亚洲象无齿奎隆线虫一新种：（圆形亚目：毛线科）

本文报道作者近年来在石家庄动物园对亚洲象Elephas maximus进行药物驱虫时采集的寄生线虫一新种,无齿奎隆线虫,新种Quilonia edentata sp.nov.,模式标本保存于河北师范大学生物系。     

辐射增敏剂甲硝唑氨酸对坪期V_(79)细胞DNase活力的影响

研究了辐射增敏剂甲硝唑氨酸(CM)对受照前后坪期V_(79)细胞DNase活力的影响。结果表明CM在一定浓度范围内(1—10mmol/L)对DNase的激活作用有浓度依赖关系。细胞经6—12Gyγ线照后DNase活力亦增高,若照射合并CM处理后,则对DNase激活有相加作用。还就DNase活力升高与DNA链断裂间的可能关系进行了讨论。     

流行性感冒病毒重组株京生75-29 R2 T1及冷适应株31-广的基因分析

用聚丙烯酰胺凝胶电泳方法分析了流行性感冒病毒重组株京生75-29R2 T1(H3N2)及冷适应株31-广(H3N2)的RNA及多肽。重组株京生75-29R2 T1的HA及M基因系来自流行病毒亲本株/甲/北京/29/75(H3N2),而P_2、NA、NP及NS基因则来自温度敏感母株福R3(H2N2)。流行病毒株甲/穗/03/68(H3N2)在低温条件下经鸡胚尿囊腔传递24代而获得的冷适应疫苗毒株31-广(H3N2)其基因型与野毒株一致。     

二十二种木材对家白蚁天然抗性的室内测定

白蚁蛀蚀不同树种的木材有明显的偏爱性,各种木材抵抗白蚁的蛀蚀能力也有很大差异。如在我国南方,用马尾松作房屋中的木构件,短短几年内就可能遭受白蚁蛀损,而某些抗蚁蛀良好的木材,使用年限超过百年的却不乏其例。研究太材对白蚁的天然抗性能为选择造林和绿化树种,合理用材以及寻找植物性抗白蚁物质提供依据。本工作对广东常见的二十二种本地产和外来树种的木材,用南方危害最大的家白蚁Coptotermes formosanus Shiraki作室内生物测定,并讨论了影响木材抗蚁蛀性的各种因素。     

甲型肝炎病毒对理化因子的稳定性

用放射免疫成斑法研究了甲型肝炎病毒FM-175株的理化稳定性和灭活条件,证明甲型肝炎病毒理化稳定性与其它肠道病毒相似,具有广范围的pH(2～10)稳定性;Mg~(++)和Ca可增强其热稳定性,不能抵抗冷冻干燥,但对热的抵抗力明显高于普通肠道病毒,可被紫外线迅速杀灭,也可被多种消毒剂如3～8％的甲醛液,50～90％的乙醇,2％的石炭酸及400ppm的有效氯等杀灭,但可抵抗0.1％甲醛液,2～5％的来苏儿及200ppm的有效氯1小时以上,本研究工作为甲型肝炎病毒的理化性状、保存及消毒条件等提供了实验数据。     

应用酶联免疫吸附试验检测马铃薯卷叶病毒

以辣根过氧化物酶标记马铃薯卷叶病毒抗体,采用双抗体夹心ELISA方法鉴定了马铃薯和洋酸浆的茎、叶、根及马铃薯块茎中的马铃薯卷叶病毒(Potato Leafroll Virus,PLRV),结果表明,对提纯的PLRV可测出的最低浓度为25ng/ml,当包被抗体浓度为40μg/ml、酶标记抗体稀释度为1/120时,可测出马铃薯茎、叶和根汁液中的PLRV,感染PLRV的洋酸浆茎、叶和根汁液的消光值,均比无病对照者高二倍以上,虽然感染PLRV的马铃薯休眠块茎维管束组织汁液的消光值高于无病毒对照,且脐部维管束组织消光值高于顶端,但测定打破休眠的感病块茎顶端维管束组织的阳性结果更为可靠和明显。     

Cell-mediated and humoral immune responses to aspermatogenic antigen in experimental allergic orchitis in the guinea-pig

Guinea-pigs were immunized with a defined and highly potent aspermatogenic antigen, G75m, and the occurrence of orchitis was correlated with (1) cell-mediated immune response to G75m, determined by lymph node cell proliferation and by secretion of macrophage migration inhibitory factor (MIF) by peritoneal exudate cells, and (2) humoral antibodies to G75m and to cell surface antigens of guinea-pig testicular cells, by radioimmunometric assays. A consistent temporal relationship between cell-mediated immune responses and disease was found: lymph node cell proliferation was positive by Day 4, followed 3 days later by maximum secretion of MIF, and orchitis lesions were manifest on Day 10. In contrast, maximal IgG antibodies to G75m or to the surface antigens of spermatozoa/testicular cells were detected at a time when cell-mediated immune responses and active testicular lesions had subsided. In individual animals, lymph node cell proliferation increased with severity of orchitis, while MIF secretion by peritoneal cells increased with orchitis only late in the disease. Early in disease, MIF response showed a negative correlation with orchitis. Moreover, peritoneal injection of oil reduced the incidence of early lymph node cell proliferative responses, and delayed the onset of testicular disease. These findings are consistent with competition between different inflammatory sites for recently antigen-activated T lymphocytes. We conclude that (1) the development of orchitis correlates with cell-mediated immune responses to purified aspermatogenic antigens but not with IgG antibody responses, and (2) when the same animal is used to assess different aspects of cellular immunity and autoimmune disease, one study may significantly influence the other.