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991.
B. Wen A. Ström A. Tasker G. West G. A. Tucker 《Plant biology (Stuttgart, Germany)》2013,15(6):1025-1032
Post‐harvest storage is largely limited by fruit softening, a result of cell wall degradation. Pectin methylesterase (PE) (EC 3.1.1.11) is a major hydrolase responsible for pectin de‐esterification in the cell wall, a response to fruit ripening. Two major PE isoforms, PE1 and PE2, have been isolated from tomato (Solanum lycopersicon) pericarp tissue and both have previously been down‐regulated using antisense suppression. In this paper, PE1 and PE2 double antisense tomato plants were successfully generated through crossing the two single antisense lines. In the double antisense fruit, approximately 10% of normal PE activity remained and ripening associated pectin de‐esterification was almost completely blocked. However, double antisense fruit softened normally during ripening. In tomato fruit, the PE1 isoform was found to contribute little to total PE activity and have little effect on the degree of esterification of pectin. In contrast, the other dominant fruit isoform, PE2, has a major impact on de‐esterification of total pectin. PE2 appears to act on non‐CDTA‐soluble pectin during ripening and on CDTA‐soluble pectin before the start of ripening in a potentially block‐wise fashion. 相似文献
992.
993.
Our understanding of how natural selection should shape sex allocation is perhaps more developed than for any other trait. However, this understanding is not matched by our knowledge of the genetic basis of sex allocation. Here, we examine the genetic basis of sex ratio variation in the parasitoid wasp Nasonia vitripennis, a species well known for its response to local mate competition (LMC). We identified a quantitative trait locus (QTL) for sex ratio on chromosome 2 and three weaker QTL on chromosomes 3 and 5. We tested predictions that genes associated with sex ratio should be pleiotropic for other traits by seeing if sex ratio QTL co-occurred with clutch size QTL. We found one clutch size QTL on chromosome 1, and six weaker QTL across chromosomes 2, 3 and 5, with some overlap to regions associated with sex ratio. The results suggest rather limited scope for pleiotropy between these traits. 相似文献
994.
Effect of postactivation potentiation on swimming starts in international sprint swimmers 总被引:1,自引:0,他引:1
Kilduff LP Cunningham DJ Owen NJ West DJ Bracken RM Cook CJ 《Journal of strength and conditioning research / National Strength & Conditioning Association》2011,25(9):2418-2423
The aim of this study was to investigate the effects of postactivation potentiation (PAP) on swim start performance (time to 15 m) in a group of international sprint swimmers. Nine international sprint swimmers (7 men and 2 women) volunteered and gave informed consent for this study, which was approved by the university ethics committee. Initially, swimmers performed a countermovement jump (CMJ) on a portable force platform (FP) at baseline and at the following time points ~15 seconds, 4, 8, 12, and 16 minutes after a PAP stimulus (1 set of 3 repetitions at 87% 1 repetition maximum [RM]) to individually determine the recovery time required to observe enhanced muscle performance. On 2 additional days, swimmers performed a swim start to 15 m under 50-m freestyle race conditions, which was preceded by either their individualized race specific warm-up or a PAP stimulus (1 set of 3 repetitions at 87% 1RM). Both trials were recorded on 2 cameras operating at 50 Hz with camera 1 located at the start and camera 2 at the 15-m mark. Peak vertical force (PVF) and peak horizontal force (PHF) were measured during all swim starts from a portable FP placed on top of the swim block. A repeated measures analysis of variance revealed a significant time effect with regard to power output (PO) (F = 20.963, p < 0.01) and jump height (JH) (F = 14.634, p < 0.01) with a paired comparison indicating a significant increase in PO and JH after 8 minutes of recovery from the PAP stimulus. There was a significant increase in both PHF and PVF after the PAP stimulus compared to the swim-specific warm-up during the swim start (PHF 770 ± 228 vs. 814 ± 263 N, p = 0.018; PVF: 1,462 ± 280 vs. 1,518 ± 311 N, p = 0.038); however, time to 15 m was the same when both starts were compared (7.1 ± 0.8 vs. 7.1 ± 0.8 seconds, p = 0.447). The results from this study indicate that muscle performance during a CMJ is enhanced after a PAP stimulus providing adequate recovery (~8 minutes) is given between the 2 activities. In addition, this study demonstrated that swimmers performed equally well in terms of time to 15 m when a PAP stimulus was compared to their individualized race specific warm-up and indicates that PAP may be a useful addition to a warm-up protocol before races. However, more research is required to fully understand the role PAP plays in swim performance. 相似文献
995.
Andrabi SA Kang HC Haince JF Lee YI Zhang J Chi Z West AB Koehler RC Poirier GG Dawson TM Dawson VL 《Nature medicine》2011,17(6):692-699
Glutamate acting on N-methyl-D-aspartate (NMDA) receptors induces neuronal injury following stroke, through activation of poly(ADP-ribose) polymerase-1 (PARP-1) and generation of the death molecule poly(ADP-ribose) (PAR) polymer. Here we identify Iduna, a previously undescribed NMDA receptor-induced survival protein that is neuroprotective against glutamate NMDA receptor-mediated excitotoxicity both in vitro and in vivo and against stroke through interfering with PAR polymer-induced cell death (parthanatos). Iduna's protective effects are independent and downstream of PARP-1 activity. Iduna is a PAR polymer-binding protein, and mutation at the PAR polymer binding site abolishes the PAR binding activity of Iduna and attenuates its protective actions. Iduna is protective in vivo against NMDA-induced excitotoxicity and middle cerebral artery occlusion-induced stroke in mice. To our knowledge, these results define Iduna as the first known endogenous inhibitor of parthanatos. Interfering with PAR polymer signaling could be a new therapeutic strategy for the treatment of neurologic disorders. 相似文献
996.
