全文获取类型
收费全文 | 10479篇 |
免费 | 142篇 |
国内免费 | 64篇 |
出版年
2023年 | 218篇 |
2022年 | 484篇 |
2021年 | 578篇 |
2020年 | 572篇 |
2019年 | 467篇 |
2018年 | 295篇 |
2017年 | 337篇 |
2016年 | 333篇 |
2015年 | 652篇 |
2014年 | 1025篇 |
2013年 | 909篇 |
2012年 | 550篇 |
2011年 | 498篇 |
2010年 | 581篇 |
2009年 | 539篇 |
2008年 | 244篇 |
2007年 | 260篇 |
2006年 | 221篇 |
2005年 | 152篇 |
2004年 | 102篇 |
2003年 | 86篇 |
2002年 | 100篇 |
2001年 | 126篇 |
2000年 | 68篇 |
1999年 | 155篇 |
1998年 | 68篇 |
1997年 | 93篇 |
1996年 | 117篇 |
1995年 | 83篇 |
1994年 | 72篇 |
1993年 | 85篇 |
1992年 | 67篇 |
1991年 | 80篇 |
1990年 | 61篇 |
1989年 | 62篇 |
1988年 | 56篇 |
1987年 | 18篇 |
1986年 | 24篇 |
1985年 | 26篇 |
1984年 | 24篇 |
1983年 | 12篇 |
1982年 | 19篇 |
1981年 | 21篇 |
1980年 | 16篇 |
1979年 | 21篇 |
1978年 | 12篇 |
1977年 | 12篇 |
1976年 | 14篇 |
1975年 | 14篇 |
1973年 | 11篇 |
排序方式: 共有10000条查询结果,搜索用时 31 毫秒
991.
P. F. Hendrix G. H. Baker M. A. Callaham Jr G. A. Damoff C. Fragoso G. González S. W. James S. L. Lachnicht T. Winsome X. Zou 《Biological invasions》2006,8(6):1287-1300
The most conspicuous biological invasions in terrestrial ecosystems have been by exotic plants, insects and vertebrates. Invasions by exotic earthworms, although not as well studied, may be increasing with global commerce in agriculture, waste management and bioremediation. A number of cases has documented where invasive earthworms have caused significant changes in soil profiles, nutrient and organic matter dynamics, other soil organisms or plant communities. Most of these cases are in areas that have been disturbed (e.g., agricultural systems) or were previously devoid of earthworms (e.g., north of Pleistocene glacial margins). It is not clear that such effects are common in ecosystems inhabited by native earthworms, especially where soils are undisturbed. We explore the idea that indigenous earthworm fauna and/or characteristics of their native habitats may resist invasion by exotic earthworms and thereby reduce the impact of exotic species on soil processes. We review data and case studies from temperate and tropical regions to test this idea. Specifically, we address the following questions: Is disturbance a prerequisite to invasion by exotic earthworms? What are the mechanisms by which exotic earthworms may succeed or fail to invade habitats occupied by native earthworms? Potential mechanisms could include (1) intensity of propagule pressure (how frequently and at what densities have exotic species been introduced and has there been adequate time for proliferation?); (2) degree of habitat matching (once introduced, are exotic species faced with unsuitable habitat conditions, unavailable resources, or unsuited feeding strategies?); and (3) degree of biotic resistance (after introduction into an otherwise suitable habitat, are exotic species exposed to biological barriers such as predation or parasitism, “unfamiliar” microflora, or competition by resident native species?). Once established, do exotic species co-exist with native species, or are the natives eventually excluded? Do exotic species impact soil processes differently in the presence or absence of native species? We conclude that (1) exotic earthworms do invade ecosystems inhabited by indigenous earthworms, even in the absence of obvious disturbance; (2) competitive exclusion of native earthworms by exotic earthworms is not easily demonstrated and, in fact, co-existence of native and exotic species appears to be common, even if transient; and (3) resistance to exotic earthworm invasions, if it occurs, may be more a function of physical and chemical characteristics of a habitat than of biological interactions between native and exotic earthworms. 相似文献
992.
