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将马立克氏病病毒(Marek's disease virus,MDV)gB基因插入真核表达载体pcDNA3中,经酶切和PCR鉴定为正向插入了gB基因的重组质粒,命名为pcDNA3-gB。体外转染COS-7细胞,经间接免疫荧光染色证实转染pcDNA3-gB后的COS-7细胞内有糖蛋白B的表达。用pcDNA3-gB对从肉用雏鸡腿部肌肉注射,进行一免。间隔2周后,用pcDNA3-gB再加强免疫一次。之后2周攻毒,观察免疫保护效果。结果表明,MDV gB基因DNA免疫可以诱导鸡体产生免疫保护,免疫保护指数为72.2%。 相似文献
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将马立克氏病病毒(Marek's disease virus,MDV)gB基因插入真核表达载体pcDNA3中,经酶切和PCR鉴定为正向插入了gB基因的重组质粒,命名为pcDNA3-gB.体外转染COS-7细胞,经间接免疫荧光染色证实转染pcDNA3-gB后的COS-7细胞内有糖蛋白B的表达.用pcDNA3-gB对AA肉用雏鸡腿部肌肉注射,进行一免.间隔2周后,用pcDNA3-gB再加强免疫一次.之后2周攻毒,观察免疫保护效果.结果表明,MDV gB基因DNA免疫可以诱导鸡体产生免疫保护,免疫保护指数为72.2%. 相似文献
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番鸭细小病毒强、弱毒株VP2基因的序列测定比较 总被引:2,自引:0,他引:2
According to the complete nucleotide sequence of Muscovy duck parvovirus registered in the gene bank, two modified primers (LHMP7/LHMP8) were designed and, for each of them, a restriction endonuclease recognition site, SacⅡ or KpnⅠ, was included respectively. The DNA encoding VP2 structural protein of the wild strain MDPV Q and the attenuated strain MDPV 26 which had been derived by continued passage of virulent wild type (MDPV Q) in Muscovy duck embryo were amplified by PCR and recombined into T vector and sequenced respectively. It shows that both DNA encoding VP 2 structural protein of MDPV Q and MDPV which has been registered in the gene bank had the high homology (97.9%). MDPV 26 and MDPV Q displayed 99.7% identity, and shared 98.3% homology with that of reported MDPV. 相似文献
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