抗阿特拉津转基因大豆植株后代的遗传分析

本试验用阿特拉津溶液涂抹、荧光诱导动力学检测、分子杂交等方法对抗阿特拉津转基因大豆植株的后代进行了鉴定,在第二代及第三代中检测到了抗性基因的存在,表明从龙葵中得到的此抗阿特拉津 psbA 基因不仅能导人大豆叶绿体基因组中获得表达,而且可以遗传到后代。     

香薷中的化学成分

从香[Eltholtzia ciliata(Thund.)Hyland]中分出14个化合物,用波谱和化学等方法确定为6-甲基三十三烷(Ⅰ),13-环己基二十六烷(Ⅱ),β-谷甾醇(Ⅲ),棕榈酸(Ⅳ_a),亚油酸(Ⅳ_b),亚麻酸(Ⅳ_c),熊果酸(Ⅴ),5-羟基-6、7-二甲氧基黄酮(Ⅵ),5-羟基-7、8-二甲氧基黄酮(Ⅶ),5、7-二羟基-4′-甲氧基黄酮(Ⅷ),5-羟基-7、4′-二甲氧基双氢黄酮醇(Ⅸ),β-谷甾醇-3-β-D-葡萄糖甙(Ⅹ),5-羟基-6-甲基-7-O-α-D-半乳吡喃糖双氢黄酮甙(Ⅺ),刺槐素-7-O-β-D-葡萄糖甙(Ⅻ)。其中化合物Ⅺ为新化合物,除(Ⅳ_b)和(Ⅳ_c)外,其余为首次从该属植物中得到。     

The macrosatellites of the Toulouse goose: the major tandemly repetitive DNA in the toulouse goose genome

This paper examines the principal classes of repetitive DNA of the Toulouse goose (Anser anser) genome. There are four major classes and they are tandem repeats of less than 200 base pairs (bp). The longest repeat (class A) is 190 bp long and starts with a HinfI site. Class B is 43 bp long, commencing with a FokI site. Classes A and B show no extensive homology to DNA sequences held on a current data base (Genbank) but were confirmed to exist as major repeats in another strain of goose, the Emden goose (Anser anser) genome. Classes C and D are 5-bp repeats of 5' GAGAG 3' and 5' GGGAA 3', respectively. The macrosatellites C and D were compared with a current data base (Genbank) and were found to exist in a variety of other organisms as satellites.     

Flavonoids as superoxide scavengers and antioxidants

The superoxide anions scavenging activity and antioxidation of seven flavonoids--quercetin, rutin, morin, acacetin, hispidulin, hesperidin, and naringin--were studied. The superoxide anions were generated in a phenazin methosulphate-NADH system and were assayed by reduction of nitroblue tetrazolium. The scavenging activity ranked: rutin was the strongest, and quercetin and naringin the second, while morin and hispidulin were very weak. The concentration values yielding 50% inhibition of lipid peroxidation in mouse liver homogenate were in order of 10(-6) M for quercetin, rutin, and morin; and of 10(-5) M for acacetin and hispidulin, while naringin and hesperidin had no antioxidative action. In comparison with the antioxidative and scavenging activities of flavonoids, there are no correlations.     

金线鲃属鱼类一新种(鲤形目:鲤科)

作者于1986年6月在贵州盘县一溶洞的水体中采到金线鲃属鱼类一新种,命名为角金线鲃,新种Sinocyclocheilus angularis sp. nov.。本新种形状怪异,头背缘末端具一朝前的角状突,其内有顶骨突;眼正常;侧线鳞明显;鳔后室发达等特征而易与金线鲃属其他种相区别。     

Close association of a DNA replication origin and an ARS element on chromosome III of the yeast, Saccharomyces cerevisiae.

Two dimensional gel electrophoretic techniques were used to locate all functional DNA replication origins in a 22.5 kb stretch of yeast chromosome III. Only one origin was detected, and that origin is located within several hundred bp of an ARS element.     

On the generalization of the Weinbaum-Jiji bioheat equation to microvessels of unequal size; the relation between the near field and local average tissue temperatures

The extensive series of experiments reported in Lemons et al. [1] show that measureable local tissue temperature fluctuations are observed primarily in the vicinity of the 100-500 micron countercurrent vessels of the microcirculation and thus strongly support the basic hypothesis in the new bioheat equation of Weinbaum and Jiji [2] that these countercurrent microvessels are the principal determinants of local blood-tissue heat transfer. However, the detailed temperature profiles in the vicinity of these vessels indicate that large asymmetries in the local temperature field can result from the significant differences in size between the countercurrent artery and vein. Using the superposition techniques of Baish et al. [9], the paper first presents a solution to the classic problem of an unequal countercurrent heat exchanger with heat loss to the far field. This solution is then used to generalize the Weinbaum-Jiji bioheat equation and the conductivity tensor that appears in this equation to vessels of unequal size. An asymptotic analysis has also been developed to elucidate the relationship between the near field temperature of the artery-vein pair and the local average tissue temperature. This analysis is used to rigorously prove the closure approximation relating the local arterial-venous temperature difference and the mean tissue temperature gradient which had been derived in [2] using a more heuristic approach.     

紫茎泽兰的光合作用特征及其生态学意义

本文研究了紫茎泽兰(Eupatorium adenophorum Spreng)光合作用强度的变化规律及其与环境主要生态因子的关系,比较了它与某些农作物叶片净光合速率的差异,得到如下结果: 1.紫茎泽兰是一种阳性偏阴的C_3类草本植物,其光合作用的光饱和点约为40000 lx,光补偿点约为700 lx,且具有80 ppm左右的CO_2补偿点。 2.紫茎泽兰的最大净光合速率能达到23毫克CO_2/平方分米·时左右,叶片净光合速率的日变化规律呈双峰曲线型(主峰在10时左右,次峰在16时左右)。在一年中有较长的时间,它的光合速率保持着较高的水平。 3.生长在一般菜园土上的紫茎泽兰,当土壤含水量降至17％左右时,叶片光合速率接近0:而且,受过干旱处理的紫茎泽兰植株,在恢复供水后的第三天,其光合速率只达到原来的53％。 根据以上结果,结合受紫茎泽兰危害地区干湿季分明的特点,提出干季是防除紫茎泽兰的最佳季节。     

云南哀牢山南部鸟类的区系分析

哀牢山南延部分在云南省南部至东南部红河哈尼族彝族自治州(简称红河州)内,呈西北东南走向。南部为热带气候,北部为亚热带气候区。地势起伏虽不若滇西北三大峡谷之剧烈,但河口县与金平县内西隆山峰之高差却约达3,000米。这一南延部分区域内,植被类型复杂,花果繁茂,昆虫种类丰富。如此优越的自然条件,也导致了鸟类种类繁多昌盛的结果。     

白腹锦鸡鸣声的声谱分析

1986年1月—8月,12月及翌年1月,我们在云南省昆明市西部山区进行白腹锦鸡野外生态观察期间,录制了白腹锦鸡的鸣声。本文就啼叫声、呼唤声、惊叫声、恐惧叫声、威胁叫声及召唤雏鸟声等6种意义比较明确的鸣声进行了声谱分析,探讨各种鸣声与其相应的行为关系。