Susan Majka Moira Hagen Thomas Blackwell Julie Harral Jennifer A Johnson Robert Gendron Helene Paradis Daniel Crona James E Loyd Eva Nozik-Grayck Kurt R Stenmark James West 《Respiratory research》2011,12(1):84
Background
Pulmonary arterial hypertension (PAH) is thought to be driven by dysfunction of pulmonary vascular microendothelial cells (PMVEC). Most hereditary PAH is associated with BMPR2 mutations. However, the physiologic and molecular consequences of expression of BMPR2 mutations in PMVEC are unknown.Methods
In vivo experiments were performed on adult mice with conditional endothelial-specific expression of the truncation mutation Bmpr2delx4+, with age-matched transactivator-only mice as controls. Phenotype was assessed by RVSP, counts of muscularized vessels and proliferating cells, and staining for thromboses, inflammatory cells, and apoptotic cells. The effects of BMPR2 knockdown in PMVEC by siRNA on rates of apoptosis were assessed. Affymetrix expression arrays were performed on PMVEC isolated and cultured from triple transgenic mice carrying the immortomouse gene, a transactivator, and either control, Bmpr2delx4+ or Bmpr2R899X mutation.Results
Transgenic mice showed increased RVSP and corresponding muscularization of small vessels, with histologic alterations including thrombosis, increased inflammatory cells, increased proliferating cells, and a moderate increase in apoptotic cells. Expression arrays showed alterations in specific pathways consistent with the histologic changes. Bmpr2delx4+ and Bmpr2R899X mutations resulted in very similar alterations in proliferation, apoptosis, metabolism, and adhesion; Bmpr2delx4+ cells showed upregulation of platelet adhesion genes and cytokines not seen in Bmpr2R899X PMVEC. Bmpr2 mutation in PMVEC does not cause a loss of differentiation markers as was seen with Bmpr2 mutation in smooth muscle cells.Conclusions
Bmpr2 mutation in PMVEC in vivo may drive PAH through multiple, potentially independent, downstream mechanisms, including proliferation, apoptosis, inflammation, and thrombosis. 相似文献997.
Sucgang R Kuo A Tian X Salerno W Parikh A Feasley CL Dalin E Tu H Huang E Barry K Lindquist E Shapiro H Bruce D Schmutz J Salamov A Fey P Gaudet P Anjard C Babu MM Basu S Bushmanova Y van der Wel H Katoh-Kurasawa M Dinh C Coutinho PM Saito T Elias M Schaap P Kay RR Henrissat B Eichinger L Rivero F Putnam NH West CM Loomis WF Chisholm RL Shaulsky G Strassmann JE Queller DC Kuspa A Grigoriev IV 《Genome biology》2011,12(2):R20
Background
The social amoebae (Dictyostelia) are a diverse group of Amoebozoa that achieve multicellularity by aggregation and undergo morphogenesis into fruiting bodies with terminally differentiated spores and stalk cells. There are four groups of dictyostelids, with the most derived being a group that contains the model species Dictyostelium discoideum.Results
We have produced a draft genome sequence of another group dictyostelid, Dictyostelium purpureum, and compare it to the D. discoideum genome. The assembly (8.41 × coverage) comprises 799 scaffolds totaling 33.0 Mb, comparable to the D. discoideum genome size. Sequence comparisons suggest that these two dictyostelids shared a common ancestor approximately 400 million years ago. In spite of this divergence, most orthologs reside in small clusters of conserved synteny. Comparative analyses revealed a core set of orthologous genes that illuminate dictyostelid physiology, as well as differences in gene family content. Interesting patterns of gene conservation and divergence are also evident, suggesting function differences; some protein families, such as the histidine kinases, have undergone little functional change, whereas others, such as the polyketide synthases, have undergone extensive diversification. The abundant amino acid homopolymers encoded in both genomes are generally not found in homologous positions within proteins, so they are unlikely to derive from ancestral DNA triplet repeats. Genes involved in the social stage evolved more rapidly than others, consistent with either relaxed selection or accelerated evolution due to social conflict.Conclusions
The findings from this new genome sequence and comparative analysis shed light on the biology and evolution of the Dictyostelia.998.
The increasing importance and complexity of migration globally also implies a global increase in return migration, and thus an increased interest in the health of returning migrants. The health of returning migrants is impacted by the cumulative exposure to social determinants and risk factors of health during the migration process, during the return movement, and following return. Circular migration often occurs among the diaspora, which can result in the transfer of knowledge and skills that contribute to development, including health system strengthening. Migrants with dual nationality often return to countries with better health services than their country of origin when they are sick and can not get care at home. To maintain and improve the health of returning migrants, multi-sectoral policies at global and national levels should facilitate access to appropriate and equitable health services, social services, and continuity of care across and within borders. 相似文献
999.
1000.
Thomas D Pinkney David C Bartlett William Hawkins Tony Mak Haney Youssef Kaori Futaba Gareth Harrison Adrian Gheorghe Jennifer M Bradbury Melanie J Calvert George Dowswell Laura Magill Val Redman Sue Wilson David Leaper Dion G Morton the West Midlands Research Collaborative 《Trials》2011,12(1):217