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41.
Si Zhang Zhongnan Yin Fei-Fei Dai Hao Wang Meng-Jiao Zhou Ming-Hui Yang Shu-Feng Zhang Zhi-Feng Fu Ying-Wu Mei Ming-Xi Zang Lixiang Xue 《Journal of cellular physiology》2019,234(8):13252-13262
Although cardiac hypertrophy is widely recognized as a risk factor that leads to cardiac dysfunction and, ultimately, heart failure, the complex mechanisms underlying cardiac hypertrophy remain incompletely characterized. The nuclear receptor peroxisome proliferator-activated receptor δ (PPARδ) is involved in the regulation of cardiac lipid metabolism. Here, we describe a novel PPARδ-dependent molecular cascade involving microRNA-29a (miR-29a) and atrial natriuretic factor (ANF), which is reactivated in cardiac hypertrophy. In addition, we identify a novel role of miR-29a, in which it has a cardioprotective function in isoproterenol hydrochloride-induced cardiac hypertrophy by targeting PPARδ and downregulating ANF. Finally, we provide evidence that miR-29a reduces the isoproterenol hydrochloride-induced cardiac hypertrophy response, thereby underlining the potential clinical relevance of miR-29a in which it may serve as a potent therapeutic target for heart hypertrophy treatment. 相似文献
42.
Liu Jingna Zang Huadong Xu Heshui Zhang Kai Jiang Ying Hu Yuegao Zeng Zhaohai 《Plant and Soil》2019,438(1-2):85-99
Plant and Soil - Moso bamboo (Phyllostachys edulis) invasions into adjacent forests are becoming increasingly common. Moso bamboo invasions affect litter quality, soil nutrients, and microbial... 相似文献
43.
Weiguo Wang Zhengguang Wang Shijie Chen Xiaofang Zang Jinglei Miao 《Journal of cellular biochemistry》2019,120(2):1763-1772
So far, microRNA has attracted plenty of interest due to its role in tumorigenesis. Reportedly, miR-181b may be involved in the tumorigenesis of osteosarcoma (OS). In the current study, we attempted to investigate the detailed function and mechanism of miR-181b in OS carcinogenesis. Herein, miR-181a, miR-181b, miR-181c, and miR-181d expressions in OS tissues were higher than that in nontumor tissue samples as examined real-time polymerase chain reaction. Via direct targeting, miR-181b negatively regulated the expression of phosphatase and tensin homolog (PTEN), a well-known tumor suppressor. Furthermore, a small interfering RNA strategy was used to find that interleukin (IL)-1B and nuclear factor-κB (NF-κB) regulate miR-181b and PTEN expression. Consequently, the repression of PTEN by miR-181b promotes OS cell proliferation. In summary, our data support a critical role for NF-κB-dependent upregulation of miR-181b, which further inhibited PTEN expression and promoted the cell proliferation of OS cell lines. The above findings represent a new pathway for the repression of PTEN and the promotion of cell proliferation upon IL-1β induction. 相似文献
44.
Immune evasion genes from filarial nematodes 总被引:12,自引:0,他引:12
Maizels RM Gomez-Escobar N Gregory WF Murray J Zang X 《International journal for parasitology》2001,31(9):889-898
45.
F H Falcone P Loke X Zang A S MacDonald R M Maizels J E Allen 《Journal of immunology (Baltimore, Md. : 1950)》2001,167(9):5348-5354
46.
47.
Manjeshwar S Lerner MR Zang XP Branam DE Pento JT Lane MM Lightfoot SA Brackett DJ Jupe ER 《Journal of molecular histology》2004,35(6):639-646
The prohibitin 3 untranslated region (3UTR) belongs to a novel class of non-coding regulatory RNAs. It arrests cell cycle progression by blocking G1-S transition in breast and other cancers. Our previous studies comparing MCF7 derived clones constitutively expressing a common allelic form of prohibitin RNA (UTR/C) to various controls demonstrated that it functions as a tumor suppressor. Here, we further characterized the morphology and motility of these transgenic breast cancer cells when grown in cell culture and on nude mice. In contrast to empty vector (EV) cells, UTR/C cells were observed to grow in an organized manner with more cell-cell contact and differentiate into structures with a duct-like appearance. Computer assisted cytometry to evaluate differences in nuclear morphology was performed on UTR/C and EV tissues from nude mice. Receiver operator curve areas generated using a logistic regression model were 0.8, indicating the ability to quantitatively distinguish UTR/C from EV tissues. Keratinocyte growth factor-induced motility experiments showed that migration of UTR/C cells was significantly reduced (80–90%) compared to EV cells. Together, these data indicate that this novel 3UTR influences not only the tumorigenic phenotype but also may play a role in differentiation and migration of breast cancer cells. 相似文献
48.
The goal of this study was the development of a method for quantitative expression proteomics on the limited sample amounts obtained through laser capture microdissection (LCM) of tissues, e.g., approximately 10 000 cells, which typically contain roughly 1-4 microg protein. The 16O/18O labeling method was selected as an approach to measure differential expression. A sample preparation protocol including lysis, digestion and 16O/18O labeling was first developed for LCM cell samples. The selected protocol was examined using two LCM caps of 10 000 cells from invasive ductal carcinoma of the breast and shown to be repeatable. A further test of LC-IT-MS/MS in combination with the 16O/18O post-digestion labeling method for studying low level samples was conducted first on a single protein (BSA) and then on a 5-standard protein mixture digest of different protein amounts, each with a total content approximately 1 microg. Next, protein expression was compared between 10 000 cells, each of microdissected normal ductal epithelium and metastatic ductal carcinoma, using the developed method. The proteins from the microdissected cells were extracted, precipitated, digested with trypsin and then 16O/18O labeled. The normal and metastatic cell samples were analyzed using reversed phase LC-ESI-MS/MS on the ion trap mass spectrometer. A total of 76 proteins were identified. Some, such as mitochondrial isocitrate dehydrogenase, actin and 14-3-3 protein xi/delta were found to be significantly up-regulated in the breast tumor cells. 相似文献
49.
50.
Improved paclitaxel accumulation in cell suspension cultures of Taxus chinensis by brassinolide 总被引:3,自引:0,他引:3
When brassinolide was added at 17 ng l–1 to Taxus chinensis cell suspension cultures, the paclitaxel content was increased by over 100% to 580 g g–1 dry weight. Brassinolide may therefore be a novel regulator of paclitaxel biosynthesis. 相似文献