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81.
Meiheng Yang Howard Allen Hisao Fukushima Richard A. DiCioccio 《Glycoconjugate journal》1984,1(1):15-19
Fucosidosis is an autosomal recessive lysosomal storage disease resulting from the absence of -l-fucosidase activity. Two natural missense mutations (G197A) and (A860G) within the -l-fucosidase gene have been reported to be homozygous in four patients with fucosidosis. Expression of wild-type and mutated -l-fucosidase cDNAs in COS-1 cells revealed complete deficiency of -l-fucosidase for the G197A transition and a normal level of enzyme for A860G. We therefore conclude that the change of G197A is responsible for fucosidosis in the patients while A860G is a normal polymorphic variant of -l-fucosidase. 相似文献
82.
Factors affecting septation (cell division) of the tendril whichfacilitates the organic connection with the host were studiedin a root parasite Aeginetia indica L. Transverse cell division,which occurs perpendicular to the long axis of the tendril,was promoted by additions of sucrose, glucose and cytokininsto the basal medium. Longitudinal cell division of the tendril,which takes place parallel or obliquely to the long axis, wasstimulated by cytokinins, but not by sucrose. The latter typeof cell division was frequent in basal and sub-basal cells ofthe tendril but was extremely rare in apical cells. The orientationof the planes of these cell divisions was closely related tocell shape. Abnormal growth of the tendril was seen in germinatingseeds grown for six weeks or more in media containing both Miscanthus(a host) root extract and cytokinin. (Received February 23, 1984; Accepted June 12, 1984) 相似文献
83.
An enzyme that catalyzes the formation of 6-(D-erythro-1',2',3'-trihydroxypropyl)-7,8-dihydropterin triphosphate (D-erythrodihydroneopterin triphosphate) and formic acid from GTP has been purified about 3700-fold from homogenates of chicken liver. The molecular weight of the enzyme, D-erythrodihydroneopterin triphosphate synthetase (GTP cyclohydrolase), has been estimated to be 125,000 by gel filtration on Ultrogel AcA-34. The enzyme functions optimally between pH 8.0 and 9.2 and is considerably heat-stable. No cofactors or metal ions have been demonstrated to be required for activity; however, the reaction is strongly inhibited by Cu2+ and Hg2+. GTP is the most efficient substrate, with GDP being 1/17 as active and guanosine, GMP, and ATP being inactive. The Km for GTP has been found to be 14 micrometer. Although the overall reaction catalyzed by D-erythrodihydroneopterin triphosphate synthetase from chicken liver is identical with that from Escherichia coli GTP cyclohydrolase, immunological studies show no apparent homology between the two enzymes. 相似文献
84.
H Okayama C M Edson M Fukushima K Ueda O Hayaishi 《The Journal of biological chemistry》1977,252(20):7000-7005
Poly(ADP-ribose) synthetase has been purified approximately 5000-fold from rat liver nuclei. The activity of the purified enzyme is absolutely dependent upon the presence of native or synthetic DNA, and the further addition of histone(s) stimulates the activity 3- to 5-fold. When the ADP-ribosylated material synthesized in the absence or presence of various histones is analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, the major product in all cases migrates between histones H1 and H3-H2B with the same RF value of 0.58 relative to the marker dye. No ADP-ribose was found to co-electrophorese with any of thehistones. The addition of histones does not affect the chain number of the poly(ADP-ribose) synthesized but does result in an increase in the average chain length of the polymer. In the presence of histones, the Km for NAD+ decreases from 80 micron to 25 micron and the Vmax doubles. These results indicate that, in the purified poly(ADP-ribose) synthetase system, histones are not ADP-robosylated but act as allosteric activators. 相似文献
85.
Masanori Fukushima Taketoshi Kato Ryuzo Ueda Kazuo Ota Shuh Narumiya Osamu Hayaishi 《Biochemical and biophysical research communications》1982,105(3):956-964
Cytotoxic actions of various prostaglandins were examined on L1210 mouse leukemia and several human leukemia cell lines, and prostaglandin D2 (PGD2) was found most active. PGD2 exerted a dose dependent inhibition of L1210 cell growth over 3.6 μ. At 14.3 μ growth was completely inhibited, and the number of viable cells remarkably decreased during culture. Microscopically the remaining cells showed degenerative changes with many vacuoles in their cytoplasm. The IC50 value of PGD2 on L1210 cell growth was calculated to be 6.9 μ (2.4 μg/ml), and at this concentration the DNA synthesis in 24 hr cultured cells was also decreased to a half of the level in the control cells. Such growth inhibition by PGD2 was also found at similar concentrations with several human leukemia cell lines such as NALL-1, RPMI-8226, RPMI-8402, and Sk-Ly-16. Among other prostaglandins tested, PGA2 showed a comparable, and PGE2 a less but significant growth inhibitory activity, while PGB2, PGF2α and PGI2 had no such effects on cell proliferation at 14.3 μ concentration. These results suggest a potential antineoplastic activity of PGD2. 相似文献
86.
