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111.
112.
SYNOPSIS. the pattern of RNA synthesis of intracellular Trypanosoma cruzi amastigotes, immediately following infection of Lesch-Nyhan human fibroblasts, was studied by autoradiography. Amastigote RNA synthesis, determined by [3 H]guanine incorporation, was not detected until 2 h after infection. At 8 h postinfection more than 90% of intracellular amastigotes were labeled. It was verified that extracellular trypomastigotes also synthesized RNA. Therefore it was concluded that, if RNA is required for trypomastigote-to-amastigote transformation, this nucleic acid is already present in the trypomastigotes before infection of the vertebrate cell. It is probable that the RNA synthesized by amastigotes during the prereplicative lag period (the period between initial infection and the onset of DNA synthesis) is required for intracellular growth and reproduction. 相似文献
113.
The formulation of disability (or “functional impairment”) is currently not operationalized in either the ICD or the DSM. In the DSM system, making a diagnosis depends on a conjoint assessment of symptoms and functioning, whereas the ICD keeps the disability construct separate from the diagnosis of mental disorders. We need an internationally agreed conceptualization between ICD and DSM in terms of better operationalization of disease and disability components. No functioning or disability should appear as part of the threshold of the diagnosis in either system. 相似文献
114.
115.
LAURE MICHELET THOMAS ROACH BEAT B. FISCHER MARIETTE BEDHOMME STÉPHANE D. LEMAIRE ANJA KRIEGER‐LISZKAY 《Plant, cell & environment》2013,36(6):1204-1213
In photosynthetic organisms, excess light is a stress that induces production of reactive oxygen species inside the chloroplasts. As a response, the capacity of antioxidative defence mechanisms increases. However, when cells of Chlamydomonas reinhardtii were shifted from dark to high light, a reversible partial inactivation of catalase activity was observed, which correlated with a transient increase in the level of H2O2 in the 10 μm range. This concentration range seems to be necessary to activate H2O2‐dependent signalling pathways stimulating the expression of H2O2 responsive genes, such as the heat shock protein HSP22C. Catalase knock‐down mutants had lost the transient accumulation of H2O2, suggesting that a decrease in catalase activity was the key element for establishing a transient H2O2 burst. Catalase was inactivated by a one‐electron event consistent with the reduction of a single cysteine. We propose that under high light intensity, the redox state of the photosynthetic electron transport chain is sensed and transmitted to the cytosol to regulate the catalase activity. This allows a transient accumulation of H2O2, inducing a signalling event that is transmitted to the nucleus to modulate the expression of chloroplast‐directed protection enzymes. 相似文献
116.
Rute ST Martins Laurence AM Deloffre Constantinos C Mylonas Deborah M Power Adelino VM Canário 《Reproductive biology and endocrinology : RB&E》2007,5(1):19
Background
DAX1 (NR0B1), a member of the nuclear receptors super family, has been shown to be involved in the genetic sex determination and in gonadal differentiation in several vertebrate species. In the aquaculture fish European sea bass, Dicentrarchus labrax, and in the generality of fish species, the mechanisms of sex determination and differentiation have not been elucidated. The present study aimed at characterizing the European DAX1 gene and its developmental expression at the mRNA level. 相似文献117.
