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961.
962.
Keiko Tanaka Ken-Ichi Iyama Mitsuhiko Kitaoka Yoshifumi Ninomiya Toshitaka Oohashi Yoshikazu Sado Tomomichi Ono 《Journal of molecular histology》1997,29(7):563-570
Type IV collagen, the major component of basement membrane, consists primarily of ·1(IV) and ·2(IV) chains. Recently, other
types of collagen IV chains, i.e. ·3(IV), ·4(IV), ·5(IV) and ·6(IV) chains, have been identified by protein chemistry and
molecular cloning. We have examined the diversity of the assembly of ·(IV) chains of the basement membrane surrounding tumour
nests of basal cell carcinomas, in tissues from 11 patients, by immunohistochemical analysis using specific monoclonal antibodies
to six ·(IV) chain. The immunostaining profile of each chain differed with respect to the histological subtypes of basal cell
carcinoma. In the morphea-like subtype, which was more invasive, ·1(IV) and ·2(IV) chains were discontinuously stained, and
·5(IV) and ·6(IV) chains were entirely absent. However, in the superficial subtype, which was non-aggressive, ·1(IV), ·2(IV),
·5(IV) and ·6(IV) chains were well stained compared with the other subtypes of basal cell carcinoma. In addition, in the solid
subtype, which showed slow growth and ulceration, ·1(IV) and ·2(IV) chains were continuously stained, and ·5(IV) and ·6(IV)
chains were discontinuous or absent. The assembly of ·5(IV) and ·6(IV) chains into the basement membrane was inhibited in
the solid and morphea subtypes of BCC. This differential expression of type IV collagen chains seems to be associated with
the invasive potential of basal cell carcinoma
This revised version was published online in November 2006 with corrections to the Cover Date. 相似文献
963.
Isolation and Chromosomal Localization of a Cornea-Specific Human Keratin 12 Gene and Detection of Four Mutations in Meesmann Corneal Epithelial Dystrophy 总被引:6,自引:0,他引:6 下载免费PDF全文
Kohji Nishida Yoichi Honma Atsuyoshi Dota Satoshi Kawasaki Wakako Adachi Takahiro Nakamura Andrew J. Quantock Hisashi Hosotani Shuji Yamamoto Masaki Okada Yoshikazu Shimomura Shigeru Kinoshita 《American journal of human genetics》1997,61(6):1268-1275
Keratin 12 (K12) is an intermediate-filament protein expressed specifically in corneal epithelium. Recently, we isolated K12 cDNA from a human corneal epithelial cDNA library and determined its full sequence. Herein, we present the exon-intron boundary structure and chromosomal localization of human K12. In addition, we report four K12 mutations in Meesmann corneal epithelial dystrophy (MCD), an autosomal dominant disorder characterized by intraepithelial microcysts and corneal epithelial fragility in which mutations in keratin 3 (K3) and K12 have recently been implicated. In the human K12 gene, we identified seven introns, defining eight individual exons that cover the coding sequence. Together the exons and introns span approximately 6 kb of genomic DNA. Using FISH, we found that the K12 gene mapped to 17q12, where a type I keratin cluster exists. In this study, four new K12 mutations (Arg135Gly, Arg135Ile, Tyr429Asp, and Leu140Arg) were identified in three unrelated MCD pedigrees and in one individual with MCD. All mutations were either in the highly conserved alpha-helix-initiation motif of rod domain 1A or in the alpha-helix-termination motif of rod domain 2B. These sites are essential for keratin filament assembly, suggesting that the mutations described above may be causative for MCD. Of particular interest, one of these mutations (Tyr429Asp), detected in both affected individuals in one of our pedigrees, is the first mutation to be identified within the alpha-helix-termination motif in type I keratin. 相似文献
964.
Yoshikazu Kitagawa Yoshitaka Manabe Joichi Oyamada Atsushi Rokutanda 《American journal of physical anthropology》1995,97(2):101-111
Morphological variations of the deciduous dentition are as useful as those of the permanent dentition for determining the biological affinities of human populations. This paper provides material on morphological variations of deciduous teeth of the prehistoric Japanese population from the Late and the Latest Jomon Period (ca. 2000–ca. 300 B.C.). The expression of nonmetric traits of the deciduous teeth in the Jomon sample shows a closer affinity with modern Japanese and Native American samples than with American White, Asiatic Indian, and African samples. However, the frequency of shoveling in deciduous upper incisors in the Jomon sample is lower than those in modern Japanese and Native American samples. The Jomon sample also expresses a much higher frequency of cusp 6 in deciduous lower second molars than seen in modern Japanese, Ainu, and Native American samples. The frequency in the Jomon sample is equal to that in the Australian Aboriginal sample, which shows cusp 6 most frequently among the samples compared. A somewhat low incidence of incisor shoveling in the Jomon sample was also reported in the permanent dentition (Turner [1976] Science 193:911–913, [1979] Am. J. Phys. Anthropol. 51:619–635, [1987] Am. J. Phys. Anthropol. 73:305–321, [1990] Am. J. Phys. Anthropol. 82:295–317; T. Hanihara [1992] Am. J. Phys. Anthropol. 88:163–182, 88:183–196). However, the frequency of cusp 6 in the Jomon sample shows no significant difference from those of Northeast Asian or Native American samples in the permanent dentition (Turner [1987] Am. J. Phys. Anthropol. 73:305–321; T. Hanihara [1992] Am. J. Phys. Anthropol. 88:1–182, 88:183–196). Evidently, some nonmetric traits express an inter-group difference only in the deciduous dentition. © 1995 Wiley-Liss, Inc. 相似文献
965.
