排序方式: 共有134条查询结果,搜索用时 609 毫秒
41.
Multiple myeloma (MM) is an incurable plasma cell (PC) malignancy characterized by the accumulation of monoclonal PCs in the
bone marrow. For deeper understanding of the molecular mechanisms involved in the development of this disease, the influence
of microenvironment, or the prediction of response of tumor PCs to anti-MM treatment, it is possible to use modern technologies
for genomic and proteomic analyses. Due to progress in instrumentation, one of the main tools of proteomic analysis is mass
spectrometry in combination with chosen separation techniques. This review will provide a short survey of the most commonly
used proteomic techniques and show examples of their applications in MM proteome studies. 相似文献
42.
Chin Chin Lee Wai Hoe Chin Archna P. Gupta Zbynek Bozdech Graham V. Brown Michael F. Duffy 《Molecular microbiology》2013,87(6):1167-1182
Histone variants are important components of eukaryotic chromatin and can alter chromatin structure to confer specialized functions. H2B variant histones are rare in nature but have evolved independently in the phyla Apicomplexa and Trypanasomatida. Here, we investigate the apicomplexan‐specific Plasmodium falciparum histone variant Pf H2B.Z and show that within nucleosomes Pf H2B.Z dimerizes with the H2A variant Pf H2A.Z and that Pf H2B.Z and Pf H2A.Z occupancy correlates in the subset of genes examined. These double‐variant nucleosomes also carry common markers of euchromatin like H3K4me3 and histone acetylation. Pf H2B.Z levels are elevated in intergenic regions across the genome, except in the var multigene family, where Pf H2A.Z/Pf H2B.Z double‐variant nucleosomes are only enriched in the promoter of the single active var copy and this enrichment is developmentally regulated. Importantly, this pattern seems to be specific for var genes and does not apply to other heterochromatic gene families involved in red blood cell invasion which are also subject to clonal expression. Thus, Pf H2A.Z/Pf H2B.Z double‐variant nucleosomes appear to have a highly specific function in the regulation of P. falciparum virulence. 相似文献
43.
Structure of Bombyx mori chemosensory protein 1 in solution 总被引:2,自引:0,他引:2
Jansen S Chmelík J Zídek L Padrta P Novák P Zdráhal Z Picimbon JF Löfstedt C Sklenár V 《Archives of insect biochemistry and physiology》2007,66(3):135-145
Chemosensory Proteins (CSPs) represent a family of conserved proteins found in insects that may be involved in chemosensory functions. BmorCSP1 is expressed mainly in antennae and legs of the silkworm moth Bombyx mori and was cloned from antennal cDNA. Here we report the determination of the structure of Bombyx mori CSP1 (BmorCSP1) by NMR. The overall fold of BmorCSP1 is globular and comprises six alpha-helices. These helices span residues 10-14, 17-27, 35-49, 57-72, 75-85, and 92-100. The internal hydrophobic sides of the helices are formed mostly by leucine and isoleucine residues and, therefore, well suited to constitute a binding site for hydrophobic ligands. 相似文献
44.
45.
46.
47.
Bures Z 《Biological cybernetics》2012,106(2):111-122
In the nervous system, the representation of signals is based predominantly on the rate and timing of neuronal discharges.
In most everyday tasks, the brain has to carry out a variety of mathematical operations on the discharge patterns. Recent
findings show that even single neurons are capable of performing basic arithmetic on the sequences of spikes. However, the
interaction of the two spike trains, and thus the resulting arithmetic operation may be influenced by the stochastic properties
of the interacting spike trains. If we represent the individual discharges as events of a random point process, then an arithmetical
operation is given by the interaction of two point processes. Employing a probabilistic model based on detection of coincidence
of random events and complementary computer simulations, we show that the point process statistics control the arithmetical
operation being performed and, particularly, that it is possible to switch from subtraction to division solely by changing
the distribution of the inter-event intervals of the processes. Consequences of the model for evaluation of binaural information
in the auditory brainstem are demonstrated. The results accentuate the importance of the stochastic properties of neuronal
discharge patterns for information processing in the brain; further studies related to neuronal arithmetic should therefore
consider the statistics of the interacting spike trains. 相似文献
48.
49.
Tvrzová L Prokop Z Navrátilová J Müllerová R Neca J 《Journal of microbiological methods》2006,65(3):551-556
A microtiter plate-based assay was developed for the automatic monitoring of degradation profile of the yellow-coloured nitrophenolic compounds. The method enables to reduce the intervals between measurements of substrate concentration to minutes and to overcome the problem of discontinuity of sampling typical for conventional methods. The concentrations of nitrophenolic compounds were calculated from the absorbance values determined automatically by BIOSCREEN C. Verification of the method was based on the comparison of results with the conventional HPLC method results. The values of the rate and saturation constants were comparable for both the microtiter plate-based assay and the conventional HPLC method. The automatic method described here seems to be efficient for the screening degradation studies, which requires the treatment of quantity of samples. 相似文献
50.
Irene Julca Daniela Mutwil-Anderwald Vaishnervi Manoj Zahra Khan Soak Kuan Lai Lay K.Yang Ing T.Beh Jerzy Dziekan Yoon P.Lim Shen K.Lim Yee W.Low Yuen I.Lam Seth Tjia Yuguang Mu Qiao W.Tan Przemyslaw Nuc Le M.Choo Gillian Khew Loo Shining Antony Kam James P.Tam Zbynek Bozdech Maximilian Schmidt Bjoern Usadel Yoganathan Kanagasundaram Saleh Alseekh Alisdair Fernie Hoi Y.Li Marek Mutwil 《植物学报(英文版)》2023,65(6):1442-1466
Plants accumulate a vast array of secondary metabolites,which constitute a natural resource for pharmaceuticals.Oldenlandia corymbosa belongs to the Rubiaceae family,and has been used in traditional medicine to treat different diseases,including cancer.However,the active metabolites of the plant,their biosynthetic pathway and mode of action in cancer are unknown.To fill these gaps,we exposed this plant to eight different stress conditions and combined different omics data capturing gene expressi... 相似文献