全文获取类型
收费全文 | 6910篇 |
免费 | 564篇 |
国内免费 | 1030篇 |
出版年
2024年 | 15篇 |
2023年 | 112篇 |
2022年 | 132篇 |
2021年 | 185篇 |
2020年 | 247篇 |
2019年 | 234篇 |
2018年 | 258篇 |
2017年 | 237篇 |
2016年 | 259篇 |
2015年 | 278篇 |
2014年 | 378篇 |
2013年 | 619篇 |
2012年 | 258篇 |
2011年 | 330篇 |
2010年 | 270篇 |
2009年 | 373篇 |
2008年 | 366篇 |
2007年 | 363篇 |
2006年 | 369篇 |
2005年 | 290篇 |
2004年 | 269篇 |
2003年 | 270篇 |
2002年 | 228篇 |
2001年 | 206篇 |
2000年 | 143篇 |
1999年 | 153篇 |
1998年 | 128篇 |
1997年 | 105篇 |
1996年 | 119篇 |
1995年 | 113篇 |
1994年 | 109篇 |
1993年 | 119篇 |
1992年 | 78篇 |
1991年 | 119篇 |
1990年 | 66篇 |
1989年 | 63篇 |
1988年 | 56篇 |
1987年 | 61篇 |
1986年 | 57篇 |
1985年 | 68篇 |
1984年 | 67篇 |
1983年 | 53篇 |
1982年 | 60篇 |
1981年 | 51篇 |
1980年 | 37篇 |
1979年 | 27篇 |
1978年 | 29篇 |
1977年 | 18篇 |
1976年 | 16篇 |
1975年 | 11篇 |
排序方式: 共有8504条查询结果,搜索用时 136 毫秒
51.
W. L. C. Vaz D. Hallmann R. M. Clegg A. Gambacorta M. De Rosa 《European biophysics journal : EBJ》1985,12(1):19-24
We have used the fluorescence recovery after photobleaching technique to study the translational diffusion, in L phase multibilayers of 1-palmitoyl-2-oleoylphosphatidylcholine (POPC), of fluorescent derivatives of 1-palmitoyl-2-oleoylphosphatidylethanolamine (NBD-POPE) and a membrane-spanning phosphatidylethanolamine (NBD-MSPE). The latter derivative was prepared from a membrane-spanning glycerol-dialkyl-glycerol tetraether lipid isolated from the thermophilic and acidophilic archaebacterium Sulfolobus solfataricus. The translational diffusion was examined between about 15° and 45°C. It is shown that over this temperature range the translational diffusion coefficient for NBD-MSPE is 2/3 that for NBD-POPE which spans only one monolayer of the bilayer. The result is interpreted in terms of existing models for translational diffusion in lipid membranes.Abbreviations D
t
translational diffusion coefficient
- FRAP
fluorescence recovery after photobleaching
- MSPE
a membrane-spanning phosphatidylethanolamine derived from a glycerol-dialkyl-glycerol tetraether lipid isolated from Sulfolobus solfataricus
- NBD
4-nitrobenz-2-oxa-1,3-diazolyl
- PE
phosphatidylethanolamine
- POPC
1-palmitoyl-2-oleoylphosphatidylcholine
- POPE
1-palmitoyl-2-oleoylphosphatidylethanolamine 相似文献
52.
Using isolated pea thylakoids, the relative rate of QA
- oxidation has been estimated under various conditions, from the restoration of the induction curves following a dark period and from light 1-induced changes in modulated chlorophyll fluorescence excited by light 2.Alterations of
QinfA
sup–
oxidation rates were observed under conditions which affected the degree of thylakoid stacking, the lipid fluidity and the integrity of the membranes. The results are discussed in terms of the interactions between QA
- and the plastoquinone pool with particular emphasis on lateral diffusion.Abbreviations DCMU
3-(3,4-dichlorophenyl)-1,1-dimethylurea
- EDTA
Ethylenediaminetetracetate
- Hepes
N-2-hydroxyethyl piperazine-N-2-ethanesulphonic acid
- NADP
nicotinamide adenine dinucleotide phosphate 相似文献
53.
