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41.
Razia Khan Zeenat Khan Bhatt Nikhil Devecha Jyoti Madamwar Datta 《Bioremediation Journal》2014,18(2):147-157
Soil samples isolated from dye-contaminated sites were exploited for isolation of dye decolorizing microorganisms. A novel bacterial mixture, RkNb1, was selected based on its efficiency, showing maximum and faster decolorization of textile dyes. Seven bacterial strains were isolated and identified from the bacterial mixture as Ochrobactrum intermedium (HM480365), Ochrobactrum intermedium strain M16-10-4 (HM030758), Enterococcus faecalis (HM480367), Arthrobacter crystallopoietes (HM480368), Kocuria flavus (HM480369), Bacillus beijingensis (HM480370), and Citrobacter freundii (HM480371) by 16S rRNA gene sequence analysis. This bacterial mixture showed 98.17% decolorization of Reactive Violet 5 (400 mg L?1) within 8 h. The culture exhibited good decolorization ability at pH 8 and at a temperature of 37°C. Malt extract and peptone was found to enhance the decolorization rate of Reactive Violet 5. Plackett-Burman experimental design was used for elucidation of medium components affecting Reactive Violet 5 decolorization. Dye degradation products obtained during the course of decolorization were analyzed by high-performance thin-layer chromatography (HPTLC), Fourier transform infrared (FTIR), and nuclear magnetic resonance (NMR). The potential of this bacterial mixture to decolorize Reactive Violet 5 dye from manufacturing industry effluent is to be carried out using appropriate bioreactors. 相似文献
42.
We report the isolation and chemical characterization of collagen cross-linking compounds, 3-hydroxypyridinium and dihydroxylysinonorleucine, from human urine. 相似文献
43.
Nocturnal behaviors that vary as a function of light intensity, either from the setting sun or the moon, are typically labeled
as circadian or circalunar. Both of these terms refer to endogenous time-dependent behaviors. In contrast, the nightly reproductive
and feeding behaviors of Vargula annecohenae, a bioluminescent ostracod (Arthropoda: Crustacea) fluctuate in response to light intensity, an exogenous factor that is
not strictly time-dependent. We measured adult and juvenile activity of V. annecohenae throughout lunar cycles in January/February and June 2003. Overnight and nightly measurements of foraging and reproductive
behavior of adult V. annecohenae indicated that activity was greatest when a critical “dark threshold” was reached and that the dark threshold for adult V. annecohenae is met when less than a third of the moon is visible or at the intensity of light 2–3 min before the start of nautical twilight
when no moon is illuminated. Juvenile V. annecohenae were also nocturnally active but demonstrated little or no response to lunar illumination, remaining active even during brightly
moonlit periods. In addition to light level, water velocity also influenced the behaviors of V. annecohenae, with fewer juveniles and adults actively foraging on nights when water velocity was high (>25 cm/s). Our data demonstrate
that the strongest environmental factor influencing adult feeding and reproductive behaviors of V. annecohenae is the availability of time when illumination is below the critical dark threshold. This dependence on darkness for successful
growth and reproduction allows us to classify darkness as a resource, in the same way that the term has been applied to time,
space and temperature. 相似文献
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Daria Wojtal Dwi?U. Kemaladewi Zeenat Malam Sarah Abdullah Tatianna?W.Y. Wong Elzbieta Hyatt Zahra Baghestani Sergio Pereira James Stavropoulos Vincent Mouly Kamel Mamchaoui Francesco Muntoni Thomas Voit Hernan?D. Gonorazky James?J. Dowling Michael?D. Wilson Roberto Mendoza-Londono Evgueni?A. Ivakine Ronald?D. Cohn 《American journal of human genetics》2016,98(1):90-101
Clustered regularly interspaced short palindromic repeat (CRISPR) has arisen as a frontrunner for efficient genome engineering. However, the potentially broad therapeutic implications are largely unexplored. Here, to investigate the therapeutic potential of CRISPR/Cas9 in a diverse set of genetic disorders, we establish a pipeline that uses readily obtainable cells from affected individuals. We show that an adapted version of CRISPR/Cas9 increases the amount of utrophin, a known disease modifier in Duchenne muscular dystrophy (DMD). Furthermore, we demonstrate preferential elimination of the dominant-negative FGFR3 c.1138G>A allele in fibroblasts of an individual affected by achondroplasia. Using a previously undescribed approach involving single guide RNA, we successfully removed large genome rearrangement in primary cells of an individual with an X chromosome duplication including MECP2. Moreover, removal of a duplication of DMD exons 18–30 in myotubes of an individual affected by DMD produced full-length dystrophin. Our findings establish the far-reaching therapeutic utility of CRISPR/Cas9, which can be tailored to target numerous inherited disorders. 相似文献
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