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61.
Damian Labuda Daniel Sinnett Chantal Richer Jean-Marc Deragon George Striker 《Journal of molecular evolution》1991,32(5):405-414
Summary In a recent report mouse B1 genomic repeats were divided into six families representing different waves of fixation of B1 variants, consistent with the retroposition model of human Alu elements. These data are used to examine the distribution of nucleotide substitutions in individual genomic repeats with respect to family consensus sequences and to compare the minimal energy structures of the corresponding B1 RNAs. By an enzymatic approach the predicted structure of B1 RNAs is experimentally confirmed using as a model sequence an RNA of a young B1 family member transcribed in vitro by T7 RNA polymerase. B1 RNA preserves folding domains of the Alu fragment of 7SL RNA, its progenitor molecule. Our results reveal similarities among 7SL-like retroposons, human Alu, and rodent B1 repeats, and relate the evolutionary conservation of B1 family consensus sequences to selection at the RNA level. 相似文献
62.
Structural and genetical studies on the high-molecular-weight subunits of wheat glutenin 总被引:14,自引:2,他引:12
P. I. Payne L. M. Holt C. N. Law 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1981,60(4):229-236
Summary The high-molecular-weight (HMW) subunits of glutenin from about 185 varieties were fractionated by sodium dodecyl sulphate
polyacrylamide gel electrophoresis (SDS-PAGE). About 20 different, major subunits were distinguished by this technique although
each variety contained, with only a few exceptions, between 3 and 5 subunits. Further inter-varietal substitution lines to
those already described (Payne et al. 1980) were analysed and the results indicate that all the HMW subunits are controlled
by the homoeologous group 1 chromosomes. All hexaploid varieties studied except ‘NapHal’ contained two major subunits controlled
by chromosome 1D. Their genes were shown to be tightly linked genetically for only four different types of banding patterns
were observed. The nominal molecular weights determined after fractionation in 10% polyacrylamide gels were between 110,000
and 115,000 for the larger of the two subunits and between 82,000 and 84,000 for the smaller. One quarter of the varieties
contained only one major HMW subunit controlled by chromosome 1B whereas the rest had two. The chromosome 1B subunits were
the most varied and nine different banding patterns were detected. All the subunits had mobilities which were intermediate
between those of the two chromosome 1D-controlled subunits. Only two types of HMW subunit controlled by chromosome 1A were
detected in all the varieties examined; a single variety never contained both of these subunits and 40% of varieties contained
neither. The chromosome 1A-controlled subunits had slightly slower mobilities in 10% gels than the largest HMW subunit controlled
by chromosome 1D. About 100 single grains were analysed from each of five different crosses of the type (F1 of variety A × variety B) × variety C. The results indicate that the genes on chromosome 1B which control the synthesis of
subunits 6, 7, 13, 14 and 17 are allelic, as are the genes of the chromosome 1A-controlled subunits, 1 and 2. 相似文献
63.
A comparison was made between the induction of intragenic and intergenic recombinations during meiosis in a wild-type diploid of Saccharomyces cerevisiae. Under non-irradiated normal conditions, production of both intragenic and intergenic recombinants greatly increased in the cells with commitment to meiosis. The susceptibility of cells to the induction of both the spontaneous intra- and intergenic recombinations in meiotic cells was similar. However, under condition of UV irradiation, there were striking differences between intra- and intergenic recombinations. Susceptibility to induction of intragenic recombination by UV irradiation was not enhanced at meiosis compared with mitosis, and was not altered through commitment to meiotic processes. In contrast, however, susceptibility to the induction of intergenic recombination by UV irradiation was enhanced markedly during commitment to meiosis compared with mitosis. Genetic analysis suggested that the enhanced susceptibility to recombination during meiosis is specifically concerned with reciprocal-type recombination (crossing-over) but not non-reciprocal-type recombination (gene conversion). Hence it is concluded that the meiotic process appears to be intimately concerned with the mechanism(s) of induction of recombination, especially reciprocal-type recombination. 相似文献
64.
