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11.
Ophélie Vacca Brahim El Mathari Marie Darche José-Alain Sahel Alvaro Rendon Deniz Dalkara 《Journal of visualized experiments : JoVE》2015,(98)
Müller cells are the principal glial cells of the retina. Their end-feet form the limits of the retina at the outer and inner limiting membranes (ILM), and in conjunction with astrocytes, pericytes and endothelial cells they establish the blood-retinal barrier (BRB). BRB limits material transport between the bloodstream and the retina while the ILM acts as a basement membrane that defines histologically the border between the retina and the vitreous cavity. Labeling Müller cells is particularly relevant to study the physical state of the retinal barriers, as these cells are an integral part of the BRB and ILM. Both BRB and ILM are frequently altered in retinal disease and are responsible for disease symptoms.There are several well-established methods to study the integrity of the BRB, such as the Evans blue assay or fluorescein angiography. However these methods do not provide information on the extent of BRB permeability to larger molecules, in nanometer range. Furthermore, they do not provide information on the state of other retinal barriers such as the ILM. To study BRB permeability alongside retinal ILM, we used an AAV based method that provides information on permeability of BRB to larger molecules while indicating the state of the ILM and extracellular matrix proteins in disease states. Two AAV variants are useful for such study: AAV5 and ShH10. AAV5 has a natural tropism for photoreceptors but it cannot get across to the outer retina when administered into the vitreous when the ILM is intact (i.e., in wild-type retinas). ShH10 has a strong tropism towards glial cells and will selectively label Müller glia in both healthy and diseased retinas. ShH10 provides more efficient gene delivery in retinas where ILM is compromised. These viral tools coupled with immunohistochemistry and blood-DNA analysis shed light onto the state of retinal barriers in disease. 相似文献
12.
van Echten-Deckert G Saathoff M Kirfel G Herzog V 《European journal of cell biology》2007,86(11-12):675-682
During mammalian embryogenesis the emerging epidermis is temporarily covered by an epithelial monolayer, the periderm. In chicken, a second epithelial layer, the subperiderm, located underneath the periderm develops in later embryogenesis. Together the periderm and the subperiderm are referred to as the PSP unit. The cells of the PSP unit are tightly connected by tight junctions (TJ), thereby providing the embryo with an impermeable bilayered diffusion barrier. The emerging epidermis assumes its barrier function by cornification beginning at embryonic day 17 (E17) before at E18 the PSP unit undergoes desquamation. Lipid analysis of both epithelia after their mechanical separation revealed a dramatic increase to about 100-fold values of barrier-relevant ceramides, i.e. those known to essentially contribute to the diffusion barrier of the cornified envelope, in the emerging epidermis between E17 and E19. In contrast, the content of barrier-relevant ceramides in the PSP unit remained at constantly low levels throughout embryogenesis. These data strongly argue in favour of different mechanisms for the barrier function of the two epithelia. TJ in the PSP unit provide the main diffusion barrier protecting the embryo until beginning of desquamation at E18. At this developmental stage the content of cornified envelope-specific ceramides is substantially elevated, thus enabling the epidermis to fulfil its function as the major diffusion barrier after desquamation of the PSP unit. The observation that barrier-relevant ceramides are formed prior to desquamation of the PSP unit points to a precisely regulated sequence in that desquamation does not occur until the lipid-based barrier of the cornified envelope is completed and suggests in addition that these lipids might be essential regulators of the interaction between the PSP unit and the emerging epidermis. 相似文献
13.
Caroline Geeraerts Michaël Ovidio Hilde Verbiest David Buysse Johan Coeck Claude Belpaire Jean-Claude Philippart 《Hydrobiologia》2007,582(1):143-153
Adult roach Rutilus rutilus (L.) (N = 24; 19.9–36.1 cm FL) from three highly fragmented Belgian rivers were tagged with surgically implanted radio transmitters.
Their seasonal movements were observed from March to August 2004 (circum reproduction period) in river stretches delimited
by two physical barriers. In the three rivers, roach displayed similar patterns of movements which were mainly influenced
by the date of observation (movements increased in late April–May) and water temperature (travel distances were more important
when water temperature ranged between 10°C and 14°C). Roach sometimes cleared physical obstacles. The mean distances travelled
in each river were relatively short (max. 2.5 km) and mainly influenced by the length of the study reach, which was delimited
by physical barriers. 相似文献
14.
Using miniaturized radiotelemetry to discover the breeding grounds of the endangered New Zealand Storm Petrel Fregetta maoriana 下载免费PDF全文
Matt J. Rayner Chris P. Gaskin Neil B. Fitzgerald Karen A. Baird Martin M. Berg David Boyle Leigh Joyce Todd J. Landers Graeme G. Loh Sue Maturin Lyndon Perrimen R. Paul Scofield Joanna Simm Ian Southey Graeme A. Taylor Alan J. D. Tennyson Bruce C. Robertson Megan Young Richard Walle Stefanie M. H. Ismar 《Ibis》2015,157(4):754-766
Identification of breeding sites remains a critical step in species conservation, particularly in procellariiform seabirds whose threat status is of global concern. We designed and conducted an integrative radiotelemetry approach to uncover the breeding grounds of the critically endangered New Zealand Storm Petrel Fregetta maoriana (NZSP), a species considered extinct before its rediscovery in 2003. Solar‐powered automated radio receivers and hand‐held telemetry were used to detect the presence of birds on three island groups in the Hauraki Gulf near Auckland, New Zealand. At least 11 NZSP captured and radiotagged at sea were detected at night near Te Hauturu‐o‐Toi/Little Barrier Island with the detection of an incubating bird leading to the discovery of the first known breeding site for this species. In total, four NZSP breeding burrows were detected under mature forest canopy and three adult NZSP and two NZSP chicks were ringed. Telemetry data indicated NZSP showed strong moonlight avoidance behaviour over the breeding site, had incubation shifts of approximately 5 days and had a breeding season extending from February to June/July, a different season from other Procellariiformes in the region. Radiotelemetry, in combination with rigorously collected field data on species distribution, offers a valuable technique for locating breeding grounds of procellariiform seabirds and gaining insights into breeding biology while minimizing disturbance to sensitive species or damage to fragile habitat. Our study suggests an avenue for other breeding ground searches in one of the most threatened avian Orders, and highlights the general need for information on the location of breeding sites and understanding the breeding biology in data‐deficient birds. 相似文献
15.
