全文获取类型
收费全文 | 127253篇 |
免费 | 22061篇 |
国内免费 | 8540篇 |
专业分类
157854篇 |
出版年
2024年 | 267篇 |
2023年 | 1482篇 |
2022年 | 3382篇 |
2021年 | 5768篇 |
2020年 | 5519篇 |
2019年 | 7822篇 |
2018年 | 7780篇 |
2017年 | 6975篇 |
2016年 | 8240篇 |
2015年 | 10209篇 |
2014年 | 11144篇 |
2013年 | 12000篇 |
2012年 | 11247篇 |
2011年 | 10111篇 |
2010年 | 8086篇 |
2009年 | 6522篇 |
2008年 | 6216篇 |
2007年 | 5102篇 |
2006年 | 4424篇 |
2005年 | 3651篇 |
2004年 | 3031篇 |
2003年 | 2796篇 |
2002年 | 2334篇 |
2001年 | 1919篇 |
2000年 | 1694篇 |
1999年 | 1688篇 |
1998年 | 956篇 |
1997年 | 974篇 |
1996年 | 890篇 |
1995年 | 825篇 |
1994年 | 742篇 |
1993年 | 606篇 |
1992年 | 720篇 |
1991年 | 560篇 |
1990年 | 474篇 |
1989年 | 346篇 |
1988年 | 286篇 |
1987年 | 239篇 |
1986年 | 195篇 |
1985年 | 217篇 |
1984年 | 130篇 |
1983年 | 123篇 |
1982年 | 60篇 |
1981年 | 30篇 |
1980年 | 21篇 |
1979年 | 23篇 |
1976年 | 4篇 |
1973年 | 3篇 |
1972年 | 4篇 |
1965年 | 2篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
91.
92.
93.
Myosin associated with the male germ cells of angiosperms interacts with actin, promoting transport of the non-motile generative
and later sperm cells in the pollen tube. Myosin localizing on the sperm cell plasma membrane seems negligible in Plumbago, as reflected by the absence of: (i) anti-myosin labeling using immunoelectron microscopy, (ii) sperm motility on actin matrices,
and (iii) electrophoretic movement changes after addition of antibody. Sperm cells injected directly into actively streaming
Nitella internodal cells, however, follow actin bundles and their movement is sensitive to ATP and Mg2+. This may be based on simple charge binding since negatively charged latex beads also migrate on actin, whereas neutral or
positively-charged latex beads do not. Sperm cells are negatively charged according to capillary microelectrophoresis, whereas
killed sperm cells, which are positively charged do not migrate. The sperm cell that normally fertilizes the egg has a higher
calculated charge (8.277 × 103 esu/cm2) compared with the sperm cell that fuses with the central cell (6.120 × 103 esu/cm2).
Received: 15 December 1998 / Accepted: 21 January 1999 相似文献
94.
95.
Distinct effects of climate warming on populations of silver fir (Abies alba) across Europe 下载免费PDF全文
96.
97.
Despite intense study by physicists and biologists, we do not fully understand the unsteady aerodynamics that relate insect wing morphology and kinematics to lift generation. Here, we formulate a force partitioning method (FPM) and implement it within a computational fluid dynamic model to provide an unambiguous and physically insightful division of aerodynamic force into components associated with wing kinematics, vorticity, and viscosity. Application of the FPM to hawkmoth and fruit fly flight shows that the leading-edge vortex is the dominant mechanism for lift generation for both these insects and contributes between 72–85% of the net lift. However, there is another, previously unidentified mechanism, the centripetal acceleration reaction, which generates up to 17% of the net lift. The centripetal acceleration reaction is similar to the classical inviscid added-mass in that it depends only on the kinematics (i.e. accelerations) of the body, but is different in that it requires the satisfaction of the no-slip condition, and a combination of tangential motion and rotation of the wing surface. Furthermore, the classical added-mass force is identically zero for cyclic motion but this is not true of the centripetal acceleration reaction. Furthermore, unlike the lift due to vorticity, centripetal acceleration reaction lift is insensitive to Reynolds number and to environmental flow perturbations, making it an important contributor to insect flight stability and miniaturization. This force mechanism also has broad implications for flow-induced deformation and vibration, underwater locomotion and flows involving bubbles and droplets. 相似文献
98.
99.
Jiaohong Zhao Fudan Gao Jingsong Zhang Teruo Ogawa Weimin Ma 《The Journal of biological chemistry》2014,289(39):26669-26676
Two mutants that grew faster than the wild-type (WT) strain under high light conditions were isolated from Synechocystis sp. strain PCC 6803 transformed with a transposon-bearing library. Both mutants had a tag in ssl1690 encoding NdhO. Deletion of ndhO increased the activity of NADPH dehydrogenase (NDH-1)-dependent cyclic electron transport around photosystem I (NDH-CET), while overexpression decreased the activity. Although deletion and overexpression of ndhO did not have significant effects on the amount of other subunits such as NdhH, NdhI, NdhK, and NdhM in the cells, the amount of these subunits in the medium size NDH-1 (NDH-1M) complex was higher in the ndhO-deletion mutant and much lower in the overexpression strain than in the WT. NdhO strongly interacts with NdhI and NdhK but not with other subunits. NdhI interacts with NdhK and the interaction was blocked by NdhO. The blocking may destabilize the NDH-1M complex and repress the NDH-CET activity. When cells were transferred from growth light to high light, the amounts of NdhI and NdhK increased without significant change in the amount of NdhO, thus decreasing the relative amount of NdhO. This might have decreased the blocking, thereby stabilizing the NDH-1M complex and increasing the NDH-CET activity under high light conditions. 相似文献
100.
Localized reversible frameshift mutation in an adhesin gene confers a phase-variable adherence phenotype in mycoplasma 总被引:13,自引:0,他引:13
The variable adherence-associated (Vaa) antigen of Mycoplasma hominis is an abundant surface lipoprotein adhesin that may mediate important interactions of this wall-less prokaryotic pathogen with the human host. Extensive mutational variation of Vaa size, as well as sequence and antigenic divergence, has been described previously. Using a series of clonal isolates representing an isogenic lineage of variants oscillating in Vaa expression, Vaa is further shown in this study to undergo high-frequency phase variation in expression, which correlated precisely with the ability of M . hominis to adhere to cultured human cells. Although no DNA rearrangements or sequence differences in the 5' regions flanking vaa alleles were detected between Vaa+ and Vaa− variants, intragenic vaa sequences from this lineage revealed an oscillating mutation involving a single nucleotide deletion/insertion in a short tract of adenine residues near the 5' end of the mature Vaa coding sequence, which created a translational frameshift resulting in either a complete Vaa ORF or an in-frame UAG stop codon immediately downstream of the poly-A tract. Evidence for the occurrence of this high-frequency frameshift mutation in vivo was obtained from analysis of PCR-generated vaa sequences amplified from the joint synovial fluid of a patient with M . hominis -associated arthritis, which indicated that Vaa phase variation occurs during M . hominis infection in the natural host. These results identify a distinctive frameshift mutator element in the vaa gene that governs M . hominis adherence and highlight the importance of mutational alteration of primary gene products on the mycoplasma surface as a means of generating and maintaining functional diversity in the host. 相似文献