A Service for Human Chromosome Studies in Saskatchewan

A service has been developed in Saskatchewan to make available the results of studies of human chromosomes, the material being forwarded to the laboratory by local transport facilities. During the first year of this project chromosome studies were requested for five doubtful cases of trisomy-21 (two were found to be normal) and for 20 definite cases of trisomy-21 in young patients (two had translocations but the parents of both these children had normal karyotypes). Eleven confirmed cases of Turner''s syndrome, two of Klinefelter''s syndrome, and one each of the D and E syndromes were also studied. The largest group for which studies were requested comprised 36 patients with mental retardation; only two abnormal karyotypes were encountered in this group.     

Characterization of a Tumorlike Antigen in Type 12 and Type 18 Adenovirus-Infected Cells.

Gilead, Zvee (University of Pennsylvania, Philadelphia), and Harold S. Ginsberg. Characterization of a tumor-like antigen in type 12 and type 18 adenovirus-infected cells. J. Bacteriol. 90:120-125. 1965.-An antigen that reacts with antibody from type 12 adenovirus tumor-bearing hamsters was identified in extracts of KB cells infected with type 12 or 18 adenovirus. In contrast, viral structural proteins separated by chromatography on diethylaminoethyl-cellulose did not react with the sera from tumorous hamsters. The tumorlike (T) antigen in infected cells was found to be smaller than the viral structural antigens and, therefore, could be separated from them by centrifugation in a linear sucrose gradient. Investigation of the production of the T antigen in virus-infected cells further distinguished it from viral structural proteins by the following properties: (i) the T antigen was first detected 3 to 4 hr after infection, whereas viral antigens were synthesized 17 to 20 hr after infection; and (ii) the T antigen was produced when deoxyribonucleic acid (DNA) biosynthesis was inhibited by 5-fluorodeoxyuridine (10(-6)m), but viral proteins were not synthesized in the absence of viral DNA replication.     

Metabolites ofStreptomyces noursei

Было установлено, что актиномицет Str. noursei образует амиды некоторых разветвленных алифатических кислот: изобутирамид, метилэтилацетамид, изовалерамид, а, вероятно, и более сложные гомологи, как изокапронамид, изоэнантамид и 4-метилкапронамид. Обсуждается биогенез этих веществ.