Chlorophyll turnover in etiolated greening barley transferred to darkness: Isotopic (1-14C glutamic acid) evidence of dark chlorophyll synthesis in the absence of chlorophyll accumulation

Synthesis of chlorophyll was initiated in 5- to 6-day-old dark-grown barley (Hordeum vulgare L. cv. Clipper)seedlings by exposing them to light in the presence of 1-¹⁴ C glutamic acid supplied via the roots.The plants were then returned to darkness. At the end of light treatment (_T) and after 7 or 18 h dark treatment chlorophylls a and b were extracted, quantified (μgleaf¹). purified by HPLC to their magnesium-free derivatives (pheophytin a and b) and their molar radioactivities determined. After 2 h exposure to light followed by 6 h illumination in the presence of 1-¹⁴ C glutamic acid, seedlings had accumulated 4-7 nmol chlorophyll leaf¹ and had incorporated between 900-1 350 Bq (g fresh weight)¹ of radioactive label into the chlorophyll pool. When seedlings were transferred to darkness, label continued to be incorporated and after 18 h the radioactivity of the chlorophyll pool had increased by 300-700 Bq (g fresh weight)¹. Net chlorophyll content, however, remained constant during dark treatment. The increase in radioactivity of the chlorophyll pool in darkness represented the difference between a net increase of label incorporated into chlorophyll a and a small loss of label from chlorophyll b. The absence of measurable radioactivity in the phytol moiety of labelled chlorophyll a, extracted at the endof dark treatment, demonstrated thatincorporation of label was into the tetrapyrrole moiely of chlorophyll and not into the phytol chain. Light-independent incorporation of 1-¹⁴ C glutamic acid into chlorophyll of greening barley seedlings transferred to darkness indicates that chlorophyll synthesis continues when light is withheld. We interpret the net gain in radioactivity of chlorophyll in darkness, in the absence of a net gain in chlorophyll content, to chlorophyll turnover i.e. to simultaneous synthesis and breakdown of chlorophyll when etiolated greening barley seedlings are transferred to darkness.     

Herpetomonas megaseliae, Crithidia fasciculata, and Leptomonas collosoma (Kinetoplastida, Trypanosomatidae) in Feces of Lizards Fed Culture Forms of the Flagellates

ABSTRACT. Herpetomonas megaseliae, Crithidia fasciculata , and Leptomonas collosoma from culture survived gut passage in Anolis carolinensis following their ingestion by this lizard. Maximum persistence of H. megaseliae in lizards, as detected by fecal culture, was seven days. No invasion of tissues by H. megaseliae could be detected by means of sectioned material, stained impression slides, or cultures inoculated with material from organs. Crithidia fasciculata was evident in cloacal fluid for up to three days in wet mount preparations. Leptomonas collosoma was observed in feces 24 h after the organisms were fed to lizards. Both C. fasciculata and L. collosoma were cultured from feces of lizards fed the parasites 24 h earlier. Herpetomonas megaseliae was differentiated in lizard feces, with greater than 40% of the forms observed being paramastigotes or opisthomastigotes. Truncate, semispherical forms resembling choanomastigotes were seen, but the kinetoplast was posterior to the nucleus in some of these. Many forms showed extensive coiling of the axoneme within the body of the flagellate. Choanomastigotes and spheromastigotes of C. fasciculata and promastigotes, sphero-mastigotes and amastigotes of L. collosoma were also observed in the feces.     

Studies onGomphocarpus physocarpus: Further evidence of preferential feeding by the aphid,Aphis nerii on the internal phloem

Protoplasma - Penetration of the stems and leaves ofGomphocarpus physocarpus (E.Mey) by the aphid,Aphis nerii (B. de F.) was studied with light and phase microscopes. Penetration of the epidermis...     

