全文获取类型
收费全文 | 610篇 |
免费 | 89篇 |
国内免费 | 16篇 |
出版年
2024年 | 2篇 |
2023年 | 10篇 |
2022年 | 12篇 |
2021年 | 43篇 |
2020年 | 38篇 |
2019年 | 46篇 |
2018年 | 33篇 |
2017年 | 42篇 |
2016年 | 24篇 |
2015年 | 25篇 |
2014年 | 69篇 |
2013年 | 56篇 |
2012年 | 32篇 |
2011年 | 37篇 |
2010年 | 21篇 |
2009年 | 16篇 |
2008年 | 29篇 |
2007年 | 25篇 |
2006年 | 26篇 |
2005年 | 12篇 |
2004年 | 9篇 |
2003年 | 8篇 |
2002年 | 7篇 |
2001年 | 4篇 |
2000年 | 9篇 |
1999年 | 10篇 |
1998年 | 7篇 |
1997年 | 5篇 |
1996年 | 6篇 |
1995年 | 4篇 |
1994年 | 3篇 |
1993年 | 5篇 |
1992年 | 3篇 |
1991年 | 6篇 |
1990年 | 1篇 |
1989年 | 1篇 |
1988年 | 2篇 |
1986年 | 3篇 |
1985年 | 2篇 |
1984年 | 3篇 |
1983年 | 3篇 |
1982年 | 2篇 |
1981年 | 2篇 |
1980年 | 4篇 |
1979年 | 4篇 |
1973年 | 3篇 |
1972年 | 1篇 |
排序方式: 共有715条查询结果,搜索用时 31 毫秒
51.
Licochalcone A (LA), a chalcone derived from liquorice, exhibits multiple biological activities, including anti-oxidation and anti-inflammation. This study aimed to investigate the role and underlying mechanism of LA in the abdominal aortic aneurysm (AAA). AAA model was established by continuous infusion of 1000 ng/kg/min of angiotensin II (AngII) in ApoE -/- mice for 4 weeks. At 7 days before AngII administration, 5 mg/kg/day or 10 mg/kg/day of LA was intraperitoneally administered to mice and continued for 4 weeks. The characteristics and quantification of AAAs were determined in situ. Real-time PCR or western blot was used to measure mRNA or protein levels of matrix metalloproteinase 2 and matrix metalloproteinase 9; pro-inflammatory cytokines tumor necrosis factor-α, interleukin-1β, and interleukin-6; apoptosis-related proteins Bax, Bcl-2, and active caspase-3; miR-181b; Sirtuin 1 (SIRT1); and heme oxygenase-1 (HO-1). Mouse-aorta-origin vascular smooth muscle (MOVAS) cells were used to confirm the involved pathways in vitro. We found LA administration dose-dependently reduced the incidence of AngII-induced AAA, aneurysm diameter enlargement, elastin degradation, matrix metalloproteinase production, pro-inflammatory cytokines and miR-181b expression, and vascular smooth muscle cell apoptosis. It elevated SIRT1 and HO-1 expression that was suppressed by AngII. AngII enhanced miR-181b but reduced SIRT1 and HO-1 expression in MOVAS cells. In AngII-stimulated MOVAS cells, downregulation of miR-181b significantly upregulated the expression of SIRT1 and HO-1, the effect of which was abrogated by SIRT1 siRNA. Collectively, LA could attenuate AngII-induced AAA by modulating the miR-181b/SIRT1/HO-1 signaling. LA might be a potential medical therapy for small AAA. 相似文献
52.
53.
Jia Fan Wenyong Fan Jianzhen Lei Yingying Zhou Hongfei Xu Isha Kapoor Guoqing Zhu Juejin Wang 《生物化学与生物物理学报:疾病的分子基础》2019,1865(1):218-229
Pressure overload-induced cardiac hypertrophy occurs in response to chronic blood pressure increase, and dysfunction of CaV1.2 calcium channel involves in cardiac hypertrophic processes by perturbing intracellular calcium concentration ([Ca2+]i) and calcium-dependent signaling. As a carbohydrate-binding protein, galectin-1 (Gal-1) is found to bind with CaV1.2 channel, which regulates vascular CaV1.2 channel functions and blood pressure. However, the potential roles of Gal-1 in cardiac CaV1.2 channel (CaV1.2CM) and cardiomyocyte hypertrophy remain elusive. By whole-cell patch clamp, we find Gal-1 decreases the ICa,L with or without isoproterenol (ISO) application by reducing the channel membrane expression in neonatal rat ventricular myocytes (NRVMs). Moreover, Gal-1 could inhibit the current densities of CaV1.2CM by an alternative exon 9*-dependent manner in heterologously expressed HEK293 cells. Of significance, overexpression of Gal-1 diminishes ISO or KCl-induced [Ca2+]i elevation and attenuates ISO-induced hypertrophy in NRVMs. Mechanistically, Gal-1 decreases the ISO or Bay K8644-induced phosphorylation of intracellular calcium-dependent signaling proteins δCaMKII and HDAC4, and inhibits ISO-triggered translocation of HDAC4 in NRVMs. Pathologically, we observe that the expressions of Gal-1 and CaV1.2E9* channels are synchronously increased in rat hypertrophic cardiomyocytes and hearts. Taken together, our study indicates that Gal-1 reduces the channel membrane expression to inhibit the currents of CaV1.2CM in a splice-variant specific manner, which diminishes [Ca2+]i elevation, and attenuates cardiomyocyte hypertrophy by inhibiting the phosphorylation of δCaMKII and HDAC4. Furthermore, our work suggests that dysregulated Gal-1 and CaV1.2 alternative exon 9* might be attributed to the pathological processes of cardiac hypertrophy, and provides a potential anti-hypertrophic target in the heart. 相似文献
54.
