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991.
992.
Kiminori Toyooka Yumi Goto Satoru Asatsuma Masato Koizumi Toshiaki Mitsui Ken Matsuoka 《The Plant cell》2009,21(4):1212-1229
Secretory proteins and extracellular glycans are transported to the extracellular space during cell growth. These materials are carried in secretory vesicles generated at the trans-Golgi network (TGN). Analysis of the mammalian post-Golgi secretory pathway demonstrated the movement of separated secretory vesicles in the cell. Using secretory carrier membrane protein 2 (SCAMP2) as a marker for secretory vesicles and tobacco (Nicotiana tabacum) BY-2 cell as a model cell, we characterized the transport machinery in plant cells. A combination of analyses, including electron microscopy of quick-frozen cells and four-dimensional analysis of cells expressing fluorescent-tagged SCAMP2, enabled the identification of a clustered structure of secretory vesicles generated from TGN that moves in the cell and eventually fuses with plasma membrane. This structure was termed the secretory vesicle cluster (SVC). The SVC was also found in Arabidopsis thaliana and rice (Oryza sativa) cells and moved to the cell plate in dividing tobacco cells. Thus, the SVC is a motile structure involved in mass transport from the Golgi to the plasma membrane and cell plate in plant cells. 相似文献
993.
Nobue Ueki Kazumasa Miyake Masafumi Kusunoki Tomotaka Shindo Tetsuro Kawagoe Seiji Futagami Taku Tsukui Hirofumi Inagaki Choitsu Sakamoto 《Helicobacter》2009,14(2):91-99
Background: The establishment of an optimal second-line regimen for Helicobacter pylori infection is required. Although quadruple therapy should overcome resistance to either clarithromycin or metronidazole, the effects of a quadruple regimen in second-line therapy are unknown. This study aims to evaluate the efficacy of triple therapy composed of proton pump inhibitor/amoxicillin plus metronidazole with the combined additive effects of clarithromycin as a second-line quadruple therapy against H. pylori infection.
Materials and Methods: Participants were 104 patients in whom first-line therapy containing proton pump inhibitor-amoxicillin-clarithromycin failed. Before starting second-line therapy, patients underwent endoscopy to obtain H. pylori strain for antibiotic susceptibility tests. Patients were randomized to receive rabeprazole (10 mg), amoxicillin (750 mg), and metronidazole (250 mg), either with clarithromycin (200 mg; RAMC group) or without (RAM group); all treatments were administered twice daily for 7 days. H. pylori eradication was confirmed by13 C-urea breath tests performed 2 to 3 months post-therapy.
Results: As shown by intention-to-treat/per-protocol analyses, the cure rates for H. pylori infection were 88.5%/93.9% and 82.7%/84.3% for the RAMC and RAM groups. Although the study probably had an insufficient power to show a significant difference between the cure rates of the two regimens, the eradication rates showed a clear trend in favor of the RAMC group. There were no severe side-effects in any group.
Conclusions: In Japan, the RAMC regimen is thought to be a promising alternative strategy for second-line eradication of H. pylori infection. 相似文献
Materials and Methods: Participants were 104 patients in whom first-line therapy containing proton pump inhibitor-amoxicillin-clarithromycin failed. Before starting second-line therapy, patients underwent endoscopy to obtain H. pylori strain for antibiotic susceptibility tests. Patients were randomized to receive rabeprazole (10 mg), amoxicillin (750 mg), and metronidazole (250 mg), either with clarithromycin (200 mg; RAMC group) or without (RAM group); all treatments were administered twice daily for 7 days. H. pylori eradication was confirmed by
Results: As shown by intention-to-treat/per-protocol analyses, the cure rates for H. pylori infection were 88.5%/93.9% and 82.7%/84.3% for the RAMC and RAM groups. Although the study probably had an insufficient power to show a significant difference between the cure rates of the two regimens, the eradication rates showed a clear trend in favor of the RAMC group. There were no severe side-effects in any group.
Conclusions: In Japan, the RAMC regimen is thought to be a promising alternative strategy for second-line eradication of H. pylori infection. 相似文献
994.
Kenji Yoshikawa Shozo Kobayashi Yumi Nakamoto Noriyasu Haginoya Satoshi Komoriya Toshiharu Yoshino Tsutomu Nagata Akiyoshi Mochizuki Kengo Watanabe Makoto Suzuki Hideyuki Kanno Toshiharu Ohta 《Bioorganic & medicinal chemistry》2009,17(24):8221-8233
A series of cis-1,2-diaminocyclohexane derivatives possessing a 6–6 fused ring for the S1 moiety were synthesized as novel factor Xa (fXa) inhibitors. The synthesis, structure–activity relationship (SAR), and physicochemical properties are reported herein, together with the discovery of compound 45c, which has potent anti-fXa activity, good physicochemical properties and pharmacokinetic (PK) profiles, including a reduced negative food effect. 相似文献
995.
