Preliminary Studies of Root-Stem Transition in Brassica napus L.

Seedlings of Brassica napus L. 2–11 days after germination were used. However, the most investigation was concentrated on the 6-day old seedlings. The primary root has a diarch protostele, the two groups of primary phloem alternate with the primary xylem. At higher level, the metaxylem is gradually differentiated in a lateral direction. Being coincident with this changes of the metaxylem, the groups of phloem cell are extended. The stele of the lower hypocotyl is root-like and has no pith. In the middle hypocotyl, there is a further lateral differentiation of the metaxylem. At the higher level, four metaxylem arms appear and the groups of phloem are extended circumferentially to form two crescent shaped sectors. In the upper hypocotyl below 0.2 cm of the cotyledonary node, a central pith has been formed which separates the differentiating primary xylem into two distinct units. At a slightly higher level, each primary phloem divides into two small groups, at this time, each xylem unit and the two adjacent groups of phloem constitute a cotyledonary trace. The foliar traces of the first two foliage leaves appear in the inter-cotyledonary plane between the vascular elements of the cotyledonary traces. At this level, the vascular tissue of the hypocotyl forms a siphonostele made up of two cotyledonary traces and the two foliage leaves, where the root-stem transition has nearly been completed, while the endarch condition is not attained in the hypocotyl. At incresing distances from the cotyledonary node upwards, in the cotyledonary petiole, the protoxylem occupies a more and more adaxial position and the metaxylem a more and more abaxial direction and, thus, the endarch condition is attained. The primary system of the root, hypocotyl, and cotyledons forms a complete circular system, the plumular vascular elements are directly connected by secondary elements formed by the cambium in the region of the hypocotyl. As for the results mentioned above, the authers have not detected that the primary xylem has a rotation of 180˚, as described by Van Tieghem.     

大鼠脑红蛋白(NGB)可溶性原核表达和单克隆抗体制备及鉴定

脑红蛋白 (NGB)是新发现的脑特异的携氧蛋白 .为了对其功能进行深入的研究 ,应用已构建大鼠NGB基因编码区原核表达载体pGEX 4T 2 NGB转化的大肠杆菌BL2 1 ,获得可溶性表达产物 .用谷胱甘肽S 转移酶 (GST)亲和层析柱纯化后作为抗原 ,免疫Balb c小鼠 .通过传统的细胞融合方法制备了抗大鼠NGB的单克隆抗体 ,并对全部 4株单抗进行了亚类和亚型、效价和特异性鉴定 .为NGB结构与功能的深入研究提供了重要工具     

编码天麻抗真菌蛋白cDNA的分子克隆

用重组ＤＮＡ 技术研究了编码天麻抗真菌蛋白（ＧＡＦＰ）的基因,从天麻（Ｇａｓｔｒｏｄｉａ ｅｌａｔａＢｌ．）块茎中提取Ｐｏｌｙ（Ａ） ｍ ＲＮＡ 后合成ｃＤＮＡ,构建成表达型ｃＤＮＡ 文库,用纯化的蛋白质探针通过免疫筛选找出对应的ｃＤＮＡ 克隆。在进一步证明所选用的ｃＤＮＡ 克隆含有重组的λ－ｐｈａｇｅ ＤＮＡ 后,提取和纯化含有插入片段重组子的ＤＮＡ,用Ｅｃｏ ＲＩ酶切分析可见插入片段。已分离出编码天麻抗真菌蛋白的基因     

抗中性粒细胞胞浆抗体、抗核抗体联合检测对类风湿关节炎的诊断价值

目的：探讨抗中性粒细胞胞浆抗体（ANCA）与抗核抗体（ANA）联合检测对类风湿关节炎的临床意义。方法：采用IIF法对82例RA患者（RA组）、74例非RA自身免疫疾病患者（非RA组）和52例健康体检者（正常对照组）的血清ANCA和ANA谱进行了检测分析,并用ELISA法进行抗丝氨酸蛋白酶3（PR3）、抗髓过氧化物酶（MPO）、ANA谱的定量检测。结果：RA组82例患者中,64例ANCA阳性,阳性率为78．08％,其中核周型（PANCA）37例,阳性率为45．1％,胞浆型（CANCA）27例,阳性率为32．9％;非RA组74例患者中有7例ANCA阳性率分别为9．4％;正常对照组50例中没有一例ANCA阳性。利用Elisa法对患者血清进行检测,分别能够特异的检测到PR3、MPO、抗双链DNA抗体（抗ds—DNA抗体）、抗SS—A等抗体、抗ss—A抗体、抗PM—SCL抗体的存在。结论：联合ANCA、ANA检测有助于提高类风湿关节炎的诊断。     