Light penetration and the interrelationships between optical parameters in a turbid subtropical impoundment

An investigation of water transparency characteristics and light attenuation by waters of a turbid subtropical impoundment showed that allochthonous inputs of silt during summer floods, impoundment morphometry, and the warm monomictic thermal cycle were the main factors regulating the temporal and spatial variations in water transparency. Statistically significant relationships between the Secchi disc transparency, turbidity of the surface water, mean diffuse attenuation coefficient and beam attenuation coefficient, were established. These relationships allowed for an approximation of the 1 per cent of surface light intensity depth to be made by using any of the four parameters. The attenuation of blue light was greater than that of red light, owing to the effects of suspended clay particles on the spectral attenuation of light.     

Incubation studies on mineralization of organic sulphur and organic nitrogen

Summary Incubation studies were carried out to investigate the release of sulphur and nitrogen in West Indian soils. Sulphur and nitrogen released or fixed were estimated at 10 days intervals up to 60 days incubation period.All the soils released sulphate when incubated at 30°C. A rapid initial flush of mineralization of both sulphur and nitrogen took place in Cocal fine sand and Montreal sandy loam. In Piarco sandy clay loam and Mayaro sandy loam sulphur mineralization was not accompanied by a concomitant mineralization of nitrogen. An inconsistent pattern of release of sulphur and nitrogen was noticed in Montserrat clay, Akers sandy clay loam, Bellevue sandy clay loam and Soufriere cindery gravelly loamy sand.The release of sulphur does not appear to be related to the total amount of carbon, nitrogen or sulphur. Nitrogen mineralized was significantly correlated with total nitrogen and total sulphur. The correlation between organic matter and nitrogen mineralized was highly significant (r=0.87^**) whereas with sulphur mineralized it did not reach significance. This suggests that nitrogen and sulphur are not mineralized at the same rate in these soils.     

Linking microarray reporters with protein functions

Background  

The analysis of microarray experiments requires accurate and up-to-date functional annotation of the microarray reporters to optimize the interpretation of the biological processes involved. Pathway visualization tools are used to connect gene expression data with existing biological pathways by using specific database identifiers that link reporters with elements in the pathways.     

Fast Simulation of Mechanical Heterogeneity in the Electrically Asynchronous Heart Using the MultiPatch Module

Cardiac electrical asynchrony occurs as a result of cardiac pacing or conduction disorders such as left bundle-branch block (LBBB). Electrically asynchronous activation causes myocardial contraction heterogeneity that can be detrimental for cardiac function. Computational models provide a tool for understanding pathological consequences of dyssynchronous contraction. Simulations of mechanical dyssynchrony within the heart are typically performed using the finite element method, whose computational intensity may present an obstacle to clinical deployment of patient-specific models. We present an alternative based on the CircAdapt lumped-parameter model of the heart and circulatory system, called the MultiPatch module. Cardiac walls are subdivided into an arbitrary number of patches of homogeneous tissue. Tissue properties and activation time can differ between patches. All patches within a wall share a common wall tension and curvature. Consequently, spatial location within the wall is not required to calculate deformation in a patch. We test the hypothesis that activation time is more important than tissue location for determining mechanical deformation in asynchronous hearts. We perform simulations representing an experimental study of myocardial deformation induced by ventricular pacing, and a patient with LBBB and heart failure using endocardial recordings of electrical activation, wall volumes, and end-diastolic volumes. Direct comparison between simulated and experimental strain patterns shows both qualitative and quantitative agreement between model fibre strain and experimental circumferential strain in terms of shortening and rebound stretch during ejection. Local myofibre strain in the patient simulation shows qualitative agreement with circumferential strain patterns observed in the patient using tagged MRI. We conclude that the MultiPatch module produces realistic regional deformation patterns in the asynchronous heart and that activation time is more important than tissue location within a wall for determining myocardial deformation. The CircAdapt model is therefore capable of fast and realistic simulations of dyssynchronous myocardial deformation embedded within the closed-loop cardiovascular system.