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91.
RuvB family of protein contains two similar kinds of proteins i.e. RuvB1 and RuvB2 from yeast to human. These proteins belong to the AAA + class of proteins and are critical components of several multiprotein complexes involved in diverse cellular activities. There are two RuvB proteins annotated in the Plasmodium database but the identification of the third protein recently by our lab has raised the question why Plasmodium falciparum contains three RuvB proteins instead of two. Hence the biochemical characterizations of these proteins have become essential to understand the role of these proteins in the malaria parasite. Recently we have reported the characterization of the recombinant PfRuvB3, which contains ATPase activity but lacks DNA helicase activity. In the present study we report the phylogenetic analysis and detailed biochemical characterization of one of the other RuvB homologue RuvB1 from P. falciparum. PfRuvB1 shows considerable homology with human as well as yeast RuvB1 and contains Walker motif A and Walker motif B. The activity analysis of this protein revealed that PfRuvB1 is an ATPase and this activity increased significantly in the presence of ss-DNA. PfRuvB1 also contains DNA helicase activity and translocates preferentially in 5′ to 3′ direction. In vivo investigation of PfRuvB1 revealed that it is constitutively expressed during all the stages of intraerythrocytic cycle of P. falciparum and localizes mainly to the nucleus. These studies will make important contribution in understanding the role of RuvB protein in P. falciparum. 相似文献
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93.
Luís Roque Noélia Duarte Maria Rosário Bronze Catarina Garcia Julia Alopaeus Jesus Molpeceres 《Biofouling》2013,29(8):880-892
AbstractGlycyrrhiza glabra L. is considered an important source of bioactive compounds. This study aimed at the development of an efficient solution for the treatment of oral candidiasis. Several extracts of Glycyrrhiza glabra L. were prepared using different solvents and their potential in vitro antifungal activity was assessed. Ethanolic extracts showed the most promising results against C. albicans. This extract was incorporated into mucoadhesive nanoparticles (PLA, PLGA and alginate), which were further included in an oral gel, an oral film and a toothpaste, respectively. The results showed that nanoparticles were successfully produced, presenting a mean size among 100–900?nm with high encapsulation efficiency. In vitro studies showed that the most bioadhesive formulation was the oral film with extract-loaded PLGA nanoparticles, followed by the toothpaste with extract-loaded alginate nanoparticles and the oral gel with extract-loaded PLA nanoparticles. 相似文献
94.
Efrem Kentiba Soumitra Mondal D. Mathivanan Mala George 《Chronobiology international》2013,30(12):1742-1747
ABSTRACTThe purpose of this study was to compare chronotype preferences of college students from high- and low-altitude backgrounds living in a tropical setting of Ethiopia. Chronotype (morningness–eveningness) is a preference for a given time of day for physical or mental activities. The present cross-sectional study employed Horne and Osteberg Morningness-Eveningness Questionnaires to evaluate chronotype preferences. The chronotype preference of 264 male college students from varied altitude backgrounds indicated significant differences (p < 0.001). Our findings confirm our hypothesis, of the prevalence of M-types dominant chronotype among college students at low than high altitude. However, we did not confirm our second hypothesis, since students from high-altitude backgrounds were generally I-type dominant chronotype. Similarly, students’ academic performances from low- compared to high-altitudes backgrounds also indicated significant differences (p < 0.003). Better academic performances were seen in students with I-type chronotype orientations from high altitudes. 相似文献
95.
Thomas Soehl & Roger Waldinger 《Ethnic and racial studies》2013,36(9):1489-1510
This paper uses the Pew Hispanic Center's 2006 National Survey of Latinos to study the everyday, routine cross-border activities of travel, remittance sending and telephone communication among Latin American immigrants in the United States. We ask how migrants vary in the intensity of their cross-border connections, distinguishing among the transmigrants, those captured by the host-country national social field, and those who maintain some ongoing home-country tie. We then examine the characteristics associated both with variations in the intensity of connectedness and with each specific type of connection. We show that most migrants maintain some degree of home-country connectedness, with a minority severing ties and a still smaller minority maintaining ties at a high degree of intensity. Connectivity is highly responsive to the location of key social ties, acculturation, and citizenship status, as well as the costs associated with the different types of cross-border activity. 相似文献
96.
Camelia Algora Friederike Gründger Lorenz Adrian Volkmar Damm Hans-Hermann Richnow Martin Krüger 《Geomicrobiology journal》2013,30(8):690-705
The Northern Baffin Bay between Greenland and Canada is a remote Arctic area restricted in primary production by seasonal ice cover, with presumably low sedimentation rates, carbon content and microbial activities in its sediments. Our aim was to study the so far unknown subseafloor geochemistry and microbial populations driving seafloor ecosystems. Shelf sediments had the highest organic carbon content, numbers of Bacteria and Archaea, and microcosms inoculated from Shelf sediments showed highest sulfate reduction and methane production rates. Sediments in the central deep area and on the southern slope contained less organic carbon and overall lower microbial numbers. Similar 16S rRNA gene copy numbers of Archaea and Bacteria were found for the majority of the sites investigated. Sulfate in pore water correlated with dsrA copy numbers of sulfate-reducing prokaryotes and differed between sites. No methane was found as free gas in the sediments, and mcrA copy numbers of methanogenic Archaea were low. Methanogenic and sulfate-reducing cultures were enriched on a variety of substrates including hydrocarbons. In summary, the Greenlandic shelf sediments contain vital microbial communities adapted to their specific environmental conditions. 相似文献
97.