Hirofumi Fukushima Shigenobu Umeki Takehito Watanabe Yoshinori Nozawa 《Biochemical and biophysical research communications》1982,105(2):502-508
With the use of detergents and successive column chromatographies, Tetrahymena b-type cytochrome was purified from microsomes to a specific content of 36.0 nmol per mg of protein. The purified form showed a single band on SDS-polyacrylamide gel with molecular weight of 22,000. The spectral properties of the reduced b-type cytochrome, the α-peak of which is situated at 560 nm and asymmetric with a shoulder at 556 nm, was different from that of rat liver microsomal cytochrome b5. However, it was reducible by NADH in the presence of NADH-cytochrome b5 reductase purified from rat liver microsomes.The results indicated that the microsomal b-type cytochrome should be designated as cytochrome b5 of a ciliated protozoan, Tetrahymena pyriformis. 相似文献
87.
O Fukushima T Yamamoto C V Gay 《The journal of histochemistry and cytochemistry》1991,39(9):1207-1213
Using lead citrate as a capture reagent and adenylate-(beta, gamma-methylene) diphosphate (AMP-PCP) as a substrate, we localized adenylate cyclase activity on the non-ruffled border plasma membrane of approximately half of the osteoclasts on trabecular bone surfaces in the tibial metaphyses of chickens fed a low (0.3%)-calcium diet. The enzyme was not detectable in osteoclasts when chickens were fed a normal calcium diet. Activity was observed on the entire plasma membrane of detached osteoclasts that were situated between osteoblasts on the bone surface and blood vessels in the marrow cavity. Detection of activity on detached osteoclasts required the presence of an activator, implying lower levels in these cells than in those with ruffled borders. Staining was greater on the lateral sides of osteoblasts and osteoclasts when they were in contact with each other. Reaction specificity was indicated by the demonstration of stimulation by forskolin, guanylate-(beta, gamma-methylene) diphosphate (GMP-PCP), dimethylsulfoxide, and NaF, inhibition by alloxan and 2',5'-dideoxyadenosine, and absence of activity when sections were incubated in substrate-free medium or when GMP-PCP replaced AMP-PCP as a substrate. The finding of adenylate cyclase in osteoclast plasma membrane provides structural evidence that the adenylate cyclase-cyclic AMP system has a role in regulation of osteoclast cell function. The low-calcium diet appears to have resulted in increased amounts of adenylate cyclase in osteoclasts. 相似文献
88.
There has been interest in the phenomenon that a cell cannot undergo unlimited reproduction under adequate conditions and undergoes senescence. In holotrichous ciliates, Paramecium has a limit of vegetative reproduction without sexual reproduction but Tetrahymena does not always have a limited lifespan. Comparing the two species would increase our knowledge of the mechanism of cellular clonal aging. We previously showed that mutations induced by X-rays shorten clonal lifespan. In this study, we examined whether mutagens shorten the clonal lifespan of Paramecium tetraurelia. P. tetraurelia was exposed to the alkylating agent N-methyl-N'-nitro-N-nitrosoguanidine (MNNG), 0.045 mg/ml, for 30 min. The animal was exposed to MNNG 6 times in total while young (under 80 divisions from the start of a clonal life cycle) or 4 times during the senescent stage. MNNG shortened the clonal lifespan as expressed by the decrease in fission number from 186 +/- 55 (4 cell lines) to 136 +/- 21 (6 cell lines) with the first two treatments but with further exposures the lifespan increased to 182 +/- 15 (5 cell lines). MNNG had no effect when administered at the older age. Exposure of P. tetraurelia to 4-nitroquinoline-N-oxide at 0.021 mg/ml twice for 12 and 15 min at the younger age reduced the mean clonal lifespan from 143 +/- 28 to 125 +/- 21 and the maximum lifespan from 263 +/- 33 to 175 +/- 25. 相似文献
89.
Kazuhiro Nakamura Yasuhiro Goto Naoko Yoshie Yoshio Inoue 《International journal of biological macromolecules》1992,14(6):321-325
It was found that an optically active copolyester, poly(3-hydroxybutyrate-co-3-hydroxyvalerate), denoted as P(3HB-co-3HV), is synthesized by Alcaligenes eutrophus H16 from several amino acids under various fermentation conditions. The optimum condition for the biosynthesis from one amino acid, threonine, was investigated and its biosynthetic pathway was discussed on the basis of the relation between the fermentation condition and the co-monomer composition of the produced polyesters. 相似文献
90.
YM175, a new bisphosphonate, increases serum 1,25-dihydroxyvitamin D in rats via stimulating renal 1-hydroxylase activity 总被引:1,自引:0,他引:1
Y Nagao Y Ishitobi H Kinoshita S Fukushima H Kawashima 《Biochemical and biophysical research communications》1991,180(3):1172-1178
Effect of YM175, a new bisphosphonate, on vitamin D metabolism was studied in rats. When animals were treated with the compound, serum 1,25-dihydroxyvitamin D increased in a dose dependent manner. The effect was also detected in thyroparathyroidectomized animals. The effect appears to be due to the stimulation of renal production of the hormone, since renal 1-hydroxylase was also elevated in these animals. However, when kidneys were incubated with YM175 and then renal 1-hydroxylase activity was examined, the enzyme activity was not different from that of non-treated control kidney. We conclude therefore that YM175 indirectly stimulates renal 25-hydroxyvitamin D-1-hydroxylase by increasing circulating parathyroid hormone via an unknown mechanism independent of parathyroid hormone. This is the first direct demonstration of increase in the renal production of 1,25-dihydroxyvitamin D resulting from bisphosphonate treatment. 相似文献