Tohno S Tohno Y Hayashi M Minami T Moriwake Y Azuma C Ohnishi Y 《Biological trace element research》2003,95(2):173-184
To examine whether the calcium accumulation in aged arteries is related to the way of walking, the mineral contents were determined
in the arteries of Japanese monkeys of quadrupedal walk by inductively coupled plasma-atomic emission spectrometry. Sixteen
Japanese monkeys consisting of 7 males and 9 females ranging in age from 2 to 33 yr were studied.
The accumulation of calcium, phosphorus, and magnesium occurred progressively in most, but not all, of the arteries with aging.
It was found that independent of the upper and lower limbs, a higher accumulation of calcium, phosphorus, and magnesium occurred
in the arteries of the proximal regions with aging, compared with the arteries of the distal regions.
In a comparison between the arteries of anatomically corresponding regions of the upper and lower limbs, the accumulation
of calcium and magnesium was 20–60% higher in the external iliac and femoral arteries of the lower limb than in the axillary
and brachial arteries of the upper limb. Regarding phosphorus, the accumulation was 20–120% higher in the external iliac and
femoral arteries than in the axillary and brachial arteries. It was known that in humans, the accumulation of calcium, phosphorus,
and magnesium was three to seven times higher in the arteries of the lower limb than in the arteries of the upper limb. It
is clear that there is a very significant difference in the accumulation of calcium and magnesium in the arteries of the lower
limbs between Japanese monkeys and humans. The present study suggests that the accumulation of calcium and magnesium in the
arteries of the lower limb with aging is affected by the way of walking. 相似文献
996.
Ueki T Adachi T Kawano S Aoshima M Yamaguchi N Kanamori K Michibata H 《Biochimica et biophysica acta》2003,1626(1-3):43-50
Since the beginning of the last century, it has been known that ascidians accumulate high levels of a transition metal, vanadium, in their blood cells, although the mechanism for this curious biological function remains unknown. Recently, we identified three vanadium-binding proteins (vanabins), previously denoted as vanadium-associated proteins (VAPs) [Zool. Sci. 14 (1997) 37], from the cytoplasm fraction of vanadium-containing blood cells (vanadocytes) of the vanadium-rich ascidian Ascidia sydneiensis samea. Here, we describe the cloning, expression, and analysis of the metal-binding ability of vanabins. Recombinant proteins of two independent but related vanabins, vanabin1 and vanabin2, bound to 10 and 20 vanadium(IV) ions with dissociation constants of 2.1x10(-5) and 2.3x10(-5) M, respectively. The binding of vanadium(IV) to these vanabins was inhibited by the addition of copper(II) ions, but not by magnesium(II) or molybdate(VI) ions. Vanabins are the first proteins reported to show specific binding to vanadium ions; this should provide a clue to resolving the problem regarding the selective accumulation of vanadium in ascidians. 相似文献
997.
Krutzik SR Ochoa MT Sieling PA Uematsu S Ng YW Legaspi A Liu PT Cole ST Godowski PJ Maeda Y Sarno EN Norgard MV Brennan PJ Akira S Rea TH Modlin RL 《Nature medicine》2003,9(5):525-532
The expression and activation of Toll-like receptors (TLRs) was investigated in leprosy, a spectral disease in which clinical manifestations correlate with the type of immune response mounted toward Mycobacterium leprae. TLR2-TLR1 heterodimers mediated cell activation by killed M. leprae, indicating the presence of triacylated lipoproteins. A genome-wide scan of M. leprae detected 31 putative lipoproteins. Synthetic lipopeptides representing the 19-kD and 33-kD lipoproteins activated both monocytes and dendritic cells. Activation was enhanced by type-1 cytokines and inhibited by type-2 cytokines. In addition, interferon (IFN)-gamma and granulocyte-macrophage colony-stimulating factor (GM-CSF) enhanced TLR1 expression in monocytes and dendritic cells, respectively, whereas IL-4 downregulated TLR2 expression. TLR2 and TLR1 were more strongly expressed in lesions from the localized tuberculoid form (T-lep) as compared with the disseminated lepromatous form (L-lep) of the disease. These data provide evidence that regulated expression and activation of TLRs at the site of disease contribute to the host defense against microbial pathogens. 相似文献
998.
999.
1000.
Cloning and sequence analysis of endoglucanase genes from an industrial fungus, Aspergillus kawachii
Three endoglucanase genes (cel5A, cel5B, and cel61A) were cloned from an industrial fungus, Aspergillus kawachii. Yeasts transformed with these cDNAs showed endoglucanase activity in medium. Cel5A and Cel61A contained a type 1 cellulose-binding domain (CBD1) at the C-terminus of the enzyme. The putative catalytic regions of Cel5A and Cel5B showed homology with various endoglucanases belonging glycosyl hydrolase family 5 (GH5). Cel5B showed high homology with Cel5A in catalytic region, but it lacked CBD1 and linker. The cel5A contained four introns, whereas cel5B contained five introns. The putative catalytic region of Cel61A showed homology with enzymes belonging to GH61. The cel61A contained no introns. 相似文献