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71.
THE INTERSPECIFIC ORIGIN OF B CHROMOSOMES: EXPERIMENTAL EVIDENCE 总被引:1,自引:0,他引:1
Francisco Perfectti John H. Werren 《Evolution; international journal of organic evolution》2001,55(5):1074-1074
Abstract.— A centric fragment was generated during the introgression of a chromosome region from Nasonia giraulti into N. vitripennis. This neo B chromosome carries the N. giraulti or 123+ gene for wild‐type eye color. Using this phenotypic effect, the transmission of this chromosome was analyzed. The supernumerary chromosome showed less than Mendelian segregation rate in meiosis and some mitotic instability manifested as mosaic phenotype for eye color. However, transmission rate and mitotic stability increased over successive generations. The transmission rate through male gametogenesis was nearly 100%. These results support the interspecific hybridization model for B chromosome origin and reveal that problems in chromosome stability can persist for several generations after “foreign chromosomes” are introduced into a different species. We suggest that hybrid zones should be investigated as possible sites for neo‐B chromosome generation. 相似文献
72.
73.
According to theory, F(2) hybrid breakdown (lethality or sterility) is due to incompatibilities between interacting genes of the different species (i.e., the breaking up of coadapted gene complexes). Detection of such incompatibilities is particularly straightforward in haplodiploid species, because virgin F(1) hybrid females will produce haploid recombinant F(2) males. This feature allows for screening of the complete genome for recessive genetic incompatibilities. Crosses were performed between Nasonia vitripennis (v) and its sibling species N. giraulti (g). First, a linkage map was produced using RAPD markers. RAPD markers showed an overall bias toward vitripennis alleles, a pattern not predicted by the basic two-interactor Dobzhansky-Muller model. Recovery patterns of visible markers were consistent with those of linked RAPD markers. If particular genetic interactions between two loci are causing hybrid lethality, then those genotypes should be underrepresented or absent among adult F(2) males. Four sets of significant incompatibilities were detected by performing pairwise comparisons of markers on different chromosomes. Likely explanations for the observed patterns are maternal effect-zygotic gene incompatibilities or clustering of incompatibility loci. Due to the short generation time, advantages of haplodiploidy, and availability of markers, Nasonia promises to be a productive system for investigating the genetics of hybrid inviability. 相似文献
74.
In haplodiploid species, Wolbachia-induced cytoplasmic incompatibility (CI) can be expressed in one of two ways: as a "conversion" of diploid fertilized eggs into haploid males or as embryonic mortality. Here we describe CI-type variation within the parasitic wasp genus Nasonia and genetically analyze the basis of this variation. We reach four main conclusions: (i) CI is expressed primarily as conversion in N. vitripennis, but as embryonic mortality in the sibling species N. giraulti and N. longicornis; (ii) the difference in CI type between N. giraulti (mortality) and N. vitripennis (conversion) is determined by host nuclear genotype rather than by Wolbachia differences; (iii) N. vitripennis "conversion genes" are recessive in hybrid females; and (iv) a difference in CI level between the sibling species N. giraulti and N. longicornis is due to the different Wolbachia infections in the species rather than to the host genotype. These results show that host nuclear genes can influence the type of CI present in a species. On the basis of these findings, we propose a model for how different CI types evolve in haplodiploids due to selection on nuclear genes modifying CI. 相似文献
75.
PSR (paternal sex ratio) chromosomes are a type of supernumerary (or B) chromosomes that occur in haplodiploid arthropods. They are transmitted through sperm but then cause loss of the paternal chromosomes (except themselves) early in development. As a result, PSR chromosomes convert diploid fertilized eggs (which would normally develop into females) into haploid males that carry a PSR chromosome. Because they act by completely eliminating the haploid genome of their hosts, PSR chromosomes are the most extreme form of selfish or parasitic DNA known. PSR was originally described in the parasitic wasp Nasonia vitripennis (Pteromalidae). A second PSR chromosome has been found in Trichogramma kaykai, an egg parasitoid from a different family of Hymenoptera (Trichogrammatidae). We argue that PSR chromosomes are likely to be widespread in haplodiploid organisms, but have so far gone under reported due to a paucity of population genetic studies in haplodiploids. We describe the two known PSR systems and related phenomena, and models indicating the conditions conducive to increase of PSR like chromosomes in haplodiploids. 相似文献
76.
