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111.
112.
Talmor M FAhey TJ Wise J Hoffman LA Barie PS 《Plastic and reconstructive surgery》2000,105(6):2244-8; discussion 2249-50
Large-volume liposuction can be associated rarely with major medical complications and death. The case of exsanguinating retroperitoneal hemorrhage that led to cardiopulmonary arrest in an obese 47-year-old woman who underwent large-volume liposuction is described. Extensive liposuction is not a minor procedure. Performance in an ambulatory setting should be monitored carefully, if it is performed at all. Reporting of adverse events associated with outpatient procedures performed by plastic surgeons should be mandated. Hemodynamic instability in the early postoperative period in an otherwise healthy patient may be due to fluid overload, lidocaine toxicity, or to hemorrhagic shock and must be recognized and treated aggressively. Guidelines for the safe practice of large-volume liposuction need to be established. 相似文献
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High-level expression of the Endo-beta-N-acetylglucosaminidase F2 gene in E.coli: one step purification to homogeneity 总被引:1,自引:0,他引:1
The Endo F2gene was overexpressed in E.coli as a fusion protein joined to
the maltose-binding protein. MBP-Endo F2was found in a highly enriched
state as insoluble, inactive inclusion bodies. Extraction of the inclusion
bodies with 20% acetic acid followed by exhaustive dialysis rendered the
fusion protein active and soluble. MBP-Endo F2was digested with Factor
Xaand purified on Q-Sepharose. The enzyme was homogeneous by SDS-PAGE, and
appeared as a single symmetrical peak on HPLC. Analysis of the
amino-terminus demonstrated conclusively that recombinant Endo F2was
homogeneous and identical to the native enzyme.
相似文献
115.
G.M.C. Renshaw G. Wise P.R. Dodd 《Comparative biochemistry and physiology. Part A, Molecular & integrative physiology》2010,155(4):486-492
Interactions between coral reef topography, tide cycles, and photoperiod provided selection pressure for adaptive physiological changes in sheltered hypoxic niches to be exploited by specialized tropical reef fish. The epaulette shark Hemiscyllium ocellatum withstands cyclic hypoxia in its natural environment, many hours of experimental hypoxia, and anoxia for up to 5 h. It shows neuronal hypometabolism in response to 5% oxygen saturation. Northern-hemisphere hypoxia- and anoxia-tolerant vertebrates that over-winter under ice alter their inhibitory to excitatory neurotransmitter balance to forestall brain ATP depletion in the absence of oxidative phosphorylation. GABA immunochemistry, HPLC analysis and receptor binding studies in H. ocellatum cerebellum revealed a heterogeneous regional accumulation of neuronal GABA despite no change in its overall concentration, and a significant increase in GABAA receptor density without altered binding affinity. Increased GABAA receptor density would protect the cerebellum during reoxygenation when transmitter release resumes. While all hypoxia- and anoxia-tolerant teleosts examined to date respond to low oxygen levels by elevating brain GABA, the phylogenetically older epaulette shark did not, suggesting that it uses an alternative neuroprotective mechanism for energy conservation. This may reflect an inherent phylogenetic difference, or represent a novel ecophysiological adaptation to cyclic variations in the availability of oxygen. 相似文献
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Ru Zhang Robert R. Wise Kimberly R. Struck Thomas D. Sharkey 《Photosynthesis research》2010,105(2):123-134
Photosynthesis is inhibited by heat stress. This inhibition is rapidly reversible when heat stress is moderate but irreversible
at higher temperature. Absorbance changes can be used to detect a variety of biophysical parameters in intact leaves. We found
that moderate heat stress caused a large reduction of the apparent absorbance of green light in light-adapted, intact Arabidopsis thaliana leaves. Three mechanisms that can affect green light absorbance of leaves, namely, zeaxanthin accumulation (absorbance peak
at 505 nm), the electrochromic shift (ECS) of carotenoid absorption spectra (peak at 518 nm), and light scattering (peak at
535 nm) were investigated. The change of green light absorbance caused by heat treatment was not caused by changes of zeaxanthin
content nor by the ECS. The formation of non-photochemical quenching (NPQ), chloroplast movements, and chloroplast swelling
and shrinkage can all affect light scattering inside leaves. The formation of NPQ under high temperature was not well correlated
with the heat-induced absorbance change, and light microscopy revealed no appreciable changes of chloroplast location because
of heat treatment. Transmission electron microscopy results showed swollen chloroplasts and increased number of plastoglobules
in heat-treated leaves, indicating that the structural changes of chloroplasts and thylakoids are significant results of moderate
heat stress and may explain the reduced apparent absorbance of green light under moderately high temperature. 相似文献
118.
Normal red blood cells and irreversibly sickled red cells (ISCs) were incubated in Concanavalin A (Con A) prior to freeze-etching. The Con A molecules could be visualized on the cells without conjugating the Con A to a larger molecule. No differences in the number or distribution of the Con A binding sites were observed between normal red cells and ISCs. Moreover, the number and distribution of the intramembranous particles (IMP) present on the fracture face of the cytoplasmic membrane leaflet were the same in normal cells and ISCs. Thus, the membrane differences that do exist between normal red cells and ISCs in terms of cell shape and adhesiveness are not reflected in Con A binding or IMP number and distribution. 相似文献
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120.
Wise MJ 《The New phytologist》2007,175(4):773-784
As part of a study of the ecological and evolutionary dynamics of herbivore resistance in Solanum carolinense (horsenettle), potential genetic constraints to the evolution of resistance to 11 of its most common herbivores were investigated. Leaf, flower, fruit, and stem herbivory were measured in a field experiment involving 24 ramets of each of 40 horsenettle genets. The experimental plant population contained significant genetic variation for resistance to all 11 species of herbivore. For only one species was there an indication of a genotype-by-environment interaction in the expression of resistance that might constrain its evolution. Genetic correlations in resistance to different species were common but not universal, with seven negative and 12 positive correlations out of the 55 pairwise species comparisons. Correlations were independent of plant part fed upon. The evolution of the resistance of horsenettle to most of its diverse community of herbivores does not appear to be prevented by a lack of genetic variation or by genotype-by-environment interactions in resistance. Negative genetic correlations in resistance to different herbivores may play a small role in slowing the evolution of resistance, but positive correlations may play at least as large a role in facilitating its evolution. 相似文献