全文获取类型
收费全文 | 4632篇 |
免费 | 201篇 |
国内免费 | 276篇 |
出版年
2024年 | 6篇 |
2023年 | 31篇 |
2022年 | 98篇 |
2021年 | 139篇 |
2020年 | 117篇 |
2019年 | 131篇 |
2018年 | 85篇 |
2017年 | 71篇 |
2016年 | 74篇 |
2015年 | 206篇 |
2014年 | 352篇 |
2013年 | 332篇 |
2012年 | 487篇 |
2011年 | 535篇 |
2010年 | 408篇 |
2009年 | 170篇 |
2008年 | 231篇 |
2007年 | 170篇 |
2006年 | 154篇 |
2005年 | 137篇 |
2004年 | 109篇 |
2003年 | 99篇 |
2002年 | 98篇 |
2001年 | 79篇 |
2000年 | 72篇 |
1999年 | 64篇 |
1998年 | 46篇 |
1997年 | 44篇 |
1996年 | 70篇 |
1995年 | 50篇 |
1994年 | 57篇 |
1993年 | 46篇 |
1992年 | 39篇 |
1991年 | 40篇 |
1990年 | 38篇 |
1989年 | 38篇 |
1988年 | 20篇 |
1987年 | 20篇 |
1986年 | 18篇 |
1985年 | 18篇 |
1984年 | 17篇 |
1983年 | 6篇 |
1982年 | 14篇 |
1981年 | 14篇 |
1980年 | 8篇 |
1979年 | 11篇 |
1978年 | 5篇 |
1977年 | 11篇 |
1976年 | 6篇 |
1973年 | 6篇 |
排序方式: 共有5109条查询结果,搜索用时 15 毫秒
81.
82.
The distribution of virus-infected cells was examined, by fluorescence microscopy, within plants of a range of potato clones infected with potato leafroll luteovirus (PLRV). This range included nine PLRV-resistant clones, of which four were transgenic lines carrying the PLRV coat protein gene and five were conventionally bred. Plants of these clones were resistant to PLRV multiplication and accumulated less virus antigen in leaf tissue than did susceptible clones. Indirect fluorescent antibody staining of thin sections from carbodiimide-fixed petiole tissue revealed that in plants of PLRV-susceptible clones, virus-infected cells were abundant within both external (abaxial) and internal (adaxial) phloem bundles. In plants of the PLRV-resistant conventionally bred clones and in resistant transgenic lines of cv. Pentland Squire, virus-infected cells were much fewer in number and largely restricted to internal phloem bundles. In resistant transgenic lines of cv. Désirée, this restricted distribution of PLRV antigen was only detected in petioles of young leaves. The results suggest that the transgenic and a host-mediated type of resistance that restricts virtis multiplication have underlying similarities. 相似文献
83.
84.
85.
马铃薯卷叶病毒基因间隔区的克隆及序列分析 总被引:6,自引:0,他引:6
根据已报道的马铃薯卷叶病毒基因组序列.设计合成一对特异性引物,以马铃薯卷叶病毒中国分离株(PLRV-Ch)的RNA为模板,反转录合成cDNA第一条链,经PCR扩增后克隆于pUC19质粒中,进一步用PCR鉴定、限制酶切分析和序列分析,结果表明:PLRV-Ch基因间隔区由197个核苷酸组成,与国外报道的荷兰PLRV-N加拿大PLRV-C,澳大利亚PLRV-A,苏格兰PLRV-S各株系核苷酸序列具有很高的同源性,同源率依次为99%、98%、93%、98%。 相似文献
86.
韭菜病毒分离物初步鉴定房德纯,王振东,佟成富,陆庆轩(沈阳农业大学植保系,沈阳110161)(辽宁省风沙地改良利用研究所,阜新123000)(沈阳市园林科学研究院,沈阳110015)关键词韭菜病毒病,毒原鉴定,韭菜萎缩病毒1993年在辽宁省阜新市韭菜... 相似文献
87.
用生物活性法和双抗体夹心桥联酶免疫吸附(ELISA)法检测了人类疱疹病素6型(HHV-6)GS株和南京地方株CN5,8,10感染的淋巴细胞培养上清中的肿瘤坏死因子(TNF)的水平,发现培养24h即可检出高水平的TNF,48~72h述到峰值,此后逐渐下降,与未感染耐照组比较有及其显著的差异(P<0.001)。GS株与地方株同诱生TNF水平无儿著性差异(P>0.1),三株地方株诱生TNF也无显著性差异(P>0.05)。TNF-α单抗可以完全中和培养上清中TNF的活性,证实上清中有TNF-α。与LPS比较,HHV-6诱生TNF-α的能力要强得多。 相似文献
88.
Characterization of quantitative trait loci (QTLs) in cultivated rice contributing to field resistance to sheath blight (Rhizoctonia solani) 总被引:1,自引:0,他引:1
Zhikang Li S. R. M. Pinson M. A. Marchetti J. W. Stansel W. D. Park 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1995,91(2):382-388
Sheath blight, caused by Rhizoctonia solani, is one of the most important diseases of rice. Despite extensive searches of the rice germ plasm, the major gene(s) which give complete resistance to the fungus have not been identified. However, there is much variation in quantitatively inherited resistance to R. solani, and this type of resistance can offer adequate protection against the pathogen under field conditions. Using 255 F4 bulked populations from a cross between the susceptible variety Lemont and the resistant variety Teqing, 2 years of field disease evaluation and 113 well-distributed RFLP markers, we identified six quantitative trait loci (QTLs) contributing to resistance to R. solani. These QTLs are located on 6 of the 12 rice chromosomes and collectively explain approximately 60% of the genotypic variation or 47% of the phenotypic variation in the LemontxTeqing cross. One of these resistance QTLs (QSbr4a), which accounted for 6% of the genotypic variation in resistance to R. solani, appeared to be independent of associated morphological traits. The remaining five putative resistance loci (QSbr2a, QSbr3a, QSbr8a, QSbr9a and QSbr12a) all mapped to chromosomal regions also associated with increased plant height, three of which were also associated with QTLs causing later heading. This was consistent with the observation that heading date and plant height accounted for 47% of the genotypic variation in resistance to R. solani in this population. There were also weak associations between resistance to R. solani and leaf width, which were likely due to linkage with a QTL for this trait rather than to a physiological relationship. 相似文献
89.
Ana María Bravo-Angel Heinz-Albert Becker Reinhard Kunze Barbara Hohn Wen-Hui Shen 《Molecular & general genetics : MGG》1995,248(5):527-534
A reverse genetic system for studying excision of the transposable elementDs1 in maize plants has been established previously. In this system, theDs1 element, as part of the genome of maize streak virus (MSV), is introduced into maize plants via agroinfection. In the presence
of theAc element, excision ofDs1 from the MSV genome results in the appearance of viral symptoms on the maize plants. Here, we used this system to study DNA
sequences requiredin cis for excision ofDs1. TheDs1 element contains theAc transposase binding motif AAACGG in only one of its subterminal regions (defined here as the 5′ subterminal region). We showed
that mutation of these motifs abolished completely the excision capacity ofDs1. This is the first direct demonstration that the transposase binding motifs are essential for excision. Mutagenesis with
oligonucleotide insertions in the other (3′) subterminal region resulted in elements with either a reduced or an increased
excision efficiency, indicating that this subterminal region also has an important function. 相似文献
90.
Impact of dengue virus infection and its control 总被引:3,自引:0,他引:3
Akira Igarashia 《FEMS immunology and medical microbiology》1997,18(4):291-300