There is growing evidence that sex determination in a wide range of organisms is determined by interactions between maternal-effect genes and zygotically expressing genes. Maternal-effect genes typically produce products (e.g., mRNA or proteins) that are placed into the egg during oogenesis and therefore depend upon maternal genotype. Here it is shown that maternal-effect and zygotic genes are subject to conflicting selective pressures over sex determination in species with partial inbreeding or subdivided populations. The optimal sex ratios for maternal-effect genes and zygotically expressing genes are derived for two models: partial inbreeding (sibmating) and subdivided populations with local mating in temporary demes (local mate competition). In both cases, maternal-effect genes are selected to bias sex determination more toward females than are zygotically expressed genes. By investigating the invasion criteria for zygotic genes in a population producing the maternal optimum (and vice versa), it is shown that genetic conflict occurs between these genes. Even relatively low levels of inbreeding or subdivision can result in maternal-zygotic gene conflict over sex determination. The generality of maternal-zygotic gene conflict to sex determination evolution is discussed; such conflict should be considered in genetic studies of sex-determining mechanisms. 相似文献
77.
Revisiting <Emphasis Type="Italic">Wolbachia</Emphasis> Supergroup Typing Based on WSP: Spurious Lineages and Discordance with MLST 总被引:2,自引:0,他引:2
The obligate intracellular bacteria Wolbachia are taxonomically subdivided into eight supergroups (named A-H). Supergroup typing of strains has been mostly based on phylogenetic inference of the Wolbachia surface protein (wsp), a gene that recently has been shown to experience high rates of recombination. This brings into question its suitability not only for microtaxonomy, but also for supergroup classification of the genus. A Multilocus Sequence Typing (MLST) scheme for Wolbachia has recently been developed that types strains based on five conserved genes, thus providing a rigorous supergroup annotation of strains. Here we report striking discrepancies in supergroup designation between MLST and wsp inferences, and propose a revision of current methods for Wolbachia supergroup typing. Transfer of whole wsp gene sequences between supergroups A and B has occurred. Furthermore, as a result of intragenic recombination, wsp phylogeny creates spurious basal lineages that are not supported by MLST. For example, the proposed supergroup G, based upon wsp alone, likely represents only a wsp recombinant clade. Removal of supergroup G is advised until and unless the existence of this lineage is substantiated by other sequence information (e.g., MLST). We recommend a full characterization MLST for a correct strain typing, while, based on the current data set, use of a single MLST gene can be effective for supergroup designation of A and B strains. Finally, we note that the sharing of wsp sequences between A and B strains indicates a strong genetic cohesiveness of Wolbachia strains, supporting designation of these bacteria within the same species, W. pipientis. 相似文献
78.
<Emphasis Type="Italic">Wolbachia</Emphasis> Are Present in Southern African Scorpions and Cluster with Supergroup F 总被引:1,自引:0,他引:1
The presence and distribution of the intracellular bacteria Wolbachia in the arthropod subphylum Chelicerata (including class Arachnida) has not been extensively explored. Here we report the discovery of Wolbachia in scorpions. Five strains found in host species of the genus Opistophthalmus (Southern African burrowing scorpions) have been characterized by Multilocus Sequence Typing and by Wolbachia Surface Protein. Phylogenetic analyses indicate clustering in the supergroup F and a high genetic relatedness among all scorpion strains as a result of a potential transmission within the host genus. The F-group is an uncommon lineage compared to the A and B supergroups, although it is present in a broad range of hosts (including insects, filarial nematodes, and now arachnids) and across a large geographical area (e.g., North America, Africa, Europe, and Australia). It also shows no evidence of recombination and has a significantly higher genetic diversity than supergroup A and B. Overall, this pattern suggests an older radiation of F-strains with respect to A and B-strains, followed by limited horizontal transmission across host genera and reduced genetic flux among strains. A more extensive sampling of supergroup F-strains is required to confirm this scenario. 相似文献
79.
It is commonly assumed that creatine kinase (CK) activity in plasma is related to the state of an inflammatory response at 24-48 h, and also it has shown biphasic patterns after a marathon run. No information is available on CK isoenzymes after an ultra-marathon run. The purpose of the present study is to examine the CK isoenzymes after a 200 km ultra-marathon run and during the subsequent recovery. Blood samples were obtained during registration 1 2 h before the 200-km race and during the race at 100 km, 150 km and at the end of 200 km, as well as after a 24 h period of recovery. Thirty-two male ultra-distance runners participated in the study. Serum CPK showed a marked increase throughout the race and 24 h recovery period (p < 0.001). Serum CK during the race occurs mostly in the CK-MM isoform and only minutely in the CK-MB isoform and is unchanged in the CK-BB isoform. High-sensitivity C-reactive protein (hs-CRP), oestradiol, AST and ALT increased significantly from the pre-race value at 100 km and a further increase took place by the end of the 200 km run. The results of our study demonstrate a different release pattern of creatine kinase after an ultra-distance (200 km) run compared to the studies of marathon running and intense eccentric exercise, and changes in several biomarkers, indicative of muscle damage during the race, were much more pronounced during the latter half (100–200 km) of the race. However, the increases in plasma concentration of muscle enzymes may reflect not only structural damage, but also their rate of clearance. 相似文献
80.