High-fiber diet mitigates maternal obesity-induced cognitive and social dysfunction in the offspring via gut-brain axis

1. Download : Download high-res image (180KB)
2. Download : Download full-size image

     

STING promotes senescence,apoptosis, and extracellular matrix degradation in osteoarthritis via the NF-κB signaling pathway

Damaged deoxyribonucleic acid (DNA) is a primary pathologic factor for osteoarthritis (OA); however, the mechanism by which DNA damage drives OA is unclear. Previous research demonstrated that the cyclic GMP-AMP synthase (cGAS)-stimulator of interferon genes (STING) participates in DNA damage response. As a result, the current study aimed at exploring the role STING, which is the major effector in the cGAS-STING signaling casacde, in OA progress in vitro, as well as in vivo. In this study, the expression of STING was evaluated in the human and mouse OA tissues, and in chondrocytes exposed to interleukin-1 beta (IL-1β). The influences of STING on the metabolism of the extracellular matrix (ECM), apoptosis, and senescence, were assessed in STING overexpressing and knocking-down chondrocytes. Moreover, the NF-κB-signaling casacde and its role in the regulatory effects of STING on ECM metabolism, apoptosis, and senescence were explored. The STING knockdown lentivirus was intra-articularly injected to evaluate its therapeutic impact on OA in mice in vivo. The results showed that the expression of STING was remarkably elevated in the human and mouse OA tissues and in chondrocytes exposed to IL-1β. Overexpression of STING promoted the expression of MMP13, as well as ADAMTS5, but suppressed the expression of Aggrecan, as well as Collagen II; it also enhanced apoptosis and senescence in chondrocytes exposed to and those untreated with IL-1β. The mechanistic study showed that STING activated NF-κB signaling cascade, whereas the blockage of NF-κB signaling attenuated STING-induced apoptosis and senescence, and ameliorated STING-induced ECM metabolism imbalance. In in vivo study, it was demonstrated that STING knockdown alleviated destabilization of the medial meniscus-induced OA development in mice. In conclusion, STING promotes OA by activating the NF-κB signaling cascade, whereas suppression of STING may provide a novel approach for OA therapy.Subject terms: Apoptosis, Senescence     

An epithelial–mesenchymal transition-related long noncoding RNA signature correlates with the prognosis and progression in patients with bladder cancer

Bladder cancer is a common malignant tumour worldwide. Epithelial–mesenchymal transition (EMT)-related biomarkers can be used for early diagnosis and prognosis of cancer patients. To explore, accurate prediction models are essential to the diagnosis and treatment for bladder cancer. In the present study, an EMT-related long noncoding RNA (lncRNA) model was developed to predict the prognosis of patients with bladder cancer. Firstly, the EMT-related lncRNAs were identified by Pearson correlation analysis, and a prognostic EMT-related lncRNA signature was constructed through univariate and multivariate Cox regression analyses. Then, the diagnostic efficacy and the clinically predictive capacity of the signature were assessed. Finally, Gene set enrichment analysis (GSEA) and functional enrichment analysis were carried out with bioinformatics. An EMT-related lncRNA signature consisting of TTC28-AS1, LINC02446, {"type":"entrez-nucleotide","attrs":{"text":"AL662844.4","term_id":"17978158","term_text":"AL662844.4"}}AL662844.4, {"type":"entrez-nucleotide","attrs":{"text":"AC105942.1","term_id":"18129419","term_text":"AC105942.1"}}AC105942.1, {"type":"entrez-nucleotide","attrs":{"text":"AL049840.3","term_id":"7406443","term_text":"AL049840.3"}}AL049840.3, SNHG26, USP30-AS1, PSMB8-AS1, {"type":"entrez-nucleotide","attrs":{"text":"AL031775.1","term_id":"4071041","term_text":"AL031775.1"}}AL031775.1, {"type":"entrez-nucleotide","attrs":{"text":"AC073534.1","term_id":"8655969","term_text":"AC073534.1"}}AC073534.1, {"type":"entrez-nucleotide","attrs":{"text":"U62317.2","term_id":"6862558","term_text":"U62317.2"}}U62317.2, C5orf56, {"type":"entrez-nucleotide","attrs":{"text":"AJ271736.1","term_id":"8979791","term_text":"AJ271736.1"}}AJ271736.1, and {"type":"entrez-nucleotide","attrs":{"text":"AL139385.1","term_id":"6996206","term_text":"AL139385.1"}}AL139385.1 was constructed. The diagnostic efficacy of the signature was evaluated by the time-dependent receiver-operating characteristic (ROC) curves, in which all the values of the area under the ROC (AUC) were more than 0.73. A nomogram established by integrating clinical variables and the risk score confirmed that the signature had a good clinically predict capacity. GSEA analysis revealed that some cancer-related and EMT-related pathways were enriched in high-risk groups, while immune-related pathways were enriched in low-risk groups. Functional enrichment analysis showed that EMT was associated with abundant GO terms or signaling pathways. In short, our research showed that the 14 EMT-related lncRNA signature may predict the prognosis and progression of patients with bladder cancer.     

微藻趋向运动及其靶向运输应用研究进展

微藻主要是指一类能进行光合自养的微生物,且很多藻株还兼具运动特性.因而将微藻与微流控芯片结合,实现精确靶向,或用于分子药物递送,在生物医学治疗和药效学分析等领域有重要的潜在应用价值,也是当今的研究热点之一.然而,目前关于微藻趋向运动的研究及潜在的应用的综述报道却相对较少.本文主要以模式微藻莱茵衣藻为例,概述基于微藻细胞...