Cecilia Nigro Alessia Leone Michele Longo Immacolata Prevenzano Thomas H. Fleming Antonella Nicolò Luca Parrillo Rosa Spinelli Pietro Formisano Peter P. Nawroth Francesco Beguinot Claudia Miele 《生物化学与生物物理学报:疾病的分子基础》2019,1865(1):73-85
Impaired angiogenesis leads to long-term complications and is a major contributor of the high morbidity in patients with Diabetes Mellitus (DM). Methylglyoxal (MGO) is a glycolysis byproduct that accumulates in DM and is detoxified by the Glyoxalase 1 (Glo1). Several studies suggest that MGO contributes to vascular complications through mechanisms that remain to be elucidated. In this study we have clarified for the first time the molecular mechanism involved in the impairment of angiogenesis induced by MGO accumulation.Angiogenesis was evaluated in mouse aortic endothelial cells isolated from Glo1-knockdown mice (Glo1KD MAECs) and their wild-type littermates (WT MAECs). Reduction in Glo1 expression led to an accumulation of MGO and MGO-modified proteins and impaired angiogenesis of Glo1KD MAECs. Both mRNA and protein levels of the anti-angiogenic HoxA5 gene were increased in Glo1KD MAECs and its silencing improved both their migration and invasion. Nuclear NF-?B-p65 was increased 2.5-fold in the Glo1KD as compared to WT MAECs. Interestingly, NF-?B-p65 binding to HoxA5 promoter was also 2-fold higher in Glo1KD MAECs and positively regulated HoxA5 expression in MAECs. Consistent with these data, both the exposure to a chemical inhibitor of Glo1 “SpBrBzGSHCp2” (GI) and to exogenous MGO led to the impairment of migration and the increase of HoxA5 mRNA and NF-?B-p65 protein levels in microvascular mouse coronary endothelial cells (MCECs).This study demonstrates, for the first time, that MGO accumulation increases the antiangiogenic factor HoxA5 via NF-?B-p65, thereby impairing the angiogenic ability of endothelial cells. 相似文献
55.
Model studies of the flow in abdominal aortic aneurysms during resting and exercise conditions 总被引:3,自引:0,他引:3
Egelhoff CJ Budwig RS Elger DF Khraishi TA Johansen KH 《Journal of biomechanics》1999,32(12):980-1329
Pulsatile flow in abdominal aortic aneurysm (AAA) models has been examined in order to understand the hemodynamics that may contribute to growth of an AAA. The model studies were conducted by experiments (flow visualization and laser Doppler velocimetry) and by numerical simulation using physiologically realistic resting and exercise flow conditions. We characterize the flow for two AAA model shapes and sizes emulating early AAA development through moderate AAA growth (mean and peak Reynolds numbers of 362<Remean<1053 and 3308<Repeak<5696 with Womersley parameter 16.4<<21.2). The results of our investigation indicate that AAA flow can be divided into three flow regimes: (i) Attached flow over the entire cycle in small AAAs at resting conditions, (ii) vortex formation and translation in moderate size AAAs at resting conditions, and (iii) vortex formation, translation and turbulence in moderate size AAAs under exercise conditions. The second two regimes are classified in the medical literature as disturbed flow conditions that have been correlated with atherogenesis as well as thrombogenesis. Thus, AAA disturbed hemodynamics may be a contributing factor to AAA growth by accelerating the degeneration of the arterial wall. Our investigation also concluded that vortex development is considerably weaker in an asymmetric AAA. Furthermore, turbulence was not observed in the asymmetric model. Finally, our investigation suggests a new mode of transition to turbulence: vortex ring instability and bursting to turbulence. The transition process depends on a combination of the pulsatile flow conditions and the tube cross-sectional area change. 相似文献
56.
