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Ans Timmermans Melanie C. Melendrez Youry Se Ilin Chuang Nou Samon Nichapat Uthaimongkol Chonticha Klungthong Wudtichai Manasatienkij Butsaya Thaisomboonsuk Stuart D. Tyner Sareth Rith Viseth Srey Horm Richard G. Jarman Delia Bethell Nitima Chanarat Julie Pavlin Tippa Wongstitwilairoong Piyaporn Saingam But Sam El Mark M. Fukuda Sok Touch Ly Sovann Stefan Fernandez Philippe Buchy Lon Chanthap David Saunders 《PloS one》2016,11(3)
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Genetic engineering methods based on the use of transgenes have been successfully adopted to improve crops. A novel all-native DNA gene technology consists of the creation of intragenic constructs by isolating genetic elements from a crop, rearranging them in vitro, and inserting them back into the plant. The ever-increasing genomic information and the elucidation of the molecular mechanisms that control fruit development could be exploited to confer the desired fruit phenotypes using endogenous DNA. The spatial/temporal regulation of genes can be modified by using appropriate endogenous regulatory elements, such as fruit-specific promoters. In addition, intragenic silencing can be employed to downregulate fruit-related genes. Here, we describe the available tools for intragenic manipulation of early phases of fleshy fruit initiation. 相似文献