R. J. HELDER 《Plant, cell & environment》1985,8(6):399-408
Abstract The quantitative approach used here is based on a model comprising a well-stirred medium, an unstirred layer, and a CO2 absorbing leaf. The unstirred layer is divided up by planes into a number of sub-layers. Within each plane the concentration of each solute is everywhere the same as is the electric potential. These variables constitute the basic data. Thus the planes were characterized by their pH value. An equation is derived which enables the calculation of the basic data of a plane from the known data of another plane. In this way it is possible to calculate the basic data for all planes. From these data the rate of assimilation, the thickness of the unstirred layer and its sub-layers, the fluxes across the sub-layers and the conversions among the carbon components can be estimated. The CO2 flux decreases, and the HCO?3 flux increases towards the leaf. There are negative fluxes of OH& and CO2–3. H+ fluxes are of minor importance and can be ignored if the pH of the medium is higher than 8.0, provided no non-inorganic C buffers with appropriate pKa are present. The significance of the carbon diffusion facilitating effect of an inorganic carbon system is expressed in various ways. The values obtained represent maxima, as the assumption is made that the equilibrium reactions are very fast. It is argued that even better effects are possible if the back-diffusion of CO2–3 could be prevented by lowering the pH of the unstirred layer. 相似文献
54.
CO2 uptake and transport in leaf mesophyll cells 总被引:4,自引:3,他引:1
Abstract The acquisition of inorganic carbon for photosynthetic assimilation by leaf mesophyll cells and chloroplasts is discussed with particular reference to membrane permeation of CO2 and HCO?3. Experimental evidence indicates that at the apoplast pH normally experienced by leaf mesophyll cells (pH 6–7) CO2 is the principal species of inorganic carbon taken up. Uptake of HCO?3 may also occur under certain circumstances (i.e. pH 8.5), but its contribution to the net flux of inorganic carbon is small and HCO?3 uptake does not function as a CO2-concentrating mechanism. Similarly, CO2 rather than HCO?3 appears to be the species of inorganic carbon which permeates the chloroplast envelope. In contrast to many C3 aquatic plants and C4 plants, C3 terrestrial plants lack specialized mechanisms for the acquisition and transport of inorganic carbon from the intercellular environment to the site of photosynthetic carboxylation, but rely upon the diffusive uptake of CO2. 相似文献
55.
Abstract A most-probable-number technique was used to quantify the abundance of myxomycetes (myxogastrids) in soil samples taken from 23 'Sites of Special Scientific Interest' and one other site in the West of England. Associated organisms and soil conditions were also recorded. Sixteen of the 24 sites yielded myxomycete plasmodium-forming units (PFUs) in numbers averaging 230 cm−3 fresh soil. Where detectable in samples of grassland soil, the numbers ranged from 20–2750 cm−3 , in woodland soil from 20–800 cm−3 , and in sand dunes from 80–400 cm−3 . Repeated sampling revealed changing numbers at single sites. The abundance of PFUs was correlated positively with numbers of soil amoebae, ciliates and nematodes, and with levels of magnesium and soil density, and negatively with organic matter content and levels of NH4 -nitrogen and phosphate. Each PFU probably corresponded to one or several uninucleate cells in the soil rather than to a plasmodium. 相似文献
56.
Estimation in change-point hazard rate models 总被引:1,自引:0,他引:1
57.