穹窿切断后海马神经元GR表达变化的研究

目的实施大鼠穹窿切断术研究海马神经元核受体GR(glucocorticoidreceptor糖皮质激素受体)表达的变化。方法建立大鼠穹窿切断模型,于穹窿切断术后0、4、7、10d取材;同时取材假手术组(非穹窿切断术)作为对照,应用免疫组化、WesternBlotting方法分别进行各组海马神经元GR表达变化的观察及定量检测。结果穹窿切断7d后,免疫组化和WesternBlotting结果显示海马神经元GR表达下调,10d下调更为显著。结论穹窿切断后,海马GR表达下调,提示可能减弱海马对HPA轴的抑制。     

融合蛋白GGH粉针剂制备工艺研究

目的：建立高效液相色谱法测定融合蛋白GGH原料和注射用粉针剂含量的方法,筛选制备粉针剂的辅料和pH,进行6个月的长期稳定性试验。方法：高效液相色谱采用Waters DELTA PAK C18色谱柱,流动相为乙腈和水,梯度洗脱20min,检测波长280nm。根据粉针剂的外形、复溶性、稳定性和活性保留率筛选填充剂及pH。结果：GGH在0.2~1.6mg/ml范围内线性关系良好,可用此方法进行制剂的含量检测;选用4%的甘露醇作为填充剂并调节pH为5.5,可以制备性质较为稳定的GGH冻干粉针剂。结论：可采用含量和纯度检测结合外观性状观察法用于粉针剂的制备工艺筛选,为申报新药提供参考。     

新疆啤酒花新品种阜北—1的成分分析比较

分析了新疆阜北农场培育的啤酒花新品种阜北－１的主要化学成分,与新疆现有的２个有规模的香型花余乐比特、札一进行比较。新品种酒花中苦味树脂的含量为１８．９％,α－酸为５．６３％,β－酸为２．２６％,合律草酮占总α－酸的３０．７３％,新品种的酒花油含量为０．９０％,其中法呢稀相对含量高达１１．０３％。从这些表征啤酒花品种特性的数据得知,新酒花属于香型花,与现有的２个香型花一样,品质较佳,应该大力推广。     

登革病毒2型E蛋白基因真核表达和DNA免疫的研究

将编码登革病毒2型(DV2)氨基末端80%的E蛋白的DNA片段克隆到真核表达载体pCXN2 AG强启动子下游,构建成DV2E重组真核表达质粒pCXN-E.间接免疫荧光显示其可在COS-7细胞中表达.ELISA法检测pCXN2-E DNA免疫BALB/c鼠血清中的E抗体变化和维持规律,结果显示三次免疫后2周已有抗体产生,15周时仍维持较高的水平;血清空斑减数中和实验显示其中和滴度高于1640;流式细胞计数仪(FACS)检测DNA免疫鼠CD4+、CD8+T淋巴细胞变化情况,与注射空载体pCXN2的阴性鼠相比,CD4+淋巴细胞水平略有上升,CD8+细胞水平有较大升高(p＜0.01);动物保护性实验结果显示,当用致死剂量登革病毒攻击免疫鼠时,其保护率为60%.以上结果表明pCXN2-E在实验动物内表达出的DV2E蛋白可以诱导免疫动物的体液免疫和细胞免疫应答,尤其是MHC-Ⅰ限制性杀伤性CD8+T淋巴细胞水平的提高对清除病毒是十分有利的.因此,DV2 E DNA免疫为登革病毒DNA疫苗的发展进行了有益的探索.     

56例卵巢上皮性交界性肿瘤的临床和病理分析

目的：探讨卵巢交界性上皮肿瘤（OBT）的临床及病理特征,以供临床和病理借鉴.方法：收集病理快速冰冻切片和石蜡切片确诊的交界性上皮性卵巢肿瘤患者56例,术前均行超声检查及CA125测定.结果：56例OBT中以粘液性肿瘤最多,为33例,占58.9%,浆液性肿瘤23例占41.1%.其中以Ⅰ期多见,手术包括保守性手术治疗交界性肿瘤,预后良好.冰冻切片诊断卵巢交界性上皮肿瘤的总体准确率为64.2%.结论：诊断OBT需根据临床症状、肿瘤标记物及影像学综合判断,术中冰冻切片检查有一定帮助,但最后诊断还是应主要依据病理切片.为提高快速冰冻切片诊断的准确率,应做连续切片.