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本文报道了青甘直隶环毛蚓Pheretimatschiliensiskokoana的生殖器官多态现象,因卵巢发育正常,属于孤雌生殖种类,并发现一新的中间型(I6型),即前列腺与受精囊之间的中间型。 相似文献
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单链构象的多态现象(SSCP)是根据PCR技术广泛用于检测不同DNA序列的一门技术,由遗传学家开发的致突变检测,最近已采用于病毒基因组准种的分析,如丙型肝炎病毒(HCV)。由于该技术严格的标准化及其检测的局限性使之很难推广,HCV高变异区准种内的变异体被克隆到Puc 119,引起质粒定量,这种变种可用作SSCP最佳模板,所研究的变量有许多检测到的变异体,少数变异体检测的敏感性,电泳温度、单链DNA产生的方法、PCR循环次数的影响以及与校读能力有关的DNA多聚酶的应用。结果表明,它是能在准种内至少测出5种变异体的最佳方法,并能检出低至2%水平的准种内的变异体,电泳在室温18小时其重复性最好,使用Taq多聚酶单个引物作20次循环产生的单链DNA给准种构成一个精确的映象,Pfu多聚酶的应用减低了检测较小的带型的敏感性。SSCP法提供了评价HCV准种一个精确的工具。 相似文献
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在中学高中生物学教学中,因学生的“植物学”知识欠缺,理解雌球花与枝条同源等概念非常困难。笔者在指导学生研究性学习的备课过程中,发现了罕见的云杉雌球花发育逆转和大孢子叶的多态现象,遂采集了标本,用数码相机拍了照片,并作了进一步的探究,深感是直观教学的良好资料,愿与同行分享。1我校学生对校园云杉的认识云杉(Picea asperata)属裸子植物(Gymnospermae)、球果纲(Coniferopsida)、松科(Pinaceae)、云杉属(Picea),是我校校园绿化的优势树种,所以也是历届学生植物学研究性学习的必选“材料”。根据以往学生的调查记录:我校园内云杉4月中下旬开花,雌球花呈穗状,紫红色,多生于树冠中上部主、侧枝端,较雄球花迟1周 相似文献
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Yabing Wang Di Zhang Yun Liu Yifeng Yang Teng Zhao Jie Xu Sheng Li Zuofeng Zhang Guoyin Feng Lin He He Xu 《遗传学报》2009,36(7):417-423
Single-nucleotide polymorphisms (SNPs) of ADIPOQ, ADIPOR1, and ADIPOR2 have been associated with type 2 diabetes mellitus (T2DM), but there are many conflicting results especially in Chinese populations. To investigate the contribution of the adiponectin genes and their receptors to T2DM, a case-control study was performed and 11 SNPs ofADIPOQ, ADIPOR1, and ADIPOR2 were genotyped in 985 T2DM and 1,050 control subjects, rs 16861194 (-11426 A〉G) in the putative promoter of ADIPOQ was associated with T2DM (P = 0.007; OR = 1.29, 95% CI 1.08-1.55). None of the other 10 SNPs were associated with T2DM in this study, although rs2241766 and rs1501299 were reported to be associated with T2DM in previous Chinese studies. There was also no significant difference found from the ADIPOQ haplotype analysis, which contains rs 16861194. In addition, we also assessed potential gene-gene interactions in three genes and no interactions were found. In conclusion, our results supported the ADIPOQ gene as a possible risk factor for type 2 diabetes in Han Chinese population. 相似文献
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