排序方式: 共有89条查询结果,搜索用时 156 毫秒
81.
Reuter A Fortunato D Garoffo LP Napolitano L Scheurer S Giuffrida MG Vieths S Conti A 《Proteomics》2005,5(1):282-289
Birch pollen-related food allergies are mainly associated to Bet v 1. Little is known about isoforms of Bet v 1 homologous in fruit of the Rosaceae family. We attempted to identify novel isoforms of Pru av 1, the major cherry allergen, at the cDNA and the protein level by a combination of molecular biology and proteomic tools. A cDNA library was screened with patients immunoglobulin E (IgE) and a specific hybridization probe. Edman sequencing, mass spectrometry (MS), and MS/MS were performed after detecting Pru av 1 on 2-D maps by immunoblotting using patients IgE and a monoclonal antibody. Partial amino acid sequences were completed with a polymerase chain reaction (PCR) strategy. The IgE-binding properties of the Pru av 1 spots were analyzed by 2-D blot inhibition. cDNA library analysis revealed a novel Pru av 1 isoform. MS and N-terminal sequencing confirmed the cDNA sequences at the protein level. A series of spots were confirmed as the already known Pru av 1. One spot, exclusively detected with patients sera, was identified as the novel isoform. A partial amino acid sequence detected with MS/MS was completed by PCR-cloning. The 2-D blot inhibition revealed epitope differences between the novel isoform and the previously published Pru av 1. Our data demonstrate that a synergistic combination of molecular biology and proteomics represents a powerful tool for reliable and comprehensive identification of allergen isoforms and variants. The newly identified isoform showed diverging IgE-binding properties and may be relevant for the diagnosis or therapy of cherry allergy. 相似文献
82.
Carsten Schleh Barbara M Rothen-Rutishauser Fabian Blank Hans D Lauenstein Matthias Nassimi Norbert Krug Armin Braun Veit J Erpenbeck Peter Gehr Jens M Hohlfeld 《Respiratory research》2012,13(1):1-10
Background
The obese-asthma phenotype is not well defined. The aim of this study was to examine both mechanical and inflammatory influences, by comparing lung function with body composition and airway inflammation in overweight and obese asthma.Methods
Overweight and obese (BMI 28-40 kg/m2) adults with asthma (n = 44) completed lung function assessment and underwent full-body dual energy x-ray absorptiometry. Venous blood samples and induced sputum were analysed for inflammatory markers.Results
In females, android and thoracic fat tissue and total body lean tissue were inversely correlated with expiratory reserve volume (ERV). Conversely in males, fat tissue was not correlated with lung function, however there was a positive association between android and thoracic lean tissue and ERV. Lower body (gynoid and leg) lean tissue was positively associated with sputum %neutrophils in females, while leptin was positively associated with android and thoracic fat tissue in males.Conclusions
This study suggests that both body composition and inflammation independently affect lung function, with distinct differences between males and females. Lean tissue exacerbates the obese-asthma phenotype in females and the mechanism responsible for this finding warrants further investigation. 相似文献83.
Stefan Kofler Chloé Ackaert Martin Samonig Claudia Asam Peter Briza Jutta Horejs-Hoeck Chiara Cabrele Fatima Ferreira Albert Duschl Christian Huber Hans Brandstetter 《The Journal of biological chemistry》2014,289(1):540-551
Many allergens share several biophysical characteristics, including the capability to undergo oligomerization. The dimerization mechanism in Bet v 1 and its allergenic properties are so far poorly understood. Here, we report crystal structures of dimeric Bet v 1, revealing a noncanonical incorporation of cysteine at position 5 instead of genetically encoded tyrosine. Cysteine polysulfide bridging stabilized different dimeric assemblies, depending on the polysulfide linker length. These dimers represent quaternary arrangements that are frequently observed in related proteins, reflecting their prevalence in unmodified Bet v 1. These conclusions were corroborated by characteristic immunologic properties of monomeric and dimeric allergen variants. Hereby, residue 5 could be identified as an allergenic hot spot in Bet v 1. The presented results refine fundamental principles in protein chemistry and emphasize the importance of protein modifications in understanding the molecular basis of allergenicity. 相似文献
84.
