水稻黄单胞菌水稻致病变种的超氧化物歧化酶活性及诱导

以水稻黄单胞菌水稻致病变种(Xanthomonas oryzae pv.oryzae)的毒性菌株PXO99Ａ和无毒菌株PXO99A(pBUavrXa10F1),检测液体培养中菌体超氧化物歧化酶(SOD)活性变化,以说明SOD与菌株致病性的关系。结果表明,菌体SOD活性高峰出现于延迟期末,之后下降;毒性菌株SOD活性高于无毒菌株。两个菌株的SOD活性的胞内定位均以胞质为主,占总活性的70%以上;酶体周质中SOD活性占总活性的20%～30%。以50～800μmol/L外源O-2处理细菌培养物1?h,可诱导菌体中SOD活性的增加。其 中以200?μmol/L O-2处理SOD活性最高;12?h菌龄培养物的诱导效果优于24?h培养物;对毒性菌株SOD的诱导作用更为明显。外源O-2处理后细菌存活率明显降低,2 4?h培养物的存活率下降大于12?h培养物;毒性菌株存活率下降大于无毒菌株。     

Correction to: Development of an autotrophic fermentation technique for the production of fatty acids using an engineered Ralstonia eutropha cell factory

Journal of Industrial Microbiology & Biotechnology - Massive emission of CO2 into atmosphere from consumption of carbon deposit is causing climate change. Researchers have applied metabolic...     

The deposition and significance of an Ediacaran non-glacial iron formation

Most Neoproterozoic iron formations (NIF) are closely associated with global or near-global “Snowball Earth” glaciations. Increasingly, however, studies indicate that some NIFs show no robust evidence of glacial association. Many aspects of non-glacial NIF genesis, including the paleo-environmental setting, Fe(II) source, and oxidation mechanisms, are poorly understood. Here, we present a detailed case study of the Jiapigou NIF, a major non-glacial NIF within a Neoproterozoic volcano-sedimentary sequence in North Qilian, northwestern China. New U–Pb geochronological data place the depositional age of the Jiapigou NIF at ~600 Ma. Petrographic and geochemical evidence supports its identification as a primary chemical sediment with significant detrital input. Major and trace element concentrations, REE + Y systematics, and ε_Nd(t) values indicate that iron was sourced from mixed seawater and hydrothermal fluids. Iron isotopic values (δ⁵⁶Fe = −0.04‰–1.43‰) are indicative of partial oxidation of an Fe(II) reservoir. We infer that the Jiapigou NIF was deposited in a redox stratified water column, where hydrothermally sourced Fe(II)-rich fluids underwent oxidation under suboxic conditions. Lastly, the Jiapigou NIF has strong phosphorous enrichments, which in other iron formations are typically interpreted as signals for high marine phosphate concentrations. This suggests that oceanic phosphorus concentrations could have been enriched throughout the Neoproterozoic, as opposed to simply during glacial intervals.     

Synergistic effects between [Si-hemicellulose matrix] ligands and Zn ions in inhibiting Cd ion uptake in rice (Oryza sativa) cells

Planta - Our study demonstrated that Zn alleviated Cd toxicity in the presence of Si in the cell walls by Zn 2+ binding to ligands through the formation of the [Si-hemicellulose matrix]Zn complexes...     

The diagnostic and prognostic value of pseudogene SIGLEC17P in lung adenocarcinoma and a preliminary functional study

Among malignant tumors, lung adenocarcinoma (LUAD) is the leading cause of death worldwide. This study explored the diagnostic, prognostic value, and preliminary functional verification of sialic acid binding Ig like lectin 17, pseudogene (SIGLEC17P) in LUAD. Prognostic lncRNAs for LUAD were identified by The Cancer Genome Atlas and quantitative real-time PCR (qRT-PCR) was used to detect the expression of SIGLEC17P in LUAD and paracarcinoma tissues. Subsequently, lentiviral vectors were used to overexpress SIGLEC17P in A549 and H1299 cells. The effects of SIGLEC17P overexpression on the proliferation, migration, and invasiveness of LUAD cells (A549 and H1299) were evaluated by Cell Counting Kit-8, wound healing, and transwell migration assays, respectively. Bioinformatics analyses were performed to reveal the potential pathways in which SIGLEC17P is involved in LUAD. qRT-PCR results revealed low SIGLEC17P expression in LUAD tissues and a significant association with the N stage, T stage, and tumor node metastasis stage. Furthermore, the receiver operating characteristic curve demonstrated a reliable diagnostic value. The proliferation, migration, and invasion of LUAD cells were inhibited by overexpression of SIGLEC17P. Bioinformatics analyses suggested that SIGLEC17P might exert antioncogenic effects in LUAD through the mir-20-3p/ADH1B or mir-4476-5p/DPYSL axis. In summary, our results revealed that SIGLEC17P acts as a prognostic biomarker, independent prognostic factor, and potential therapeutic target for patients with LUAD.