Peter H. Barry 《The Journal of membrane biology》1984,82(3):221-239
Summary Many neurones are extremely invaginated and possess branching processes, axons and dendrites. In general, they are surrounded by a restricted diffusion space. Many of these cells exhibit large, slow potential changes during the passage of current across their membranes. Whenever currents cross membranes separating aqueous solutions, differences in transport numbers of the major permeant ions give rise to local concentration changes of these ions adjacent to the membranes, which will result in various electrical and osmotic effects. These transport number effects are expected to be enhanced by the presence of membrane invaginations. Dendrites are equivalent to reversed invaginations and there should be significant changes in concentrations of permeant ions within them. In general, the effects of such changes on the electrical response of a cell will be greater when the concentration of a major permeant ion is low. The effects have been modelled in terms of two nondimensional parameters: the invagination transport number parameter and the relative area occupied by the invaginations A. If these two parameters are known, the magnitudes and time course of the slow potential changes can immediately be estimated and the time course converted to real time, if the length of the invaginations (l) and ionic diffusion coefficient (D) within them are also known. Both analytical and numerical solutions have been given and predictions compared. It is shown that in the case of large currents and potentials the analytical solution predictions will underestimate the magnitudes and rates of onset of the voltage responses. The relative magnitude of the transport number effect within the invaginations (or dendrites) and other transport number contributions to slow potential changes have also been assessed and order-of-magnitude values of these are estimated for some biological data. 相似文献
58.
A. Shteyer D. Gazit I. Binderman I. A. Bab 《In vitro cellular & developmental biology. Plant》1987,23(1):15-20
Summary Cells of the dental papilla are capable of odontoblastic, fibroblastic, and endothelial differentiation and formation of dentin
and the dental pulp. In the present study dental papilla cells, obtained from human tooth buds (HDP cells), were cultured
in vitro through 3 to 7 passages. After exposure to prostaglandin E2 there was a marked decrease in intracellular cyclic AMP (cAMP) levels as compared to hormone-free controls. Parathyroid hormone
and calcitonin had stimulatory effects with 1 and 2 log increases in cAMP, respectively. The HDP cells showed moderate activity
of alkaline phosphatase, 1 log higher than that of hamster kidney fibroblasts (BHK 13) and 1 log lower than that of osteoblastic
osteosarcoma cells (ROS 17/2). When cultured for 4 or 8 wk in diffusion chambers (DC) implanted in athymic mice, many of the
HDP cells underwent odontoblastic morphodifferentiation with very long, single processes extending into the matrix. This matrix
contained banded and unbanded collagen fibers. Neither light nor electron microscopy of the DC content revealed mineral deposits.
These results suggest that HDP cells have an intrinsic potential for partial odontoblastic differentiation; inductive signals
like those originating from odontogenic epithelium are probably essential for the completion of hard tissue formation. 相似文献
59.
A sperm penetrates an egg by extending a long, actin-filled tube known as the acrosomal process. This simple example of biomotility is one of the most dramatic. In Thyone, a 90 m process can extend in less than 10 s. Experiments have shown that actin monomers stored in the base of the sperm are transported to the growing tip of the acrosomal process where they add to the ends of the existing filaments.The force that drives the elongation of the acrosomal process has not yet been identified although the most frequently discussed candidate is the actin polymerization reaction. Developing what we believe are realistic moving boundary models of diffusion limited actin fiber polymerization, we show that actin filament growth occurs too slowly to drive acrosomal elongation. We thus believe that other forces, such as osmotically driven water flow, must play an important role in causing the elongation. We conjecture that actin polymerization merely follows to give the appropriate shape to the growing structure and to stabilize the structure once water flow ceases.Work partially supported by the United States Department of Energy 相似文献
60.
A functional differential equation which is nonlinear and involves forward and backward deviating arguments is solved numerically. The equation models conduction in a myelinated nerve axon in which the myelin completely insulates the membrane, so that the potential change jumps from node to node. The equation is of first order with boundary values given at t=±. The problem is approximated via a difference scheme which solves the problem on a finite interval by utilizing an asymptotic representation at the endpoints, cubic interpolation and iterative techniques to approximate the delays, and a continuation method to start the procedure. The procedure is tested on a class of problems which are solvable analytically to access the scheme's accuracy and stability, then applied to the problem that models propagation in a myelinated axon. The solution's dependence on various model parameters of physical interest is studied. This is the first numerical study of myelinated nerve conduction in which the advance and delay terms are treated explicitly.Supported in part by NSF Grant MCS8301724 and by a Biomedical Research Support Grant 2SO7RR0706618 from NIH 相似文献