 : 12 h and longest under LD 10 : 14 h. Photoperiodic conditions do not affect postdiapause reproductive performance: the total egg production, duration of the period of oviposition and other reproductive indices do not differ significantly between the photoperiodic conditions. The total egg production depends on the duration of the period of oviposition but not on how long females remained russet during diapause. It is concluded that diapause in N. viridula does not require low temperature for its successful completion and diapause duration affects winter survival but not postdiapause reproductive performance or longevity. Such independence of the postdiapause reproductive performance from the duration of diapause may have contributed to the continuous worldwide range expansion of this species into temperate zone. 相似文献
20.
弥勒苣苔为中国特有的濒危植物,一度被认为灭绝后又被重新发现,引起了学界及公众的广泛关注.种子库是保护珍稀濒危物种的一种有效手段,对物种开展种子保存需要较为深入的了解其种子休眠及萌发特性;但对于弥勒苣苔,现有资料十分有限,本研究系统地探讨了弥勒苣苔种子的休眠状态及萌发习性.在对其种子进行X光透视后,发现其种子的胚发育完全,且基本充满整个种子,因此认为该种子不具有形态休眠.在多个变温及恒温条件下对新鲜种子进行萌发测试,发现在较高温度(高于20℃)下萌发率很高且萌发迅速,赤霉素及低温层积处理能够显著提高种子在较低温度下(低于20℃)的萌发率,因此可以判定其种子存在浅的生理休眠.种子在恒温下的萌发率比相应的变温下萌发率要高,可能是其对所处荫蔽环境的一种适应.硝酸钾对种子萌发有显著的促进作用,在野外环境下,硝酸钾有对生境空窗及较少竞争的指示作用. 相似文献