Alfonso Medel-Narvaez José Luis Leon de la Luz Francisco Freaner-Martinez Francisco Molina-Freaner 《Plant Ecology》2006,187(1):1-14
Understanding the mechanisms that determine the distribution and abundance of plants is a major problem in ecology. However, very few studies have explored the factors controlling the abundance of columnar cacti throughout their range of distribution. In this paper, we describe the density and size structure of 26 populations of Pachycereus pringlei throughout its distribution range in the Sonoran Desert. Major differences in abundance were detected between island and mainland and peninsular areas, with islands sustaining significantly larger densities than mainland and peninsular populations. Within peninsular populations, the abundance was negatively associated with latitude and positively associated with annual and seasonal rainfall. In contrast, the abundance in mainland populations showed neither latitudinal trend nor an association with rainfall. In peninsular populations, mean height and basal diameter of branched plants showed a negative association with population density whereas mainland populations showed no significant association. None of the populations exhibited a population structure that fitted the log-normal distribution expected for young, growing populations with constant recruitment. Insular, peninsular and␣mainland populations showed a population structure with an uneven size distribution typical of populations experiencing regeneration pulses. 相似文献
993.
S. Syed Alwee C.G. Van der Linden J. Van der Schoot S. de Folter G.C. Angenent S-C. Cheah M.J.M. Smulders 《Plant Cell, Tissue and Organ Culture》2006,85(3):331-344
In vitro propagation of oil palm (Elaeis guineensis Jacq.) frequently induces a somaclonal variant called ‘mantled’ abnormality, in which the stamens of both male and female flowers are transformed into carpels. This leads to a reduced yield or complete loss of the harvest of palm oil. The high frequency of the abnormality in independent lines and the high reversal rate suggest that it is due to an epigenetic change. The type of morphological changes suggest that it involves homeotic MADS box genes that regulate the identity of the flower whorls. We have isolated a number of MADS box genes from oil palm inflorescences by a MADS box-directed mRNA display approach. The isolated partial cDNAs included genes that were likely to function at the initial stages of flowering as well as genes that may function in determination of the inflorescence and the identity of the flower whorls. For four genes that were homologous to genes known to affect the reproductive parts of the flower, full length cDNAs were isolated. These were a B-type MADS box gene which may function in the determination of stamen formation, a C-type gene expected to be involved in stamen and carpel formation, and two putative SEP genes which act in concert with the A-, B- and C-type MADS box gene in determining flower whorl formation. The B-type gene EgMADS16 was functionally characterized as a PISTILLATA orthologue; it was able to complement an Arabidopsis thaliana pi mutant. Whether EgMADS16, or any of the other EgMADS genes, are functionally involved in the mantled condition remains to be established. 相似文献
994.
Jen-Ping Chung Tsu-Liang Chang Albert Yu-Ming Chi Chou Tou Shii 《Plant Cell, Tissue and Organ Culture》2006,87(3):305-314
Embryogenic cell suspensions of Musa AAA and AAB genomic groups were cultured in a maintenance culture medium for 17 generations (lasting for 238 days). The cell growth phases and medium pH changes were also observed correspondingly. Three major growth phases of AAA genomic group have been focused, namely cell releasing, proliferation and globularization phases. During almost all the subculture generations the cell stocks of AAB ‘Raja’ were continuously characterized by proliferating cell aggregates while the globularization phase occurred only for short duration. The medium acidity levels of the cell stocks of AAA ‘Pei-Chiao’ and ‘Robusta’ were commonly scattered in a wider range of pH 3.3–5.3, while the AAB ‘Raja’ were deviated in a narrow range of pH 4.0–4.6. After subculture, culture medium showed biphasic pH changes, which were drastic pH falls followed by an auto-regulated steady-state level. The steady-state pH values in each of the three growth phases (i.e. cell releasing, proliferation and globularization phases) were of 3.3–4.0, 4.0–4.8 and 4.8–5.3 respectively. Morphological bipolarity and the efficiency in the formation of somatic embryos have been thoroughly discussed. Reported research indicates that the condition of pH below 4.6 may prevent the development of embryogenic cells towards polar growth. 相似文献
995.
