全文获取类型
收费全文 | 444篇 |
免费 | 48篇 |
国内免费 | 8篇 |
出版年
2023年 | 7篇 |
2022年 | 1篇 |
2021年 | 5篇 |
2020年 | 22篇 |
2019年 | 22篇 |
2018年 | 15篇 |
2017年 | 18篇 |
2016年 | 26篇 |
2015年 | 16篇 |
2014年 | 26篇 |
2013年 | 21篇 |
2012年 | 15篇 |
2011年 | 13篇 |
2010年 | 25篇 |
2009年 | 21篇 |
2008年 | 34篇 |
2007年 | 31篇 |
2006年 | 30篇 |
2005年 | 16篇 |
2004年 | 21篇 |
2003年 | 10篇 |
2002年 | 21篇 |
2001年 | 12篇 |
2000年 | 5篇 |
1999年 | 8篇 |
1998年 | 13篇 |
1997年 | 10篇 |
1996年 | 8篇 |
1995年 | 2篇 |
1994年 | 3篇 |
1993年 | 1篇 |
1992年 | 2篇 |
1991年 | 1篇 |
1989年 | 1篇 |
1988年 | 1篇 |
1987年 | 1篇 |
1986年 | 2篇 |
1985年 | 3篇 |
1984年 | 2篇 |
1982年 | 2篇 |
1981年 | 3篇 |
1978年 | 2篇 |
1975年 | 1篇 |
1973年 | 1篇 |
排序方式: 共有500条查询结果,搜索用时 15 毫秒
71.
Lorenzo Alibardi 《Journal of morphology》2020,281(1):68-80
A highly upregulated gene during tail regeneration in lizards is Wnt2b, a gene broadly expressed during development. The present study examines the distribution of Wnt proteins, most likely wnt2b, by western blotting and immunofluorescence in the blastema-cone of lizards using a specific antibody produced against a lizard Wnt2b protein. Immunopositive bands at 48–50 and 18 kDa are present in the regenerative blastema, the latter likely as a degradation product. Immunofluorescence is mainly observed in the wound epidermis, including in the Apical Epidermal Peg where the protein appears localized in intermediate and differentiating keratinocytes. Labeling is more intense along the perimeter of keratinocytes, possibly as a secretory product, and indicates that the high epidermal proliferation of the regenerating epidermis is sustained by Wnt proteins. The regenerating spinal cord forms an ependymal tube within the blastema and shows immunolabeling especially in the cytoplasm of ependymal cells contacting the central canal where some secretion might occur. Also, regenerating nerves and proximal spinal ganglia innervating the regenerating blastema contain this signaling protein. In contrast, the blastema mesenchyme, muscles and cartilage show weak immunolabeling that tends to disappear in tissues located in more proximal regions, close to the original tail. However, a distal to proximal gradient of Wnt proteins was not detected. The present study supports the hypothesis that Wnt proteins, in particular Wnt2b, are secreted by the apical epidermis covering the blastema and released into the mesenchyme where they stimulate cell multiplication. 相似文献
72.
73.
74.
《Saudi Journal of Biological Sciences》2017,24(4):966-971
We report morphometric data for 133 specimens of Diplometopon zarudnyi (Squamata: Trogonophidae) collected across its range within Riyadh province of Saudi Arabia. One-way analyses of variance revealed that differences exist in most characters. Snout-vent length and vent-tail length showed slight and inconsistent differences among samples. D. zarudnyi is characterized by 164–175 body annuli; 165–178 dorsal annuli; 13–17 caudal annuli with absence of caudal autotomy. In addition, 2–4 lateral annuli; 45–54 mid-body segments; 39–50 posterior segments; 4–5 head’s plates and 4–6 pre-cloacal pores were recorded in both males and females without gender difference. The present study revealed the widespread distribution of this species in the studied region, since land topography in this area is characterized by the absence of any natural barriers which could restrict the spread of this amphisbaenian lizard D. zarudnyi. 相似文献
75.
76.