1-Benzyl-1,2,3,4-Tetrahydroisoquinoline as a Parkinsonism-Inducing Agent: A Novel Endogenous Amine in Mouse Brain and Parkinsonian CSF 总被引:7,自引:1,他引:6
Yaichiro Kotake Yoshikazu Tasaki Yukiko Makino Shigeru Ohta Masaaki Hirobe 《Journal of neurochemistry》1995,65(6):2633-2638
Abstract: 1-Benzyl-1,2,3,4-tetrahydroisoquinoline (1BnTIQ) was detected as a novel endogenous amine in mouse brain and parkinsonian CSF by using the gas chromatography-selected ion-monitoring method. The level of 1BnTIQ was very high in CSF of some parkinsonian patients compared with that of controls with other neurological diseases, the mean value being three times higher (parkinsonians: 1.17 ± 0.35 ng/ml of CSF, n = 18; vs. controls: 0.40 ± 0.10 ng/ml of CSF, n = 11; mean ± SEM, not significantly different). The pole test, a toxicological examination to evaluate behavior abnormalities related to Parkinson's disease, was used to examine the pharmacological effect of 1BnTIQ in mice. Repeated administration of 1BnTIQ induced behavior abnormalities, which pretreatment with 1-methyl-1,2,3,4-tetrahydroisoquinoline could prevent. We suggest that 1BnTIQ may be related to the idiopathic Parkinson's disease. 相似文献
966.
967.
Selective Desulfurization of Dibenzothiophene by Rhodococcus erythropolis D-1 总被引:11,自引:0,他引:11 下载免费PDF全文
Yoshikazu Izumi Takashi Ohshiro Hiroshi Ogino Yoshimitsu Hine Masayuki Shimao 《Applied microbiology》1994,60(1):223-226
A dibenzothiophene (DBT)-degrading bacterium, Rhodococcus erythropolis D-1, which utilized DBT as a sole source of sulfur, was isolated from soil. DBT was metabolized to 2-hydroxybiphenyl (2-HBP) by the strain, and 2-HBP was almost stoichiometrically accumulated as the dead-end metabolite of DBT degradation. DBT degradation by this strain was shown to proceed as DBT → DBT sulfone → 2-HBP. DBT at an initial concentration of 0.125 mM was completely degraded within 2 days of cultivation. DBT at up to 2.2 mM was rapidly degraded by resting cells within only 150 min. It was thought this strain had a higher DBT-desulfurizing ability than other microorganisms reported previously. 相似文献
968.
Yoshikazu Horie Makoto Miyaji Kazuko Nishimura Marcello F. Franco Kunie Iabuki R. Coelho 《Mycoscience》1995,36(2):159-165
Among the isolates from soil of corn and sugar cane plantations in São Paulo State, Brazil, two new species ofNeosartorya, N. botucatensis andN. paulistensis, are described and illustrated.Neosartorya botucatensis differs from the other known species of the genus in having ascospores with long spines on the convex walls.Neosartorya paulistensis is characterized by its ascospore walls with spinose and verruculose ornamentation. The former is compared with the closely related speciesN. spinosa, and the latter is closely related toN. glabra, N. pseudofischeri and N. stramenia. 相似文献
969.
Yoshikazu Horie Makoto Miyaji Kazuko Nishimura Marcello F. Franco Kunie Labuki R. Coelho 《Mycoscience》1995,36(2):199-204
Neosartorya udagawae, a new heterothallic species isolated from Brazilian soil, is described and illustrated. It is characterized by dull green colony on malt extract agar, light yellow large ascomata, broadly lenticular ascospores with two equatorial or several irregular crests and tuberculate convex surfaces, and with anAspergillus anamorph.Neosartorya aureola is also described as a new record from Brazilian soil. 相似文献
970.
Yoshikazu Ueno 《Primates; journal of primatology》1994,35(3):301-310
Operant conditioning was used to investigate how primates discriminate between odor qualities. Eight artificial food flavors,
selected from either a “Fishy” or “Aroma/Fruity” category, were used. During the presence of one of the two odors S+ or S−,
the monkey was reinforced by pushing a response key when S + was presented. The tufted capuchins discriminated most accurately
when both odors were Aroma. Discrimination was more accurate when S+ was Fruity odor and S− was Fishy odor. When both odors
were Fishy, discrimination could not be acquired in 20 sessions. All of the flavors used, except apple, were novel for the
subjects, which suggests that capuchins can innately discriminate among them. The data also suggest that Aroma odors are more
salient than Fishy odors. The results also suggested an innate aversion to Fishy odors. 相似文献