16.
Chen C Lu XH Yan S Chai H Yao Q 《Biochemical and biophysical research communications》2005,335(3):874-882
HIV protease inhibitors (PIs) are often associated with metabolic and cardiovascular complications although they are effective anti-HIV drugs. In this study, we determined whether HIV PI ritonavir could increase endothelial permeability, one of the important mechanisms of vascular lesion formation. Human dermal microvascular endothelial cells (HMECs) treated with ritonavir showed a significant increase of endothelial permeability in a dose- and time-dependent manner assayed with a transwell system. Ritonavir significantly reduced the mRNA levels of tight junction proteins zonula occluden-1, occludin, and claudin-1 by 40-60% as compared to controls (P<0.05) by real-time PCR analysis. Protein levels of these tight junction molecules were also substantially reduced in the ritonavir-treated cells. In addition, HMECs treated with ritonavir (7.5, 15, and 30microM) showed a substantial increase of superoxide anion production by 10%, 32%, and 65%, respectively, as compared to controls. Antioxidants (EGCG and SeMet) effectively reduced ritonavir-induced endothelial permeability. Furthermore, ritonavir activated ERK1/2 (phosphorylation), but not P38 and JNK. Specific ERK1/2 inhibitor, PD89059, significantly abolished ritonavir-induced endothelial permeability by 92%. Thus, HIV PI ritonavir increases endothelial permeability, decreases levels of tight junction proteins, and increases superoxide anion production. ERK1/2 activation is involved in the signal transduction pathway of ritonavir-induced endothelial permeability. 相似文献
17.
基质金属蛋白酶是一组金属依赖性的蛋白内切酶家族,可对细胞外基质进行特异的降解,在生理和璃理过程中都发挥着重要作用。已有许多有关基质金属蛋白酶在中枢神经系统的作用的研究报道,本文对基质金属蛋白酶在脑缺血和脑出血等脑血管的急性损伤作用进行了综述,并对进一步研究方向作出展望。 相似文献
18.
Deposition and cycling of carbon and nitrogen in carbonate mud of the lagoons of Arlington and Sudbury Reefs, Great Barrier Reef 总被引:2,自引:2,他引:0
The dynamics of carbon and nitrogen in carbonate mud were examined in the lagoons of Arlington and Sudbury Reefs, Great Barrier Reef. Most (89–93%) of the organic carbon and total nitrogen depositing to the carbonate mud zones was mineralized over a sediment depth of 1 m, with ∼50% of CO2 produced during microbial decomposition involved in carbonate precipitation/dissolution reactions. There was proportionally little burial of organic carbon (10–11%) or nitrogen (7–10%). Nitrogen budgets suggest rapid turnover of porewater inorganic N pools on the order of hours to a few days. Incubation experiments indicate carbonate dissolution in surface deposits (≤20 cm depth) and carbonate precipitation in deeper sediments. Depth-integrated reaction rates indicate net carbonate precipitation of 7–10 mol CaCO3 m2 year−1 over a depth of 1 m. Budget calculations at the whole-reef scale imply that deposition of CaCO3 in the mud zones of both lagoons may equate to 50–90% of total reef carbonate production, with organic carbon fluxes equating to nearly all net primary production on each reef. These biogeochemical estimates point to the functional importance of carbonate mud zones in the lagoons of the shelf reefs of the Great Barrier Reef. 相似文献
19.
Jong A Wu CH Gonzales-Gomez I Kwon-Chung KJ Chang YC Tseng HK Cho WL Huang SH 《The Journal of biological chemistry》2012,287(19):15298-15306
Cryptococcus neoformans is a pathogenic yeast that can invade the brain and cause meningoencephalitis. Our previous in vitro studies suggested that the interaction between C. neoformans hyaluronic acid and human brain endothelial CD44 could be the initial step of brain invasion. In this report, we used a CD44 knock-out (KO or CD44(-/-)) mouse model to explore the importance of CD44 in C. neoformans brain invasion. Our results showed that C. neoformans-infected CD44 KO mice survived longer than the infected wild-type mice. Consistent with our in vitro results, the brain and cerebrospinal fluid fungal burden was reduced in CD44-deficient mice. Histopathological studies showed smaller and fewer cystic lesions in the brains of CD44 KO mice. Interestingly, the cystic lesions contained C. neoformans cells embedded within their polysaccharide capsule and were surrounded by host glial cells. We also found that a secondary hyaluronic acid receptor, RHAMM (receptor of hyaluronan-mediated motility), was present in the CD44 KO mice. Importantly, our studies demonstrated an in vivo blocking effect of simvastatin. These results suggest that the CD44 and RHAMM receptors function on membrane lipid rafts during invasion and that simvastatin may have a potential therapeutic role in C. neoformans infections of the brain. 相似文献
20.