盐肤木种子繁殖试验

本文对盐肤木种子的理化处理、种子质量、贮藏方法、发芽温度、播种时期、深度及其他的繁殖方法的试验结果进行了报道,为提高盐肤木的种子发芽率和成苗率及培育强壮的幼苗提供了可行的措施．     

多功能种子带

多功能种子带是由农作物秸秆制成纸浆,根据生产不同作物的需要加入不同配方的氮、磷、钾肥,锌、硼等微量元素及某些农药、生长调节剂、除草剂,将上述纸浆制成一定厚度的种子带,将种子按一定规格播入带中,在带的表面喷涂薄薄的一层有机薄膜涂料,然后干燥卷成筒入库。上述生产流程完全是在多功能种子带生产机中完成,它可生产多种种子的多功能种子     

多个地区植物区系丰富的综合评判

本文介绍了模糊数学中综合评判的原理及二种方法,通过贵州,云南,四川,广西,湖南,湖北,广东,安徽,海南等九省区种子植物区系科,属,种数目的分析,解决了多地区植物区系丰富性综合比较及其评价这一问题。结果与实际情况基本一致,尤其方法Ⅱ比方法Ⅰ的效果更佳。     

金沙江干热河谷种子植物区系特征的初探

研究涉及金沙江云南境内的干热河谷和干暖河谷的种子植物区系。建立名录共920种,有野生植物748种。这748种,分属于111科,427属。427属的分布区类型的格局为：l－44,2—127,3—10,4—39,5—13,6—37,7—29,8—47,9—12,10—25,11—3,12—7,13—l,14—20,15—13．在748种中,三江（金沙江、元江、怒江）干热河谷共有的标志植物73种,金沙江的标志植物156种,它们是研究区系特征的基础。经分析,本区植物区系有以下特征：(1)属、种均以热带成分为主,热带属占66.58%,种的热带成分更多,均交错少数温带成分;(2)三江干热河谷共同标志植物中,多数是优势种或常见种,例如Heteropogon contortus, Bombax ceiba,Phyllanthus emblica,Euphorbia royleana,Tribulus terrestris等,地区特有成分则少,如Terminalia franchetii,Andropogon yunnanensis;(3)滇北、川西南河谷特有成分则较多,如Cycas panzhihuaensis,Nouelia insignis,Aristolochia delavayi等;(4)西南特有成分也较多,且在河谷常见,如Delavaya yunnanensis,Croton yunnanensis,Munronia delavayi等;(5)有一些非特有的特殊种类,如Heteropogon melanocarpus,Dinebra retroflexa等.鉴于特征分析,本区植物区系有以下几个更深层的特征:(1)热带起源与近代交错现象;(2)有古地中海区系的后裔;(3)与近代非洲与南亚区系有联系;(4)与华北区系有联系;(5)特有现象明显而成因复杂,等。     

基于非靶代谢组学的多花黄精沙藏种子萌发过程差异代谢物分析

低温沙藏处理可以有效解除多花黄精种子休眠,为此本实验利用非靶向气相色谱-质谱法（GC-MS）技术鉴定并分析了沙藏后的多花黄精种子不同萌发时期的代谢产物及其变化规律。共鉴定出初级代谢产物116种,对多花黄精种子萌发0 d vs.7 d,7 d vs.14 d,14 d vs.21 d,21 d vs.28 d和28 d vs.35 d等比较组进行成对比较筛选,分别筛选鉴定出各比较组中发生显著变化的差异代谢物（VIP>1.0,P<0.05）17,27,9,7和1种。多花黄精种子萌发初期的0 d、7 d和14 d,种子内的二糖（海藻糖,麦芽糖,蔗糖等）以及TCA循环中苹果酸,琥珀酸,柠檬酸等含量显著增加,而在14 d后整体呈现下降趋势。γ-氨基丁酸等氨基酸含量在萌发0 d时最高。HCA分析表明,大部分的代谢物含量在沙藏的多花黄精种子萌发7 d时上升,14 d后下降,表明在14 d前后初级代谢产物被快速消耗。KEGG分析表明,种子萌发过程中TCA循环,精氨酸与脯氨酸代谢,淀粉和蔗糖代谢等多条代谢通路表现活跃。在萌发初期,氨基酸、糖类和有机酸类物质含量三者间显著相关。本研究结果为进一步开展黄精种子人工繁育提供了理论依据。     