Jun Zheng Jinfeng Zhao Jinpeng Zhang Junjie Fu Mingyue Gou Zhigang Dong Wei Hou Quansheng Huang Guoying Wang 《Plant science》2006,170(6):1125-1132
cDNA macroarray has become a useful tool to analyze expression profiles and compare the similarities and differences of various expression patterns. We have prepared a cDNA macroarray containing 190 maize expressed sequence tags (ESTs) specifically induced by water stress to analyze the expression profiles of maize seedlings under abscisic acid (ABA) treatment, high-salinity and cold stress conditions. The results indicated that 48 ESTs in leaves and 111 ESTs in roots were significantly up-regulated by ABA treatment, 36 ESTs in leaves and 41 ESTs in roots by high-salinity stress, 14 ESTs in leaves and 18 ESTs in roots by cold induction, whereas 22 ESTs were induced under all 3 stresses. Results from the hierarchical cluster analysis suggest that the leaves and roots of maize seedlings had different expression profiles after these stresses. The overlap analysis of different stress-induced ESTs indicated that there is more crosstalk between water stress and ABA and high-salinity stress than between water stress and cold stress. It will be helpful to study the precise function of the corresponding overlapping-induced genes for understanding the relationship and crosstalk between different stress signal pathways. 相似文献
996.
Effect of red mold rice on antifatigue and exercise-related changes in lipid peroxidation in endurance exercise 总被引:3,自引:0,他引:3
This study evaluated the effect of red mold rice supplementation on antifatigue and exercise-related changes in lipid peroxidation
of male adult Wistar rats through swimming exercise. Thirty 16-week-old rats were studied by dividing them into three groups
(ten for each group). Other than the control group (CD), the other two groups were divided into a high-dose (HD) treatment
group (5 g red mold rice/kg body weight for the HD group), and a low-dose (LD) group (1 g red mold rice/kg body weight for
the LD group). Swimming endurance tests were conducted after 28 days of red mold rice supplementation, and the result showed
that the treatment group showed a higher exercise time (CD, 78.0±6.4; LD, 104.2±9.6; and HD, 129.4±10.9 min; p<0.05) and a higher blood glucose concentration (CD, 76.67±8.08; LD, 111.34±8.50; and HD, 117.67±11.06 mg/dl; p<0.05) than the CD. Moreover, the blood lactate (CD, 45.00±0.90; LD, 31.41±1.80; and HD, 28.89±1.62 mg/dl; p<0.05), blood urea nitrogen (CD, 21.87±0.75; LD, 20.33±0.83; and HD, 20.53±1.09 mg/dl; p<0.05), and hemoglobin (CD, 14.20±0.21; LD, 13.70±0.55; and HD, 13.28±0.35 g/dl; p<0.05) were also significantly lower than those of the CD. Besides, the result suggested that the red mold rice supplementation
may decrease the contribution of exercise-induced oxidative stress and improve the physiological condition of the rats. 相似文献
997.
In order to investigate the levels of genetic diversity of the endangered species Kirengeshoma palmata (Saxifragaceae), four extant populations were sampled and analyzed using inter-simple sequence repeats (ISSR) markers. We expected a low genetic diversity level, but our results revealed a high level of intraspecific genetic diversity, probably resulting from this species being in a refuge during the last glaciation (at population level: P = 63.25%, Ae = 1.47, HE = 0.26 and HO = 0.37; at species level: P = 79.00%, A = 1.5538, HT = 0.2586 and Hsp = 0.3104). A low level of genetic differentiation among populations was detected based on Nei's genetic diversity analysis (16.69%) and AMOVA (19.36%). Populations shared high levels of genetic identity. Insect pollination and seed dispersal by wind may have facilitated extensive gene flow and are likely responsible for this present structure of genetic variation. 相似文献
998.
