全文获取类型
收费全文 | 2799篇 |
免费 | 120篇 |
国内免费 | 2篇 |
出版年
2022年 | 8篇 |
2021年 | 19篇 |
2020年 | 8篇 |
2019年 | 11篇 |
2018年 | 28篇 |
2017年 | 16篇 |
2016年 | 40篇 |
2015年 | 57篇 |
2014年 | 57篇 |
2013年 | 257篇 |
2012年 | 153篇 |
2011年 | 138篇 |
2010年 | 79篇 |
2009年 | 90篇 |
2008年 | 138篇 |
2007年 | 141篇 |
2006年 | 174篇 |
2005年 | 158篇 |
2004年 | 162篇 |
2003年 | 176篇 |
2002年 | 162篇 |
2001年 | 52篇 |
2000年 | 48篇 |
1999年 | 41篇 |
1998年 | 46篇 |
1997年 | 40篇 |
1996年 | 22篇 |
1995年 | 32篇 |
1994年 | 28篇 |
1993年 | 33篇 |
1992年 | 40篇 |
1991年 | 37篇 |
1990年 | 35篇 |
1989年 | 33篇 |
1988年 | 21篇 |
1987年 | 23篇 |
1986年 | 28篇 |
1985年 | 25篇 |
1984年 | 25篇 |
1983年 | 25篇 |
1982年 | 26篇 |
1981年 | 29篇 |
1980年 | 26篇 |
1979年 | 19篇 |
1978年 | 16篇 |
1977年 | 18篇 |
1976年 | 9篇 |
1975年 | 10篇 |
1973年 | 9篇 |
1969年 | 9篇 |
排序方式: 共有2921条查询结果,搜索用时 62 毫秒
91.
Ken Inouye Kunio Watanabe Yoshihiro Tochino Masashi Kobayashi Yukio Shigeta 《Biopolymers》1981,20(9):1845-1858
The trypsin-catalyzed coupling of bovine (Boc)2-desoctapeptide (B23-B30)-insulin with synthetic octapeptides, H-Gly-X2-X3-X4-Thr-Pro-Lys(Boc)-Thr-OH (X2 = Phe or Ala, X3 = Phe or Ala, X4 = Tyr or Ala), followed by deprotection and purification produced the [AlaB24, ThrB30]-, [AlaB25, ThrB30]-, and [AlaB26, ThrB30]-analogs of bovine insulin in yields of 32, 35, and 32%, respectively. The biological activity of these analogs decreased in the order, normal insulin ([ThrB30]-bovine insulin) = AlaB26-insulin > AlaB25-insulin > AlaB24-insulin, as assayed for receptor binding and some other biological effects, in contrast with the corresponding Leu-analogs of human insulin, in which the activity decreased in the order, normal insulin > LeuB24-insulin > LeuB25-insulin. The affinity to insulin antibodies greatly diminished in both AlaB24-insulin and LeuB24-insulin but not in the B25-substituted analogs. The CD spectra of the Leu- and the Ala-analogs were compared with those of normal insulins to show that no apparent correlation seems to exist between the decrease in biological activity and the conformational changes observed in solution. The effects of organic solvents on the peptide-bond equilibrium and on the stability of trypsin are also discussed. 相似文献
92.
Cartilage-derived factor (CDF) : II. Somatomedin-like action on cultured chondrocytes 总被引:2,自引:0,他引:2
Yukio Kato Yoshio Nomura Mitsuko Tsuji Hiroaki Ohmae Masahiko Kinoshita Shinji Hamamoto Fujio Suzuki 《Experimental cell research》1981,132(2):339-347
Previously, we showed that fetal bovine cartilage contains a polypeptide that stimulates the incorporation of [35S]sulfate into proteoglycans synthesized by rat and rabbit costal chondrocytes in culture. In this paper, we report that the cartilage-derived factor (CDF) increases not only [35S]sulfate incorporation but also [3H]thymidine incorporation into rabbit chondrocytes in monolayer culture. The dose-response curve of CDF stimulation of DNA synthesis was similar in profile to that of CDF stimulation of proteoglycan synthesis. In addition, CDF markedly enhanced [3H]uridine incorporation into rabbit chondrocytes and significantly enhanced [3H]serine incorporation into total protein. These findings indicate that fetal bovine cartilage contains a factor that shows somatomedin-like activity in monolayer cultures of rabbit chondrocytes. 相似文献
93.
Yuji Inada Yukio Tazawa Attula Attygalle Yuji Saito 《Biochemical and biophysical research communications》1980,96(4):1586-1591
Four enzymes in urea cycle and inorganic pyrophosphatase were immobilized simultaneously into a matrix of fibrin fiber formed from fibrinogen by the concerted action of thrombin and blood coagulation Factor XIII. The immobilized multienzyme system not only had an ability to carry out urea cycle continuously at least over several hours, but also had a greatly improved efficiency over the corresponding soluble system. 相似文献
94.
The effects of intracerebroventricular administrations of three natural angiotensins, angiotensin I (ANG I 3.8 X 10-11-9.4 X10-10 mol/kg body weight), II (9.6 X 10-12-2.4 X 10-10 mol/kg body weight) and III (2.7 X 10-10 2.5 X 10-9 mol/kg body weight) on systemic blood pressure were investigated in conscious rats. Angiotensin II (ANG II), ANG I and angiotensin III (ANG III), increased blood pressure in a dose-related manner. The order of potency of angiotensins was ANG II greater than ANG I greater than ANG III. The intraventricular administration of a converting enzyme inhibitor (SQ 14225, 6.9 X10-8 mol/kg) abolished the central effect of ANG I, while an angiotensin II analogue ([Sar1-Ala8]ANG II, 1.1 X 10-8 mol/kg) administered intraventricularly inhibited the central pressor effects of these three angiotensins. These results suggest that ANG II is a main mediator of the renin-angiotensin system in the central nervous system. 相似文献
95.