Dessislava Georgieva Nicolay Genov 《Biochemical and biophysical research communications》2009,378(4):711-715
The acid phosphatase Api m 3 is the major allergen of the honeybee venom. Except for the amino acid sequence, no other structural information for the enzyme is available. We applied homology modeling to assign the three-dimensional structure of Api m 3. The structure of the homodimeric human prostatic acid phosphatase was used to model the Api m 3 tertiary structure. IgE epitopes and antigenic sites were predicted using programs based on the structure of known epitopes and analysis of the 3-D model. The model of Api m 3 revealed an active site similar to those of the histidine—type acid phosphatases with conservation of the catalytically important residues. The observed substitutions in the phosphate ion binding site suggest differences in the substrate specificity in comparison to other acid phosphatases. The analysis of the Api m 3 three-dimensional model revealed a very likely mechanism of enzyme action. 相似文献
85.
Goro Ishikawa Yoshitake Takada Toshiki Nakamura 《Molecular breeding : new strategies in plant improvement》2006,17(4):365-374
The soybean cultivar Yumeminori, which lacks the α′- and α-subunits of β-conglycinin, carries both naturally occurring and induced mutations. While the cause of the natural mutation resulting in the α′-subunit deficiency has been determined, the induced mutation in the CG-2 gene encoding the α-subunit has not been characterized at the molecular level. In this study, we identified a four base pair insertion in the first exon of CG-2, which introduced a premature stop codon. The insertion co-segregated with the lack of α-subunit, indicating that this mutation is the cause of the α-subunit deficiency. A multiplex PCR method of testing for the presence or absence of α′- and α-subunits was developed based on the sequences of mutated and wild-type alleles. This PCR-based test was also capable of detecting the presence of wild-type genes when Yumeminori DNA samples were contaminated with wild-type DNA at levels of 0.2% or greater. Thus, this method will be useful both for marker-assisted selection in soybean breeding programs, and for seed purity tests in food industries. 相似文献
86.
Yano H Sugihara Y Shirai H Wagatsuma Y Kusada O Matsuda T Kuroda S Higaki S 《Amino acids》2006,30(3):303-305
Summary. Phthalocyanine (Pc)-dyed fiber is reported to reduce atopic symptoms in some patients when they use underwear made of the
fiber. We investigated the adsorption of allergens on Pc-fiber. Pc-fiber trapped house dust/pollen/food allergens with varied
molecular weight and pI. The adsorbed allergens were released in the presence of mild detergent. Pc-fiber did not change the
molecular weight or disulfide bonding of the allergens. These observations imply that Pc-fiber is applicable as an “allergen
trap” for a wide variety of products. 相似文献
87.
During the past few decades, house dust mites have attracted worldwide interest among medical entomologists and acarologists
because of their importance in causing nasobronchial allergic disorders in human beings. House dust mites are present throughout
the year; however, their relative densities differ in different seasons and habitats. Because the prevalence of house dust
mite allergen is important epidemiologically and clinically, detailed knowledge on the seasonal abundance of important allergenic
mites is of great importance for better understanding of the pathogenesis of the disease. In view of this, a systematic survey
was carried out on the prevalence of total mites and four common allergenic mites in the city of Kolkata for two consecutive
years. Both bed and bedroom floor dust were collected separately from homes inhabited by asthmatic patients situated in different
corners of the city on monthly basis from January 2004 to December 2005. The population levels of total mites and four common
allergenic mites, namely Dermatophagoides pteronyssinus, D. farinae, Austroglycyphagus geniculatus, and Blomia tropicalis separately, were highest during the pre-monsoon period (March–May), irrespective of habitat, whereas densities were low in
all cases during winter (December–February). The study indicates that season had the most significant effect on the relative
abundance of house dust mites except Dermatophagoides farinae, irrespective of habitat. 相似文献
88.
Hideki Nagata Atsuo Amano Takashi Hanioka Hiroo Tamagawa Satoshi Shizukuishi Toshiyuki Miyata 《FEMS microbiology letters》1993,114(1):31-36
Abstract The aspfI gene encoding a ribonucleotoxin, a putative virulence factor of Aspergillus fumigatus , was inactivated by gene disruption. Gene replacement through homologous recombination by the disrupted allele tagged by the hygromycin B resistance marker was performed by transformation of a pathogenic strain. One transformant with the disrupted aspfI gene failed to produce the ASPFI protein and was shown to be pathogenic for mice. We concluded that this ribotoxin is not a main factor in the colonization of the lung tissues by A. fumigatus . 相似文献
89.