Photosynthetic pathways (e.g. C3, C4) and morphological functional types (e.g. trees, shrubs, high perennial grasses, perennial forbs) were identified for the native species from the Saskatchewan mixed
prairie, using the data from references published between 1950 and 2003. Of the total 219 identified species in 145 genera
and 45 families, 208 species in 137 genera and 44 families were found with C3 photosynthesis, and most of these species are dominants (e.g. Agropyron dasystachyum Hook. and Stipa spartea var. curtiseta Hitchc.). 11 species in 10 genera and 3 families were identified with C4 photosynthesis (e.g. Atriplex argentea Nutt., Andropogon scoparius Michx., Boutelou gracilis Lag., Calamovilfa longifolia Hook.). The amount of total identified C4 species in the region is much less than that from the South Dakota mixed prairie (27 species). Gramineae is the leading family with C4 photosynthesis (8 species), Chenopodiaceae ranks the second (2 species). Relatively less forb types [50 % perennial forbs (PEF) and 12 % annual forbs (ANF)] and more
graminoid types (25 %) composition suggested that the rangelands in the region are relatively stable. Lacking of the knowledge
on the optimal traits for PFTs classification in the region, further studies (e.g. C3 and C4 plant identification and optimal trait selection) are needed to explore the relationships between PFTs and vegetation variations,
as well as land-use and climate changes. 相似文献
999.
Huang EJ Wu CC Huang HP Liu JY Lin CS Chang YZ Lin JA Lin JG Chen LM Lee SD Kuo WW Huang CY 《Molecular and cellular biochemistry》2006,290(1-2):1-7
Since there is evidence for estrogen and estrogen-like compounds to have beneficial effect on the pathogenesis of hepatocellular
carcinoma (HCC), this study was designed to investigative the apoptotic and anti-proliferative effects of these compounds
on the human hepatoma Hep3B cell line. The Hep3B cells were treated with 17β-estradiol (E2), diethylstilbestrol (DES), tamoxifen,
and genistein. After treatments of these compounds at the concentration of 10-6 or 10-8 M, the Hep3B cells were demonstrated to have significant DNA fragmentation, nucleus condensation, cytochrome-c leaking from
the mitochondria and caspase-3 activation by DAPI and Western blotting. The cells were also observed to have declined proliferative
potential by MTT assay, arrested cell cycle by flow-cytometry measurements. However, the cytochrome-c leaking from the mitochondria
induced by E2 and E2-like compounds was blocked totally by ICI 182,780 treatment. These finding suggest that estrogen and
the estrogen-like compounds may induce anti-proliferative and apoptotic effects in Hep3B cells, and the E2 and the E2-like
compounds mediated apoptotic effect was estrogen receptor dependent. Among the drugs tested, E2, E2 agonists (DES and genistein)
and partial antagonist (tamoxifen), all showed the stronger anti-tumor potential.
The last two authors, Wei-Wen Kuo and Chih-Yang Huang, share equal contribution. 相似文献
1000.
In an attempt to elucidate the effects of Sporothrix schenckii infection on the immune response, our laboratory has developed a murine model of disseminated sporotrichosis. Helper T cells
can be further subdivided into Th1 and Th2 phenotypes. The differentiation of two subsets of T lymphocytes is driven by IL-12
and IL-4 cytokines, respectively. Th1 cells produce IFN-γ that activate macrophages and promote cell-mediated immunity. In
addition, we found low levels of iNOS and NO production in the initial (1st and 2nd weeks) and final (9th and 10th weeks)
periods of the infection, in contrast with the period of week 4 to 7 of elevated values. The determination of IFN-γ and IL-12
are in agreement with NO/iNOS detection, showing the presence of cellular immune response throughout the infectious process.
However, the production of IL-4 shows an increase in levels after the 5th and 6th weeks suggesting a participation of Th2
response in this period as well. Regarding these results, the study demonstrated that in experimental sporotrichosis infection
the cellular immune response participated throughout the period analyzed as a nitric oxide dependent mechanism. In contrast,
the presence of Th2 response began in the 5th week, suggesting the participation of humoral immune response in advanced stages
of sporotrichosis. 相似文献