全文获取类型
收费全文 | 292541篇 |
免费 | 35973篇 |
国内免费 | 199篇 |
专业分类
328713篇 |
出版年
2016年 | 2879篇 |
2015年 | 4321篇 |
2014年 | 5017篇 |
2013年 | 7083篇 |
2012年 | 8017篇 |
2011年 | 7925篇 |
2010年 | 5432篇 |
2009年 | 5120篇 |
2008年 | 7195篇 |
2007年 | 7386篇 |
2006年 | 6980篇 |
2005年 | 6934篇 |
2004年 | 6760篇 |
2003年 | 6858篇 |
2002年 | 6474篇 |
2001年 | 11094篇 |
2000年 | 11210篇 |
1999年 | 9229篇 |
1998年 | 3573篇 |
1997年 | 3751篇 |
1996年 | 3737篇 |
1995年 | 3467篇 |
1994年 | 3466篇 |
1993年 | 3437篇 |
1992年 | 8190篇 |
1991年 | 7970篇 |
1990年 | 7766篇 |
1989年 | 7686篇 |
1988年 | 7255篇 |
1987年 | 7208篇 |
1986年 | 6672篇 |
1985年 | 6822篇 |
1984年 | 5721篇 |
1983年 | 5136篇 |
1982年 | 4101篇 |
1981年 | 3945篇 |
1980年 | 3575篇 |
1979年 | 5926篇 |
1978年 | 4637篇 |
1977年 | 4442篇 |
1976年 | 4237篇 |
1975年 | 4580篇 |
1974年 | 5041篇 |
1973年 | 4931篇 |
1972年 | 4591篇 |
1971年 | 4138篇 |
1970年 | 3646篇 |
1969年 | 3669篇 |
1968年 | 3272篇 |
1967年 | 2825篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
71.
72.
Intraperitoneal stimulation of adoptively sensitized rats with bacterial antigen promotes the localization of lymphoblasts at the site of antigen deposition. Lymphoblast extravasation activity (LEA) is generated only when specifically immune donor lymphocytes and the recipients of these cells share at least on Ag-B haplotype. However, if the specificity criteria for its formation are satisfied, LEA promotes the local development of lymphoblasts of all available specificities and irrespective of their Ag-B genotype. Allogeneic lymphoblasts do not participate actively in the delayed inflammatory reaction even when they are passively recruited into exudates. The results suggest that LEA is a T cell-derived mediator that amplifies the delayed type hypersensitivity reaction by directing recently activated lymphocytes into lesions. 相似文献
73.
74.
Rosette and single-element strain gauges were implanted on the tibia in 2 dogs and recordings were made during locomotion on a treadmill. At foot contact and during the swing phase of locomotion, bone strains were low and directions of the principal strains were variable. There was a large shift in the directions of the principal strains at the beginning of the stance phase and bone strains were considerably higher. Peak strain occurred midway through the stance phase. At that time, the maximum principal strain (tension) was directed upwards and anteriorly between 30 and 60 degrees with respect to the long axis of the tibia. These bone strain patterns in the dog are similar to those found in sheep while both differ markedly from those found in humans. 相似文献
75.
76.
77.
To control the environmentally detrimental impact of acid rock drainage, two different countermeasures, layers of acid-buffering
materials and sodium dodecyl sulphate addition, were tested for their efficiency in laboratory percolation experiments. In
the experiment with a layer of calcium bentonite, only the iron output was reduced. The experiments with layers of concrete
grains demonstrated a decrease of the microbial activity as well as a precipitation of heavy-metal ions, whereas the cell
numbers did not decrease. Furthermore, finely grained concrete (1–5 mm) formed a water-tight hardpan (self-sealing layer).
In the experiment with 1 mM sodium dodecyl sulphate, all the microorganisms were killed and hence metal sulphide dissolution
was stopped. With 0.1 mM sodium dodecyl sulphate only a short, transient inhibition of leaching was achieved. The bacteria
remained alive.
Received: 16 February 1998 / Accepted: 23 February 1998 相似文献
78.
