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201.
The structure of the enzyme p-hydroxybenzoate hydroxylase (EC 1.14.13.2) in a complex with its substrate has been determined at a resolution of 2.5 Å. The molecular weight is 43,000 and the dimensions of one molecule are approximately 70 Å × 50 Å × 45 Å. The crystal structure contains dimers of these molecules. Approximately 16% of the residues occur in β-sheets and 26% in α-heliees. The molecule can be divided into three domains. The active site, near the isoalloxazine ring, is formed by side-chains of the three domains. The N-5 edge of the isoalloxazine ring points to p-hydroxybenzoate, which is bound in a deep cleft. 相似文献
202.
Kinetic properties of triose-phosphate isomerase from Trypanosoma brucei brucei. A comparison with the rabbit muscle and yeast enzymes 总被引:6,自引:0,他引:6
The kinetic properties of Trypanosoma brucei brucei triose-phosphate isomerase are compared with those of the commercially available rabbit muscle and yeast enzymes and with published data on the chicken muscle enzyme. With glyceraldehyde 3-phosphate as substrate Km = 0.25 +/- 0.05 mM and kcat = 3.7 X 10(5) min-1. With dihydroxyacetone phosphate as substrate Km = 1.2 +/- 0.1 mM and kcat = 6.5 X 10(4) min-1. The pH dependence of Km and Vmax at 0.1 M ionic strength is in agreement with the results published for the yeast and chicken muscle enzymes. At ionic strength below 0.05 M the effect of a charged group specific for the trypanosomal enzyme and absent from the yeast and rabbit muscle enzymes becomes detectable. This effect significantly increases Km whereas Vmax becomes slightly higher. Trypanosomal triose-phosphate isomerase is inhibited by sulphate, phosphate and arsenate ions, by 2-phosphoglycolate and a number of documented inhibitors in the same concentration range as are the other triose-phosphate isomerases. The trypanocidal drug, Suramin inhibits T. brucei and rabbit muscle triose-phosphate isomerase to the same extent while leaving the yeast enzyme relatively unaffected. 相似文献
203.
Feline W Lindhout Sybren Portegies Robbelien Kooistra Lotte J Herstel Riccardo Stucchi Jessica J A Hummel Nicky Scheefhals Eugene A Katrukha Maarten Altelaar Harold D MacGillavry Corette J Wierenga Casper C Hoogenraad 《The EMBO journal》2021,40(10)
Axon formation critically relies on local microtubule remodeling and marks the first step in establishing neuronal polarity. However, the function of the microtubule‐organizing centrosomes during the onset of axon formation is still under debate. Here, we demonstrate that centrosomes play an essential role in controlling axon formation in human‐induced pluripotent stem cell (iPSC)‐derived neurons. Depleting centrioles, the core components of centrosomes, in unpolarized human neuronal stem cells results in various axon developmental defects at later stages, including immature action potential firing, mislocalization of axonal microtubule‐associated Trim46 proteins, suppressed expression of growth cone proteins, and affected growth cone morphologies. Live‐cell imaging of microtubules reveals that centriole loss impairs axonal microtubule reorganization toward the unique parallel plus‐end out microtubule bundles during early development. We propose that centrosomes mediate microtubule remodeling during early axon development in human iPSC‐derived neurons, thereby laying the foundation for further axon development and function. 相似文献