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BYUNG CHEOL CHO JONG SOO PARK KUIDONG XU JOONG KI CHOI 《The Journal of eukaryotic microbiology》2008,55(5):417-426
ABSTRACT. A new ciliate, Trimyema koreanum n. sp., isolated from hypersaline water (salinity of 293‰) from a solar saltern in Korea, was investigated using live observation, protargol impregnation, and gene sequencing. Trimyema koreanum is about 30 × 13 μm in vivo, has usually 23 longitudinal ciliary rows forming two distinct ciliary girdles visible both in vivo and in protargol impregnation. A third indistinct ciliary girdle as well as a girdle of mucocysts is distinguishable only in impregnated cells. We suggest T. koreanum as a new species, differing from the most similar species, T. marinum, by the presence of two distinct ciliary girdles (T. marinum usually has six ciliary girdles clearly visible in living cells and three anterior spirals that encircle the cell completely). Although the number of known 18S rRNA sequences in the genus Trimyema was limited, the Trimyema group including T. koreanum forms a strong clade. The phylogenetic position confirms that the isolate belongs to the genus Trimyema and is different from previously sequenced species. Trimyema koreanum is able to consume both prokaryotes and small eukaryotes (specifically, the alga Dunaliella sp.). 相似文献
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HIROSHI IKEDA KOHEI KUBOTA YOUNG-BOK CHO HONGBIN LIANG TEIJI SOTA 《Biological journal of the Linnean Society. Linnean Society of London》2009,98(2):452-467
To reveal differences in phylogeographic patterns of flightless insect species occurring in different regions of Japan, we studied the phylogeography and demographic history of Silpha beetles occurring in cool-temperate habitats of two major islands, Honshu and Hokkaido, using sequences of the mitochondrial cytochrome oxidase subunit I (COI) gene. Honshu has a more mountainous topography, and cool-temperate habitats occur discontinuously, whereas Hokkaido, located to the north of Honshu, has more continuous cool-temperate habitats. A species endemic to Honshu, S. longicornis occurs on Honshu, whereas S. perforata occurs on Hokkaido and the East Asian continent. Our results indicate that the ancestors of S. longicornis colonized Honshu via a south-west route c . 0.7 Mya and the species has highly divergent populations in isolated mountainous areas of Honshu, whereas S. perforata colonized Hokkaido via a northern route less than 90 000 years ago and has less divergent geographic populations. During the last glacial period, S. perforata was probably restricted to refugia in southern Hokkaido and later expanded into northern Hokkaido, whereas S. longicornis populations existed in many isolated refugia, probably because of the complex topography of Honshu. Thus, our study demonstrates that, even between closely related species, interactions among biology, latitudinal climatic gradients and topography can produce different phylogeographic patterns. © 2009 The Linnean Society of London, Biological Journal of the Linnean Society , 2009, 98 , 452–467. 相似文献
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Seven years of recent European net terrestrial carbon dioxide exchange constrained by atmospheric observations 总被引:1,自引:0,他引:1
W. PETERS M. C. KROL G. R. Van Der WERF S. HOUWELING C. D. JONES J. HUGHES K. SCHAEFER K. A. MASARIE A. R. JACOBSON J. B. MILLER C. H. CHO M. RAMONET M. SCHMIDT L. CIATTAGLIA F. APADULA D. HELTAI F. MEINHARDT A. G. DI SARRA S. PIACENTINO D. SFERLAZZO T. AALTO J. HATAKKA J. STRÖM L. HASZPRA H. A. J. MEIJER S. VAN DER LAAN R. E. M. NEUBERT A. JORDAN X. RODÓ J.‐A. MORGUÍ A. T. VERMEULEN E. POPA K. ROZANSKI M. ZIMNOCH A. C. MANNING M. LEUENBERGER C. UGLIETTI A. J. DOLMAN P. CIAIS M. HEIMANN P. P. TANS 《Global Change Biology》2010,16(4):1317-1337
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KYUNG‐HO MA KWANG‐HO KIM ANUPAM DIXIT JAE‐WOONG YU JONG‐WOOK CHUNG JEONG‐HEUI LEE EUN‐GI CHO TAE SAN KIM YONG‐JIN PARK 《Molecular ecology resources》2006,6(3):689-691
A microsatellite‐enriched library of Job's tears (Coix lacryma‐jobi L. var. Ma‐yuen Stapf) was constructed using a modified biotin–streptavidin capture method. After screening, 17 polymorphic microsatellites were used for diversity analysis among 30 Job's tears accessions. The number of alleles ranged from one to five alleles per locus with an average of 2.8 alleles. Expected heterozygosity (HE) and polymorphism information content (PIC) ranged from 0 to 0.676 and from 0 to 0.666, respectively. The newly developed microsatellite markers are expected to be very valuable tools for evaluation of genetic diversity among large germplasm collection of Job's tears present in our Korean Gene Bank. 相似文献
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Two quediine species (Quedius samuraicus Bernhauer and Schubert, and Velleius elongatus Naomi) were identified for the first time in Korea. Diagnoses and illustrations of the habitus and male genitalia are presented. 相似文献
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Hipparchia autonoe, designated Natural Monument no. 458, is a species inhabiting Mt. Halla on Jeju Island, which is the only habitat of H. autonoe in South Korea. Recently, this species is in danger of extinction as the population has been remarkably reduced because its host plants and nectar plants are gradually disappearing due to plant succession in its habitat. On the climbing road of Jeju Island Eorimok, H. autonoe used to be observed at and above 1300 m a.s.l. However, since Sasa quelpaertensis has recently expanded its habitat up to 1400 m, H. autonoe is now observed at and above 1500 m. In Mongolia, the population of H. autonoe seems quite stable as there are dense populations of a host plant and nectar plants. Accordingly it is judged that we can maintain a stable community for support of H. autonoe if we successfully manage the host plant and nectar plant communities by controlling the density of S. quelpaertensis using biological and physical control methods. Comparative analysis of DNA barcode region of COI (658 bp) was done to check the homogeneity and the genetic diversity of H. autonoe collected in Korea and Mongolia. The DNA sequence difference among individuals collected in South Korea was 0.0–0.2%, and in Mongolia 0.0–1.4%. This confirmed a 0.06–1.2% barcoding gap. We believe that this result will provide basic information useful to guide conservation of H. autonoe in South Korea. 相似文献
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Adipose tissue-derived stem cells (ADSCs) are one population of adult stem cells that can self renew and differentiate into
multiple lineages. Because of advantages in method and quantity of acquisition, ADSCs are gaining attention as an alternative
source of bone marrow mesenchymal stem cells. In this study, we performed microRNA profiling of undifferentiated and of neurally-differentiated
ADSCs to identify the responsible microRNAs in neurogenesis using this type of stem cell. MicroRNAs from four different donors
were analysed by microarray. Compared to the undifferentiation control, we identified 39–101 microRNAs with more than two-fold
higher expression and 3–9 microRNAs with two-fold lower expression. The identified microRNAs were further analysed in terms
of gene ontology (GO) in relation with neurogenesis, based on their target mRNAs predicted by computational analysis. This
study revealed the specific microRNAs involved in neurogenesis via microRNA microarray, and may provide the basic information
for genetic induction of adult stem cell differentiation using microRNAs. 相似文献