Bin Yu Xiao-Nan Sun Xiao-Jing Shi Ping-Ping Qi Yuan Fang En Zhang De-Quan Yu Hong-Min Liu 《Steroids》2013,78(11):1134-1140
The stereoselective and metal-free protocol involving a cascade aldol/cyclization process for the synthesis of steroidal (E, E) dienamides from steroidal α, α-dicyanoalkene was reported. This protocol efficiently achieved the construction of CC bond and selective conversion of cyano group into carboxamide in one-pot procedure under mild condition. Further biological evaluation showed that some of these compounds had moderate to excellent cytotoxic activities against all the tested cancer cell lines and were more potent than well-known drug 5-fluorouracil. Particularly, compound 3c represented excellent inhibitory effect against MCF-7 (IC50 = 0.76 μM), which was about 10-fold more potent than 5-fluorouracil. 相似文献
98.
Feng Jun-Tao Wang De-Long Wu Yong-Ling Yan He Zhang Xing 《Bioorganic & medicinal chemistry letters》2013,23(15):4393-4397
Thirty new and thirty-four known analogues were designed and synthesized to improve the potential use of the α-methylene-γ-butyrolactone ring, a natural pharmacophore. All structures were confirmed by 1H and 13C NMR, MS, and single-crystal X-ray diffraction analyses. The results of antifungal and cytotoxic activity indicated that the synthesized analogues showed significant inhibitory activity and limited selectivity. Compound 45 exhibited the highest antifungal activity with IC50 = 22.8 μM but moderate cytotoxic activity with IC50 = 28.5 μM (against BGC823 cell line) and 7.7 μM (against HeLa cell line). Analysis of structure–activity relationships revealed that the incorporation of an aromatic ring into the β, γ positions of the lactone ring improved antifungal activity, and that the introduction of electron-withdrawing groups into the aromatic rings increased the activity compared with electron-donating groups. The above results identified 4-phenyl-3-phenyl-2-methylenebutyrolactone (33) as a lead scaffold for discovering and developing novel and improved crop-protection agents. 相似文献
99.
Hua Chen Shuai Li Yuchao Yao Likai Zhou Jianpeng Zhao Yunjing Gu Kerang Wang Xiaoliu Li 《Bioorganic & medicinal chemistry letters》2013,23(17):4785-4789
Novel triphenylethylene–coumarin hybrid derivatives containing different amounts of amino side chains were designed and synthesized in good yields under microwave radiation. The derivatives 5b–d which possessed two amino side chains (except morpholinyl) showed a broad-spectrum and good anti-proliferative activity against five tumor cells and low cytotoxicity in osteoblast. UV–vis, fluorescence, and circular dichroism (CD) spectroscopies and thermal denaturation exhibited that compounds 10c, 5c, and 13c bearing amino side chain (except morpholinyl) on 4-phenyl had significant interactions with Ct-DNA by the intercalative mode of binding. Structure–activity relationships (SARs) analysis suggested that the amino alkyl chain would play an important role both in the compounds against tumor cells proliferation and their interactions with DNA. 相似文献
100.
人工管理和自然驱动下盐城海滨湿地互花米草沼泽演变及空间差异 总被引:2,自引:0,他引:2
将盐城国家级自然保护区核心区划分为人工管理区和自然湿地区两种模式,根据2000年、2006年、2011年3个时相的ETM+影像,运用RS、GIS技术和景观生态学方法,分析不同驱动力下互花米草沼泽景观变化的差异.结果表明:(1)人工管理区,2000-2011年,互花米草沼泽面积由272hm2增加到781hm2;平均分维数呈下降的趋势,聚合度指数呈上升的趋势,表明互花米草沼泽在空间上趋于聚集,在形状上趋于规则;从互花米草沼泽增长速率看,2000-2011年,呈现先快后慢的特征.在景观空间演变上,互花米草沼泽的平均宽度从598.679m增加到1719.002m,表现出向海陆两个方向扩张的特征.(2)自然湿地区,2000-2011年互花米草沼泽面积由2023hm2增加到3974hm2;平均分维数和聚合度指数呈先上升后下降的趋势;米草沼泽增长稳定,年增长约160-180 hm2/a,单位长度岸线年增长面积约为15 hm2· km-1·a-1左右;比较发现,自然湿地区互花米草沼泽的增长速度高于人工管理区的增长速度.在景观空间演变上,互花米草沼泽平均宽度从1625.586m增加到3193.317m,2000-2006年表现出向海洋单方向扩张的特征,2006-2011年表现出向海陆两个方向扩张的性质.(3)在自然条件下互花米草沼泽扩张受到气候、地貌过程、水文过程、植被类型及种间竞争的影响,表现出明显的带状特征.在人工管理下,通过建设拦水堤坝等,阻止了潮汐作用下海水的扩散能力,生态过程发生改变,致使生态系统类型向淡水湿地方向演变,抑制了互花米草沼泽的扩张,表现出一定的镶嵌格局. 相似文献