G. Franz M. Reindl S. C. Patel R. Beer I. Unterrichter T. Berger E. Schmutzhard W. Poewe & A. Kampfl 《Journal of neurochemistry》1999,73(4):1615-1625
Increasing evidence suggests that apolipoprotein D (apoD) could play a major role in mediating neuronal degeneration and regeneration in the CNS and the PNS. To investigate further the temporal pattern of apoD expression after experimental traumatic brain injury in the rat, male Sprague-Dawley rats were subjected to unilateral cortical impact injury. The animals were killed and examined for apoD mRNA and protein expression and for immunohistological analysis at intervals from 15 min to 14 days after injury. Increased apoD mRNA and protein levels were seen in the cortex and hippocampus ipsilateral to the injury site from 48 h to 14 days after the trauma. Immunohistological investigation demonstrated a differential pattern of apoD expression in the cortex and hippocampus, respectively: Increased apoD immunoreactivity in glial cells was detected from 2 to 3 days after the injury in cortex and hippocampus. In contrast, increased expression of apoD was seen in cortical and hippocampal neurons at later time points following impact injury. Concurrent histopathological examination using hematoxylin and eosin demonstrated dark, shrunken neurons in the cortex ipsilateral to the injury site. In contrast, no evidence of cell death was observed in the hippocampus ipsilateral to the injury site up to 14 days after the trauma. No evidence of increased apoD mRNA or protein expression or neuronal pathology by hematoxylin and eosin staining was detected in the contralateral cortex and hippocampus. Our results reveal induction of apoD expression in the cortex and hippocampus following traumatic brain injury in the rat. Our data also suggest that increased apoD expression may play an important role in cortical neuronal degeneration after brain injury in vivo. However, increased expression of apoD in the hippocampus may not necessarily be indicative of neuronal death. 相似文献
79.
Enhanced activation of a T cell line specific for acetylcholine receptor (AChR) by using anti-AChR monoclonal antibodies plus receptors 总被引:6,自引:0,他引:6
B C Schalke W E Klinkert H Wekerle D S Dwyer 《Journal of immunology (Baltimore, Md. : 1950)》1985,134(6):3643-3648
Immune complex-mediated regulation of the immune response has been studied by using T cell lines and monoclonal antibodies (MAb), both specific for the acetylcholine receptor (AChR). Rat T lymphocytes bearing the W3/25 phenotype and specific for AChR from Torpedo californica have been propagated in vitro for nearly 1 yr. These T cells proliferate in response to optimal concentrations of AChR presented by irradiated syngeneic thymus cells. At suboptimal concentrations of antigen there is little activation of the T cell line. We report here that the addition of small amounts of anti-AChR MAb produces dramatic stimulation of the T cell lines at suboptimal doses of AChR. Enhanced activation depends on the isotype and not the fine specificity of the MAb that are used. The observed phenomenon is antigen specific, and in fact, the immune complexes may actually suppress the proliferative response of irrelevant T cells to some extent. The MAb plus antigen are rapidly bound to the surface of the antigen-presenting cell, which we have shown is the dendritic cell. 相似文献
80.
Ultraviolet-induced restriction alleviation is an SOS function which partially relieves the K-12-specific DNA restriction in Escherichia coli. Restriction alleviation is determined by observing elevated survival of unmodified phage lambda in cells irradiated with ultraviolet prior to infection. We demonstrate that restriction of lambda is also relieved when log-phase cells are irradiated as late as 50 min after adsorption of lambda. At this time more than 60% of the lambda DNA is already released as acid-soluble material from the cells. Experiments involving reextraction of lambda DNA from infected cells and a mild detergent treatment removing absorbed phages from the cellular surface showed that only a small specific fraction of all lambda infections is destined to escape restriction due to restriction alleviation. This fraction (10-20%) has a retarded mode of DNA injection (60 min or longer) after adsorption which allows the expression of the restriction alleviation function before the phage DNA is exposed to restriction endonucleases. This behaviour of a fraction of lambda phages explains why the SOS function restriction alleviation could initially be discovered. We show that the retarded mode of DNA injection is not required for another SOS function acting on lambda DNA, the increased repair of ultraviolet-irradiated DNA (Weigle reactivation). 相似文献