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排序方式: 共有227条查询结果,搜索用时 140 毫秒
221.
222.
N Benhamou N Gilboa-Garber J Trudel A Asselin 《The journal of histochemistry and cytochemistry》1988,36(11):1403-1411
We report the development of a cytochemical affinity technique for detection of galacturonic acids at the ultrastructural level. The highly purified gonad lectin from Aplysia depilans (AGL) was tagged with colloidal gold particles and used for labeling carbohydrates in resin-embedded sections of various plant and fungal tissues. Patterns of AGL binding sites were compared to those obtained with a D-galactose-specific lectin, Ricinus communis agglutinin I. Differences in labeling patterns were noted, indicating that the lectins exhibited differential carbohydrate binding. In addition, the considerable loss of labeling over isolated wheat coleoptile walls treated for removal of pectin, after incubation with the AGL-gold complex, strongly suggested an affinity of AGL for pectic substances. A series of cytochemical controls, including sugar inhibition tests, has proven the specificity of the technique and the high affinity of AGL towards galacturonic acids. The potential value of this new lectin for ultrastructural studies on cell wall pectic substances in plant biology and pathology is demonstrated. 相似文献
223.
This study evaluated the effects of gestation and lactation on stomach growth in rats. Constant increases were observed in gastric fundus weight during pregnancy and lactation, with a maximum obtained after 3 weeks of lactation. This increase in tissue weight was not associated with hyperplasia of the gastric fundus as in no instance did total DNA contents from mated females differ significantly from those of the unmated controls. Mild hypertrophy, as evidenced by significant increases in cellular mass and RNA and protein concentrations, was observed during late lactation. Gastrin might be responsible for these modest changes in the gastric fundus. 相似文献
224.
Jean-Christophe Giard Axel Hartke Sigrid Flahaut Abdellah Benachour Philippe Boutibonnes Yanick Auffray 《Current microbiology》1996,32(5):264-271
Compared with growing bacteria, carbohydrate-starved cells of Enterococcus faecalis show development of a multiresistance state against heat, H2O2, acid, and ethanol, but not against UV irradiation. The kinetics of acquisition of resistance is different according to the
stress. Three hours of starvation provide maximal resistance against ethanol, while the tolerance to heat, H2O2, and acid increases progressively with the duration of starvation. Chloramphenicol treatment does not abolish the ethanol
tolerance. Protein synthesis inhibition during the transitional growth phase and the first hours of starvation partially inhibit
the acquisition of heat and oxidative resistances. Antibiotic treatment after 3 h of starvation does not affect the increase
of these resistances. We suggest that synthesis of specific proteins revealed by 2-D gel analysis in the first 3 h of starvation,
followed by a second mechanism related to protein degradation or alteration, is necessary for acquisition of maximal resistance
towards heat and oxidative stresses. 相似文献
225.
226.
Axel Hartke Sandrine Bouché Jean-Christophe Giard Abdellah Benachour Philippe Boutibonnes Yanick Auffray 《Current microbiology》1996,33(3):194-199
The lactic acid tolerance response (LATR) of the lactic acid bacterium Lactococcus lactis subsp. lactis has been studied. A dramatic increase in survival to a severe acid stress (pH 3.9) was obtained by preexposing the cells
for 30 min to a mildly acid shock at pH 5.5. Whole-cell protein extract analysis revealed that during the acid tolerance response
33 polypeptides are induced over the level of naive cells. Among these are the major heat shock proteins DnaK and GroEL. In
conjunction with a previous report (Hartke et al. 1994), the results establish that L. lactis can adapt to lactic acid exposure in two different ways: a logarithmic phase LATR, which may be activated by protons, and
a stationary-phase LATR, which needs no activation by protons. Both systems are independent of de novo protein synthesis.
Received: 8 February 1996 / Accepted: 11 March 1996 相似文献
227.