Madeleine Fletcher Philip T. LoVerde Charles S. Richards 《Experimental parasitology》1981,52(3):362-370
Individual adult Schistosoma mansoni from strains selected for high or low infectivity to specific strains of the snail intermediate host, Biomphalaria glabrata, were subjected to enzyme electrophoresis on starch gels. Fourteen enzyme systems were analyzed in an attempt to find electrophoretic markers associated with genes for infectivity to snails. The S. mansoni strains were selected from different isolates from Puerto Rico in several strains of B. glabrata. Of an estimated 18 loci, 3 were polymorphic and the remainder monomorphic. For 1 of the 3 polymorphic enzyme loci, lactate dehydrogenase (Ldh, EC 1.1.1.27), phenotype frequencies were correlated with infectivity to snails. In schistosome strains of low infectivity, frequencies of the Ldh-N phenotype ranged between 0.56 and 0.69, while in strains of high infectivity, Ldh-N frequencies were typically 0.91 to 1.00. Whether the correlation is accidental or due to some form of association, such as chromosomal linkage, between the locus responsible for variation in lactate dehydrogenase and a gene for infectivity to snails remains to be determined. 相似文献
65.
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67.
The aim of this investigation was to quantify the relative contributions of genetic and environmental influences to the observed variability of permanent tooth size in a group of Australian Aboriginals. Tooth size data were obtained from dental casts of Aboriginals living at Yuendumu in the Northern Territory of Australia. The custom of polygyny practised by these people enabled the analysis of associations between full-siblings and half-siblings. Phenotypic variability of tooth size was partitioned into four variance components; between sides, between fathers, between mothers and between offspring. From these components, the relative genetic and environmental contributions were quantified and heritability estimates for tooth size derived. Additional estimates of heritability were obtained by regression analysis from a small sample of parent-offspring data. Results of the analyses suggested that about 64% of the total variability of permanent tooth size could be attributed to genetic factors, while a further 6% was due to common environment. Although the findings confirm a relatively strong genetic component, they emphasise the importance of non-genetic influences in the determination of tooth size variability. 相似文献
68.
Yong-Shik Kim 《Landscape and Ecological Engineering》2006,2(2):163-170
Korea is a land of contrasts. The mountainous Korean peninsula arches north and northwest towards the Russian and Chinese borders, where the last areas of old growth coniferous forest and the land formed five forest types. Plant and habitat diversity in Korea has been largely damaged by various kinds of human activities. The plant diversity of Korea is severely threatened due to the high population density and rapid industrialization since the 1960s, as well as the illegal collection of wild plants for ornamental, medicinal, and food purposes. Natural areas have been greatly fragmented and survive only as ecological islands surrounded by modified cultivated and industrial lands. For instance, more than 60 industrial parks are located along the coast, and these have contributed to the rapid destruction of plant diversity in the coastal areas of the peninsula. The modern concept of plant conservation in Korea is still in an infant stage. The cooperation of plant conservation bodies with disciplinary approaches based on conservation biology are essential strategies to pursue plant conservation. The capacity building for plant conservation in Korea has different management regimes by the relevant ministries. Since the late 1990s, the plant conservation body Korean Plant Specialist Group of the International Union for the Conservation of Nature and Natural Resources Species Survival Commission (IUCN SSC) has been closely linked with international conservation bodies for in situ and ex situ conservation management. Under the umbrella of the national plant conservation strategies, the different strategies and working agencies can be fully integrated. Furthermore, the systematic approaches for the recovery of threatened species, as well as habitat monitoring over the long-term basis, will need to be supported by a stable budget policy. Also, protocol for the conservation of rare and endangered plant species, including recovery works in Korea, is strongly needed. 相似文献
69.
Hybridization, natural or artificial, is considered disadvantageous for species biodiversity when it threatens the population integrity of endangered species. Frequently, studies investigating whether hybridization poses a legitimate risk to rare species are based on genetic data obtained in molecular biology laboratories. In this study, we used field research to approach the problem that hybridization could cause for the viability of a population of a rare species and to be able to propose the most appropriate initial conservation strategy. Specifically, using the model genus Antirrhinum, the reproductive barriers between the rare A. pulverulentum and its common congener A. litigiosum have been analysed under the reproductive isolation index (RI). A. pulverulentum had a high value of total RI, indicating that there are barriers to gene flow from A. litigiosum towards this species, and also had a high value for the intrinsic RI, reflecting a low inherent capacity for production of hybrid plants; in addition, the possibility of successful backcrosses between this species with hybrids produced from A. litigiosum ovules were low, given the high intrinsic RI of A. litigiosum. These data indicate the current existence of strong and permanent barriers to hybridization between the two species, suggesting that hybridization does not seem to be a serious problem for the conservation of A. pulverulentum in the studied population, nor for the near future. This study shows how the RI index can provide useful information for conservation purposes and proposes different management recommendations. 相似文献
70.
A report on the 46th annual PopGroup conference, Glasgow, UK, December 18-21,
2012. 相似文献