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991.
992.
Synthesis in vitro of vesicular stomatitis virus proteins in cytoplasmic extracts of L cells 总被引:8,自引:0,他引:8
H P Ghosh F Toneguzzo S Wells 《Biochemical and biophysical research communications》1973,54(1):228-233
All five vesicular stomatitis virus (VSV) proteins, namely, L, G,N,NS and M are synthesized by a post-nuclear extract from cultured L cells infected with VSV. When, however, membrane bound polysomes are removed from the cytoplasmic extract only four virus specific proteins (L,N,NS and M) were synthesized. 相似文献
993.
994.
Helically organized macroaggregates of pigment-protein complexes in chloroplasts: evidence from circular intensity differential scattering 总被引:4,自引:0,他引:4
Angle dependence of circular intensity differential scattering (CIDS) and of nonpolarized scattering was determined in isolated spinach chloroplasts at 514.5 nm. CIDS between 0 degrees and 170 degrees was independent of the nonpolarized scattering and showed intense lobes of alternating signs, exhibiting the negative and positive maxima around 15 degrees and 70 degrees, respectively. These results provide experimental evidence for the existence of large helically organized macroaggregates of pigment-protein complexes in thylakoid membranes. Modeling of the CIDS data by a simple helical array of uniaxial polarizable groups suggests that the chiral structure is left-handed with pitch and radius of the order of 385 nm. 相似文献
995.
996.
Rapid Fluorescein and Protein Assay Method for Fluorescent-Antibody Conjugates 总被引:23,自引:5,他引:18 下载免费PDF全文
A method is presented for the rapid determination of the fluorescein content, protein content, and fluorescein-to-protein ratio for immune globulin conjugates with fluorescein isothiocyanate as the fluor. This method is based on the absorbance of the fluorescent antibody at those wavelengths primarily associated with the fluorescein and gamma-globulin fractions, and permits these materials to be determined by a single nondestructive analytical procedure. A small sample of the fluorescent antibody, in many cases 0.1 ml or less, is adequate for the above determinations. A nomograph is presented which allows simultaneous determination of the materials from the observed absorbance at the two wavelengths. The method is sufficiently accurate for most applications of the fluorescent-antibody techniques. Although this procedure has been developed primarily for fluorescent-antibody conjugates prepared from rabbit gamma-globulin, it can be used directly for antibodies prepared from other animals provided the gamma-globulin is relatively free from albumin. 相似文献
997.
998.
Travis K. Warren Chris A. Whitehouse Jay Wells Lisa Welch Jay S. Charleston Alison Heald Donald K. Nichols Marc E. Mattix Gustavo Palacios Jeffrey R. Kugleman Patrick L. Iversen Sina Bavari 《PLoS neglected tropical diseases》2016,10(2)
Marburg virus (MARV) is an Ebola-like virus in the family Filovirdae that causes sporadic outbreaks of severe hemorrhagic fever with a case fatality rate as high as 90%. AVI-7288, a positively charged antisense phosphorodiamidate morpholino oligomer (PMOplus) targeting the viral nucleoprotein gene, was evaluated as a potential therapeutic intervention for MARV infection following delayed treatment of 1, 24, 48, and 96 h post-infection (PI) in a nonhuman primate lethal challenge model. A total of 30 cynomolgus macaques were divided into 5 groups of 6 and infected with 1,830 plaque forming units of MARV subcutaneously. AVI-7288 was administered by bolus infusion daily for 14 days at 15 mg/kg body weight. Survival was the primary endpoint of the study. While none (0 of 6) of the saline group survived, 83–100% of infected monkeys survived when treatment was initiated 1, 24, 48, or 96 h post-infection (PI). The antisense treatment also reduced serum viremia and inflammatory cytokines in all treatment groups compared to vehicle controls. The antibody immune response to virus was preserved and tissue viral antigen was cleared in AVI-7288 treated animals. These data show that AVI-7288 protects NHPs against an otherwise lethal MARV infection when treatment is initiated up to 96 h PI. 相似文献
999.
Reassessing Ethnic Differences in Mean BMI and Changes Between 2007 and 2013 in English Children 下载免费PDF全文