Origins and patterns of endemic diversity in two specialized lizard lineages from the Australian Monsoonal Tropics (Oedura spp.) 下载免费PDF全文
Aim
Savanna biomes cover around 20% of land surfaces, yet the origins and processes that have shaped their biodiversity remain understudied. Here, we assess the timing of diversification and how patterns of genetic diversity vary along an aridity gradient in specialized saxicoline gecko clades (Oedura spp.) from the tropical savannas of northern Australia.Location
Australian Monsoonal Tropics (AMT), Kimberley region (Western Australia).Methods
We compiled mitochondrial and nuclear data for two Kimberley endemic lizard clades (Oedura filicipoda/murrumanu and O. gracilis), and allied non‐Kimberley taxa (O. marmorata complex). Species delimitation methods were used to identify evolutionary lineages, Maximum‐likelihood and Bayesian phylogenetic methods were employed to assess relationships and diversification timeframes, and rainfall data and range sizes were tested for correlations.Results
Phylogenetic analyses of cryptic or recently discovered lineage diversity revealed late‐Miocene to early‐Pliocene crown ages. Microendemism and diversity were highest in high‐rainfall regions, while the most widespread lineages occurred in the central and south‐east Kimberley, and showed evidence of introgression with parapatric lineages.Main conclusions
The initial diversification in both clades was broadly concordant with global climatic events linked to the expansion of savanna biomes in the lateMiocene. Higher endemism in mesic and refugial areas suggests long histories of localized persistence, while wider distributions and evidence of introgression suggest a dynamic history at the arid‐monsoonal interface. 相似文献77.
S. V. Nielsen J. L. Banks R. E. Diaz Jr P. A. Trainor T. Gamble 《Journal of evolutionary biology》2018,31(4):484-490
Much of our current state of knowledge concerning sex chromosome evolution is based on a handful of ‘exceptional’ taxa with heteromorphic sex chromosomes. However, classifying the sex chromosome systems of additional species lacking easily identifiable, heteromorphic sex chromosomes is indispensable if we wish to fully understand the genesis, degeneration and turnover of vertebrate sex chromosomes. Squamate reptiles (lizards and snakes) are a potential model clade for studying sex chromosome evolution as they exhibit a suite of sex‐determining modes yet most species lack heteromorphic sex chromosomes. Only three (of 203) chameleon species have identified sex chromosome systems (all with female heterogamety, ZZ/ZW). This study uses a recently developed method to identify sex‐specific genetic markers from restriction site‐associated DNA sequence (RADseq) data, which enables the identification of sex chromosome systems in species lacking heteromorphic sex chromosomes. We used RADseq and subsequent PCR validation to identify an XX/XY sex chromosome system in the veiled chameleon (Chamaeleo calyptratus), revealing a novel transition in sex chromosome systems within the Chamaeleonidae. The sex‐specific genetic markers identified here will be essential in research focused on sex‐specific, comparative, functional and developmental evolutionary questions, further promoting C. calyptratus’ utility as an emerging model organism. 相似文献
78.
79.
Kenneth I. Warheit Jonathan D. Forman Jonathan B. Losos Donald B. Miles 《Evolution; international journal of organic evolution》1999,53(4):1226-1234
We compared the morphological diversity (i.e., the amount of morphological space occupied) of two similar clades, the lizard genera Anolis and Sceloporus. These species-rich monophyletic clades are similar in body size, age of origin, and many aspects of their natural history. We examined a number of morphological traits whose variation is likely to represent adaptation to different aspects of the environment, including body size, limb proportions, head dimensions, and tail length. Examination of the position of species in multidimensional space, based on a principal components analysis, indicates that the morphological diversity of Anolis, which we refer to as disparity, is significantly greater than that of Sceloporus. One potential explanation for this pattern is that morphological diversification in Anolis was facilitated by the evolution of subdigital toe-pads, which allow anoles to use the environment in ways not available to Sceloporus. The geographic location of diversification (tropical and subtropical for Anolis, arid for Sceloporus) may also have been important. 相似文献
80.
The mitochondrial genome of the Komodo dragon (Varanus komodoensis) was nearly completely sequenced, except for two highly repetitive noncoding regions. An efficient sequencing method for squamate mitochondrial genomes was established by combining the long polymerase chain reaction (PCR) technology and a set of reptile-oriented primers designed for nested PCR amplifications. It was found that the mitochondrial genome had novel gene arrangements in which genes from NADH dehydrogenase subunit 6 to proline tRNA were extensively shuffled with duplicate control regions. These control regions had 99% sequence similarity over 700 bp. Although snake mitochondrial genomes are also known to possess duplicate control regions with nearly identical sequences, the location of the second control region suggested independent occurrence of the duplication on lineages leading to snakes and the Komodo dragon. Another feature of the mitochondrial genome of the Komodo dragon was the considerable number of tandem repeats, including sequences with a strong secondary structure, as a possible site for the slipped-strand mispairing in replication. These observations are consistent with hypotheses that tandem duplications via the slipped-strand mispairing may induce mitochondrial gene rearrangements and may serve to maintain similar copies of the control region. 相似文献