RONALD C KESSLER MATTHIAS ANGERMEYER JAMES C ANTHONY RON DE GRAAF KOEN DEMYTTENAERE ISABELLE GASQUET GIOVANNI DE GIROLAMO SEMYON GLUZMAN OYE GUREJE JOSEP MARIA HARO NORITO KAWAKAMI AIMEE KARAM DAPHNA LEVINSON MARIA ELENA MEDINA MORA MARK A OAKLEY BROWNE JOSé POSADA-VILLA DAN J STEIN CHEUK HIM ADLEY TSANG SERGIO AGUILAR-GAXIOLA JORDI ALONSO SING LEE STEVEN HEERINGA BETH-ELLEN PENNELL PATRICIA BERGLUND MICHAEL J GRUBER MARIA PETUKHOVA SOMNATH CHATTERJI T. BEDIRHAN üSTüN 《World psychiatry》2007,6(3):168-176
Data are presented on the lifetime prevalence, projected lifetime risk,
and age-of-onset distributions of mental disorders in the World Health Organization
(WHO)''s World Mental Health (WMH) Surveys. Face-to-face community surveys
were conducted in seventeen countries in Africa, Asia, the Americas, Europe,
and the Middle East. The combined numbers of respondents were 85,052. Lifetime
prevalence, projected lifetime risk, and age of onset of DSM-IV disorders
were assessed with the WHO Composite International Diagnostic Interview (CIDI),
a fully-structured lay administered diagnostic interview. Survival analysis
was used to estimate lifetime risk. Median and inter-quartile range (IQR)
of age of onset is very early for some anxiety disorders (7-14, IQR: 8-11)
and impulse control disorders (7-15, IQR: 11-12). The age-of-onset distribution
is later for mood disorders (29-43, IQR: 35-40), other anxiety disorders (24-50,
IQR: 31-41), and substance use disorders (18-29, IQR: 21-26). Median and IQR
lifetime prevalence estimates are: anxiety disorders 4.8-31.0% (IQR: 9.9-16.7%),
mood disorders 3.3-21.4% (IQR: 9.8-15.8%), impulse control disorders 0.3-25.0%
(IQR: 3.1-5.7%), substance use disorders 1.3-15.0% (IQR: 4.8-9.6%), and any
disorder 12.0-47.4% (IQR: 18.1-36.1%). Projected lifetime risk is proportionally
between 17% and 69% higher than estimated lifetime prevalence (IQR: 28-44%),
with the highest ratios in countries exposed to sectarian violence (Israel,
Nigeria, and South Africa), and a general tendency for projected risk to be
highest in recent cohorts in all countries. These results document clearly
that mental disorders are commonly occurring. As many mental disorders begin
in childhood or adolescents, interventions aimed at early detection and treatment
might help reduce the persistence or severity of primary disorders and prevent
the subsequent onset of secondary disorders. 相似文献
118.
Primers for 15 microsatellite loci were developed for Mountain Plover, a species whose distribution and abundance have been reduced drastically in the past 30 years. In a screen of 126 individuals collected from four breeding locales across the species’ range, levels of polymorphism ranged from two to 13 alleles per locus. No two loci were found to be linked, although one locus revealed significant departures from Hardy–Weinberg equilibrium. These microsatellite loci can be used in population genetic studies, ultimately aiding in management efforts for Mountain Plover. Additionally, these markers can potentially be used in studies investigating the mating system of Mountain Plover. 相似文献
119.
JB Farinha DL Dos Santos G Bresciani LF Bard F de Mello ST Stefanello AA Courtes FAA Soares 《Biology of sport / Institute of Sport》2015,32(2):109-114
The aim of this study was to investigate the impact of moderate aerobic training on functional, anthropometric, biochemical, and health-related quality of life (HRQOL) parameters on women with metabolic syndrome (MS). Fifteen untrained women with MS performed moderate aerobic training for 15 weeks, without modifications of dietary behaviours. Functional, anthropometric, biochemical, control diet record and HRQOL parameters were assessed before and after the training. Despite body weight maintenance, the patients presented decreases in waist circumference (P = 0.001), number of MS components (P = 0.014), total cholesterol (P = 0.049), HDL cholesterol (P = 0.004), LDL cholesterol (P = 0.027), myeloperoxidase activity (P = 0.002) and thiobarbituric acid-reactive substances levels (P = 0.006). There were no differences in total energy, carbohydrate, protein and lipid intake pre- and post-training. Furthermore, improvements in the HRQOL subscales of physical functioning (P = 0.03), role-physical (P = 0.039), bodily pain (P = 0.048), general health (P = 0.046) and social functioning scoring (P = 0.011) were reported. Despite the absence of weight loss, aerobic training induced beneficial effects on functional, anthropometric, biochemical and HRQOL parameters in women with MS. 相似文献
120.
DENNIS M. SCHMATZ MARK ST. J. CRANE P. KEITH MURRAY 《The Journal of eukaryotic microbiology》1984,31(1):181-183
An anion exchange column of DE-52 has been used to purify Eimeria sporozoites from a post-excystation mixture of oocysts, oocyst shells, sporocysts, sporocyst shells, and sporozoites. The mean recovery from several experiments was 94% and virtually all non-sporozoite material was removed. Infectivity studies in vitro with sporozoites showed that they were viable after purification and were at least as infectious as the unpurified sporozoites; furthermore, oocysts in the crude preparation could be recovered from the DE-52 cellulose by resuspending them in a 20% (w/v) sodium chloride solution. 相似文献