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651.
652.
Poly(ADP-ribosyl)ation of DNA polymerase beta in vitro 总被引:1,自引:0,他引:1
Y Ohashi A Itaya Y Tanaka K Yoshihara T Kamiya A Matsukage 《Biochemical and biophysical research communications》1986,140(2):666-673
DNA polymerase beta purified from bovine thymus is markedly inhibited when incubated in a reconstituted poly(ADP-ribosyl)ating reaction system. Analyses of the reaction product synthesized in this system by SDS-polyacrylamide gel electrophoresis and subsequent fluorography of the gel indicated that ADP-ribose is covalently attached to DNA polymerase beta molecule (Mr = 44,000). 相似文献
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K Yoshihara Y Tanaka T Hashida H Ura T Kamiya Y Tanigawa S S Koide 《Biochemical and biophysical research communications》1982,106(3):803-810
We have measured ΔA transient absorption spectra in the Soret region and kinetics of photodissociation of oxymyoglobin (MbO2) solutions following excitation by pulses of duration 350 fsec and 10 μJ energy at 307 nm. We observed an instantaneous bleaching of the absorbance at 414 nm and the appearance of a broad, red-shifted absorption band in the 438–470 nm region with a time constant of 250 fsec indicative of the formation of a short-lived deliganded Mb★ species which relaxes to the stable Mb with a constant of 3.5 psec. Following this early relaxation, changes in absorption kinetics indicate also a geminate recombination process of constant τ = 100 psec. These data demonstrate that the well established low quantum yield (φ = 0.03) of photodissociation in MbO2 is related both to the relaxation of an excited Mb★ state and to a fast geminate recombination process. 相似文献
654.
Summary Non-woody fibers such as from Mitsumata (Edgeworthia papyrifera Sieb et Zucc.) bast were successfully macerated with an alkalophilic Bacillus. This made it possible to prepare pulp sheets without fibrillization by beating. Physical qualities of sheets obtained by alkaline retting compared favorably with those produced by the chemical pulping method now in use.Part VII of a series on BIOCHEMICAL PULPING 相似文献
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溴化马来松香的合成、表征及其阻燃性的研究 总被引:1,自引:0,他引:1
本文以马来松香与液溴加成,合成了溴化马来松香;研究了反应温度、原料的摩尔比、反应时间等因素对反应过程及产物性能的影响.用FT-IR、1H NMR和TG对溴化马来松香进行了表征.结果表明,当反应温度为-2~6℃,反应时间5~6 h,反应物的当量配比约为1.2时,收率可达87.9%.溴化马来松香具有较好的阻燃性,同时也拓宽了松香的应用范围,提高了松香的附加值. 相似文献
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S.D. HA F.T. JONES Y.M. KWON S.C. RICKE 《Journal of Rapid Methods and Automation in Microbiology》1997,5(1):47-59
The present study was designed to compare unirradiated Salmonella typhimurium survival during storage after inoculation in either irradiated or unirradiated poultry feed. The effects of irradiation (5 kGy) on the indigenous feed microflora and on the survival of marker strain of S. typhimurium contaminated after irradiation treatment were determined during 56 days of storage of either soybean meal (SBM) or meat and bone meal (MBM) based feeds. The initial aerobic bacterial populations were reduced more than 90% in both SBM (4.96 to 4.08 ± 0.03 log10 CPU/g feed) and MBM (5.12 to 3.90 ± 0.03) by irradiation. Irradiation treatment reduced the average fungal counts during 56 days of storage in both SBM (4.24 to 2.74 ± 0.03) and MBM (4.38 to 2.15 ± 0.03) containing feeds. However, unirradiated S. typhimurium populations inoculated after irradiation of the feed were not different in either irradiated or nonirradiated SBM and MBM based feeds. Therefore, the differences in fungal versus bacterial sensitivity among the feed types and storage times suggests that gamma irradiation can alter the makeup of indigenous microbial populations in feed but this does not appear to have a discernible influence on subsequent survival of unirradiated S. typhimurium added as a dry inoculum after irradiation. 相似文献