M. Kurokawa K. Kuwasawa S. Matsumura 《Journal of comparative physiology. A, Neuroethology, sensory, neural, and behavioral physiology》1999,185(1):11-19
Gill motor neuron L7-induced longitudinal shortening of the gill in Aplysia kurodai and A. juliana was suppressed when extracellular stimuli were applied to a restricted dorsal central region of the abdominal ganglion. We
found a neuron there which antagonized the L7-driven contraction. Since the contraction was suppressed when the identified
neuron was activated simultaneously with L7, we refer to the newly found neuron as “Anti-L7”. Anti-L7 did not change the L7
impulse generation in the abdominal ganglion. No direct synaptic connection from L7 to Anti-L7 was detected. A fluorescent
dye injected into the soma of Anti-L7 revealed that the neuron sent axonal branches to the branchial nerve. These results
may show that Anti-L7 antagonizes L7 at the periphery inside the gill, rather than in the abdominal ganglion. EJPs induced
by L7 were unaffected by Anti-L7. Activation of Anti-L7 alone did not induce any change in tone or membrane potential of the
gill musculature. The suppressive effect of Anti-L7 lasts many seconds after the cessation of a train of Anti-L7 impulses.
The results may suggest that the suppression is mediated through an inhibitory neuromodulatory mechanism without inhibition
of L7 itself.
Accepted: 1 April 1999 相似文献
57.
We tested the hypothesis that a pressure difference can cause blebbing associated with uncoupling of the plasma membrane from the cortical actin, a phenomenon found earlier in locomoting blebbing Walker carcinosarcoma cells. Untreated, initially spherical Walker carcinosarcoma cells were exposed to suction pressure by partial aspiration into micropipettes. The suction pressure required to induce blebbing was in the range of 0.9-3 cm H2O, i.e., somewhat lower than the increase in intracellular pressure measured before formation of protrusions in Amoeba proteus (Yanai et al., Cell Motil. Cytoskeleton 33, 22-29, 1996). The response was temperature-dependent, blebbing occurring more frequently at 37 degrees C than at room temperature. Blebbing was associated with formation of cytoplasmic actin layers, restriction rings and/or of gaps in the plasma membrane-associated cortical actin. The results support the view that blebbing associated with uncoupling of cortical actin and plasma membrane as observed in locomoting cells can be caused by a pressure gradient. 相似文献
58.
豚鼠主动脉前庭自发性慢反应电位去极离子流的初步分析 总被引:15,自引:3,他引:12
为研究主动脉前庭自发慢反应电位的去极离充性质,利用豚鼠的离体以及心脏,常规玻璃微电极细胞内记录方法和离子通道组断剂,观测最大舒张电位(MDP)、0相除极幅度(APA)、0相最大除极速度(Vmax)、4个自动除极速度(VDD)、复极50%(APD50)和90%(APD90)的时间以及自发放电频率(RPF)。结果发现:⑴0.5μmol/L尼索地平(Nis)可使该慢电位的APA、Vmax、VDD明显减小 相似文献
59.
目的:三维超声心动图评估不同年龄段和性别主动脉瓣二叶畸形(bicuspid aortic valve malformation,BAV)的应用价值。方法:超声心动图检测我院2012年6月至2014年9月70例BAV患者,根据年龄段分为4组,≤20岁年龄组7例,21-40岁年龄组14例,41-60岁年龄组30例,≥60岁年龄组19例;根据性别分为2组,男性42例,女性28例。测量不同病例分组的主动脉窦部及升部内径、室间隔厚度、左心功能及左房横径,比较主动脉瓣狭窄、关闭不全、钙化及脱垂四个合并症发生率。结果:在心脏结构指标方面,年龄段分组室间隔厚度≥60岁年龄组12.37±1.64 mm高于≤20岁年龄组10.43±2.22 mm和21-40岁年龄组11.00±1.92 mm;左房横径41-60岁年龄组38.73±7.95 mm和≥60岁年龄组40.05±9.71 mm高于≤20岁年龄组29.86±1.86 mm。性别分组左心功能女性64.18±6.04%高于男性58.71±11.28%。在合并症发生率方面年龄段分组主动脉瓣狭窄41-60岁年龄组80%、≥60岁年龄组84%高于21-40岁年龄组50%,性别分组狭窄男性81%大于女性54%,关闭不全女性79%大于男性50%。结论:三维超声心动图诊断BAV可获得更加全面、具体、直观的诊断信息,BAV的超声表现与患者年龄段和性别密切相关。 相似文献
60.
目的:探讨硬膜外复合全麻对老年高血压患者腹部手术后并发生的影响。方法:收集2013 年6 月-2015 年6 月在两家医院
接受腹部手术的老年高血压患者100 例,根据麻醉方法不同分为研究组和对照组。研究组患者给予硬膜外复合麻醉,对照组给予
全凭静脉麻醉。观察并比较两组患者的麻醉时间、药物用量以及术后并发症的发生率。结果:两组麻醉时间比较,差异无统计学意
义(P>0.05);研究组麻醉药物用量低于对照组,差异具有统计学意义(P<0.05);两组患者手术时间比较,差异无统计学意义(P>0.
05);研究组患者术后苏醒时间、拔管时间以及住院时间均短于对照组,差异具有统计学意义(P<0.05);研究组术后并发症的发生
率低于对照组,差异具有统计学意义(P<0.05)。结论:硬膜外复合全麻用于老年高血压患者腹部手术能够减少麻醉药物用量,降低
术后并发症的发生率,安全性较高。 相似文献