In in vitro tests, amines were screened for the inhibition of polar filament extrusion by spores ofPlistophora anguillarum, a microsporidian parasite of the eel. Primary amines having C8
− C18 alkyl chains were effective, irrespective of the anions bound. Secondary amines having a branched C8 chain and tertiary amines having C14−C18 chains were also effective. Asymmetry in side chains seemed to be required for inhibitory action. Quaternary ammonium salts
having C12−C16 alkyl chains and some germicides or disinfectants such as cetyl pyridinium chloride and benzethonium chloride were also effective.
Diamines and amides were ineffective regardless of the length of their alkyl chins. In in vivo tests, eel larvae-fed for 4
days with a commercial feed supplemented with 5×108 cells of spores and 4g of laurylamine aspartate/100 g feed, and thereafter with the usual commercial feed for 26 days—were
not infected. 相似文献
96.
Minoru Okuma Yukio Yamori Kiyoe Ohta Haruto Uchino 《Prostaglandins & other lipid mediators》1979,17(1):1-7
Activities of aortae to produce prostaglandin (PG) I2-like substance in stroke-prone spontaneously hypertensive rats (SHRSP), stroke-resistant SHR (SHRSR) and normotensive control rats from the Wistar-Kyoto (WK) colony were compared. PGI2-like substance was produced by the incubation of the aortic ring in pH 9.0 borate-buffered saline and the amount produced was estimated by comparison of its anti-aggregatory activity with that produced by known amounts of the sodium salt of synthetic PGI2. Before the development of stroke, amounts of this substance generated in SHRSP and SHRSR were significantly higher than those in WK rats (p<0.01 and p<0.02, respectively). Remarkably reduced capacity to generate PGI2-like substance was observed in some SHRSP after the development of stroke. 相似文献
97.
Enzymes of purine catabolism in soybean plants 总被引:2,自引:0,他引:2
Remarkable formation and utilization of allantoin is observedin soybean (Glycine max variety A62-1). To study this, variousenzymes involved in purine catabolism (i.e., xanthine oxidase,uricase and allantoinase) were measured in different regionsof soybean plants during development. Uricase, which catalyzesthe direct formation of allantoin from uric acid, was studiedin detail. The activities of these three enzymes were highest in the rootnodules, indicating that the nodules are the major site of allantoinmetabolism. Radicles only showed appreciable activity about80 hr after the seeds were planted. Allantoinase activity wasdetected in all regions tested, showing that allantoin translocatedfrom the nodules can be metabolized in the roots, stem and leaves.In the nodules, xanthine oxidase was localized in the nuclearfraction, while uricase was mainly restricted to the mitochondrialfraction and allantoinase to the soluble fraction. Uricase was partially purified from the nodules and radicles,respectively. The pH optimum of enzyme from the nodules was9.5, whereas that of enzyme from the radicles was 7.0. The enzymefrom the nodules did not require a cofactor, while that fromthe radicles showed an absolute requirement for a cofactor,which was a low molecular substance easily separable from theapoprotein. Thus, the uricase in nodules differs in chemicalproperties from that in the host plant. The results are discussedin relation to change in the allantoin level in soybean tissues. (Received November 1, 1974; ) 相似文献
98.
This study describes the ultrastructural characteristics of the middle cerebral artery and its related neural elements in the squirrel monkey and baboon. The cytoarchitecture of the M-1 segment as well as that of the smaller extracerebral and intracerebral vessels is comparable in both animals.Smooth muscle elements are occasionally found within the intimai lining. The nerve bundles associated with vessels contain fewer myelinated fibers as the vessel diameter decreases. The cytological relationship between the neural structures and the smooth muscle cells are discussed. 相似文献
99.
We studied the interaction between synthetic atrial natriuretic peptide (ANP) and various vasoactive substances, which included isoproterenol (ISO), aminophylline (AMI), and dibutyryl cyclic AMP (dBcAMP) as vasodilators, and angiotensin II (AII) and norepinephrine (NE) as vasoconstrictors, and prazosin as an alpha-blocker in isolated perfused rat kidneys (IPK). When 10(-9) mol of ANP was administered in 75 ml of a perfusate, the renal vascular resistance (RVR) was transiently decreased for 5 min, and increased thereafter. Simultaneously, ANP increased the glomerular filtration rate (GFR), urine flow (UV), absolute Na excretion (UNaV) and absolute K excretion (UKV). All of the above mentioned effects of ANP were significantly inhibited by administering ISO, AMI or dBcAMP. On the other hand, the administration of AII and NE significantly enhanced the increases in UV and UNaV and the fractional excretion of Na induced by ANP, although AII and NE had no influence on the changes in RVR and GFR induced by ANP. Prazosin did not modify the renal effects of ANP. These results suggest that the natriuretic effect of ANP is inhibited by agents that increase cyclic AMP in vascular smooth muscle cells. It is also suggested that the natriuretic effects of ANP can be explained by an increase in GFR and changes in intrarenal hemodynamics, rather than by the direct effect of ANP on renal tubules. 相似文献
100.
From the root bark of Hovenia dulcis Thunb. and H. tommentella (Makino) Nakai (Rhamnaceae), three peptide alkaloids, frangulanine, hovenins-A and -B have been isolated. Hovenin-A has been shown to be des-N-methylfrangulanine (II). 相似文献