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排序方式: 共有268条查询结果,搜索用时 15 毫秒
261.
262.
263.
《Biochimica et Biophysica Acta (BBA)/General Subjects》1982,714(2):257-264
A cyclic nucleotide-independent protein kinase of human platelets, which phosphorylated histones, myelin basic protein and protamine and did not catalyze the phosphorylation of acidic proteins such as casein, phosvitin and myosin light chain, has been purified approx. 1,500-fold from the crude extract by steps of DEAE-cellulose, Sephadex G-200, hydroxylapatite and phosphoryl cellulose column chromatography. The substrate phosphorylation by this kinase was markedly enhanced by calmodulin even in the absence of Ca2+, when mixed histone was used as a substrate. The interaction of the kinase with mixed histone resulted in an irreversible inactivation of the enzyme. Calmodulin prevented this inactivation, and this compound produced an apparent increase in histone phosphorylation by the kinase. It should be noted that acidic polypeptides such as troponin-C, phospholipids and nucleic acids have a similar ability. The addition of Ca2+ reduced the effect of calmodulin more than the effects of other acidic compounds. 相似文献
264.
Grace Y. Sun Hsueh-Meei Huang Renuka Chandrasekhar Ding Z. Lee Albert Y. Sun 《Journal of neurochemistry》1987,48(3):974-980
Alterations in brain phospholipid metabolism were observed after chronic ethanol administration for 16 days to developing rats. Animals were injected intraperitoneally with 32Pi 16 h prior to killing. Overall uptake of 32Pi by brain did not differ between the control and ethanol-treated groups, which were killed 2 h and 24 h after the last ethanol feeding. Except for an increase in the labeling of myelin after ethanol treatment, the amount of radioactivity recovered in the synaptosomal-mitochondrial and plasma membrane fractions of control and ethanol-treated groups was not different. Relative to the radioactivity of phosphatidylcholines, which indicated no change, there were increases (20-44%) in labeling of ethanolamine plasmalogens, phosphatidic acids, and phosphatidylinositols in cortical synaptosomes from the 2-h ethanol-treated group. In the plasma membrane fractions, however, increases (9-14%) in labeling of phosphatidylserines and phosphatidylinositols were observed in both 2- and 24-h ethanol-treated groups. In both membrane fractions, there was an obvious increase (44-86%) in labeling of polyphosphoinositides at 24 h after withdrawal from ethanol. Results thus indicate an adaptive increase in the biosynthesis of ethanolamine plasmalogen and brain acidic phospholipids due to chronic ethanol administration. Furthermore, the increase in labeling of polyphosphoinositides in the 24-h withdrawal group may reflect the hypoactivity associated with ethanol withdrawal. 相似文献
265.
Yasunori Inoue Keiko Yamaoka Kyoko Kimura Kaori Sawai Takashi Arai 《Journal of plant research》2000,113(1):39-44
Received 13 August 1999/ Accepted in revised form 22 December 1999 相似文献
266.
Role of phospholipid in the low affinity reactions between cytochrome c and cytochrome oxidase 总被引:1,自引:0,他引:1
The steady-state oxidation of ferrocytochrome c by cytochrome oxidase monitored spectrophotometrically showed that: (1) the kinetics were strictly biphasic with purified enzyme, while mitochondrial membrane-bound enzyme exhibited multiphasic kinetics with extended low affinity phases; (2) the TNmax for the highest affinity phase was as slow as 5-10 electron X s-1 for both preparations, while for the low affinity phases it was about 45 electron X s-1 for the purified enzyme and 150 electron X s-1 for the mitochondrial membrane-bound enzyme; (3) reconstitution of purified enzyme into acidic phospholipid vesicles partially repleted the extended low affinity phases, while reconstitution into uncharged vesicles had no effect. 相似文献
267.
Johan Thyberg Karin Blomgren 《Virchows Archiv. B, Cell pathology including molecular pathology》1990,59(1):1-10
The transition of adult rat aortic smooth muscle cells from a contractile to a synthetic phenotype during the first week of primary culture on a substrate of fibronectin in serum-free medium was studied by light and electron microscopy. The weak base chloroquine and the carboxylic ionophore monensin were both found to inhibit the spreading of the cells and the accompanying changes in cellular fine structure. The exchange of myofilament bundles for a prominent rough endoplasmic reticulum and Golgi complex was delayed and vacuoles filled with incompetely degraded material accumulated in the cytoplasm. The microtubule-disruptive drugs colchicine and nocodazole likewise opposed the spreading and fine structural reorganization of the cells. Most typically, the Golgi stacks were small and widely dispersed. In addition, vacuoles of the type mentioned above increased in number. On the other hand, there was surprisingly little effect of cytochalasin B, a drug that is supposed to interfere with the assembly of actin filaments. The observations suggest that the phenotypic modulation of arterial smooth muscle cells is dependent on: (a) lysosomal degradation of discarded cellular constituents, (b) active vesicular transport along the exocytic pathway to provide the expanding cell surface with new membrane, and (c) a normal microtubular cytoskeleton to ensure the establishment of a new and functionally efficient intracellular organization. 相似文献
268.
This research demonstrates that a leaf’s response to acid mist is dependent on the integrity of the leaf cuticle and that
significant differences in the structural and physiological disturbances in leaves can be attributable to different types
of wind action. Betula pubescens Ehrh. plants were located at adjacent, but contrasting, sites to create different wind treatments: (i) direct wind action,
(ii) indirect wind action and (iii) shelter from wind action (control). In combination with the wind treatments, acidic (pHs 5
and 3) or neutral (pH 7) mists were applied weekly. Wind action significantly increased visible leaf injury, microscopic cuticular
lesions and cuticular conductance (g
c
), but reduced photosynthetic rate (P
N
) and stomatal conductance (g
s
) compared to shelter. Wind action combined with acid mist was more injurious than wind action alone, but leaves sheltered
from wind action were highly resistant to the damaging effects of acid mist. Direct wind action combined with pH 3 mist resulted
in the highest values of g
c
and the greatest number of cuticular lesions. By contrast, indirect wind action combined with pH 3 mist induced most visible
injury, but relatively low values of g
c
and few microscopic cuticular lesions. Acid mist reduced P
N
only when leaves had been damaged by wind action. Higher values of g
c
were associated both with increases in the area of visible leaf injury and with the number of cuticular lesions. Compensatory
increase in P
N
of healthy tissue was evident in leaves exposed to combinations of wind action and acid mist.
Received: 10 November 1997 / Accepted: 6 March 1998 相似文献