罗汉果生物学特性初步研究

<正> 罗汉果(Momordica grosvenori Swingle)为我国特有经济植物,畅销国内外市场。当前罗汉果产量和品质有不同程度的下降,因此研究其生物学特性,为制定罗汉果的栽培技术,提高产量与品质提供科学依据。     

古生代种子的新分类系统

本文概述了上世纪中叶以来有关古生代种子分类问题的沿革,并在总结近年来国内外有关整体 化石裸子植物和离体种子研究新成果的基础上,指出当前在古生代种子分类方面的问题。 为使古生代种子的分类同当代古植物学研究协调,把对离体种子的研究同整体化石裸子植物结合 起来,作者建议以Seward的分类系统为基础,设立五个科：瓶籽科Lagenostomaceae肥籽科 Pachytestaceae、靓籽科Callospermariaceae隐羊齿籽科Cryptospermaceae和心籽科Cardiocarpaceae, 并把三棱籽目Trigonocarpales改名为肥籽目Pachytestales。上述五个科既可看作三个古生代种子目： 瓶籽目Lagenostomales、肥籽目和心籽目Cardiocarpales下设的一级分类单位,也可分别当作古生代整 体化石裸子植物：皱羊齿目Lygonopteridales、髓木目Medullosales、靓羊齿目Callistophytales、大羽羊 齿目Gigantopteridales和科达目Cordaitales的雌性生殖器官。由于这个分类系统同整体化石植物的分类一致,因此,有助于对离体种子的系统学研究。     

ABA-Mediated Inhibition of Germination Is Related to the Inhibition of Genes Encoding Cell-Wall Biosynthetic and Architecture： Modifying Enzymes and Structural Proteins in Medicago truncatula Embryo Axis

Radicle emergence and reserves mobilization are two distinct programmes that are thought to control germination. Both programs are influenced by abscissic acid （ABA） but how this hormone controls seed germination is still poorly known. Phenotypic and microscopic observations of the embryo axis of Medicago truncatula during germination in mitotic inhibition condition triggered by 10 μM oryzalin showed that cell division was not required to allow radicle emergence. A suppressive subtractive hybridization showed that more than 10% of up-regulated genes in the embryo axis encoded proteins related to cell-wall biosynthesis. The expression of α-expansins, pectin-esterase, xylogucan-endotransglycosidase, cellulose synthase, and extensins was monitored in the embryo axis of seeds germinated on water, constant and transitory ABA. These genes were overexpressed before completion of germination in the control and strongly inhibited by ABA. The expression was re-established in the ABA transitory-treatment after the seeds were transferred back on water and proceeded to germination. This proves these genes as contributors to the completion of germination and strengthen the idea that cell-wall loosening and remodeling in relation to cell expansion in the embryo axis is a determinant feature in germination. Our results also showed that ABA controls germination through the control of radicle emergence, namely by inhibiting cell-wall loosening and expansion.     

Preparation and preliminary application of monoclonal antibodies against Trichokirin-S1, a small ribosome-inactivating peptide from the seeds of Trichosanthes kirilowii

Trichokirin-S1,a small ribosome-inactivating peptide recently purified from the seeds ofTrichosanthes kirilowii,has potential clinical applications because of its small molecular mass.Two stablestrains of hybridomas (1F11 and 2A5) that can secrete highly specific monoclonal antibodies (mAbs) againstTrichokirin-S1 have been developed using the hybridoma technique.The isotypes of these two mAbs,1F11and 2A5,were determined to be IgG_(2a) and IgG_1,respectively.The affinity constants,which were measuredby non-competitive ELISA,were found to be 2.3×10~8 M~(-1) and 2.8×10~8 M~(-1),respectively.An immunoaffinitymethod using 2A5-coupled Sepharose 4B was successfully developed to purify Trichokirin-S1.These twoantibodies have also been used to detect Trichokirin-S1 in Western blot.