Versuche zur Unterscheidung deutscher Winter- von Sommergetreidesorten und zur Entwicklungsbeschleunigung von Wintergersten

Theoretical and Applied Genetics -     

The determination of the red cell isoenzymes 6-phosphogluconate dehydrogenase (E.C.1.1.1.44) and acid phosphatase (E.C.3.1.3.2) by means of agarosegel thinlayer electrophoresis (author's transl)

Methods for the determination of the red cell isoenzymes 6-PGD and acP by means of agarosegel thinlayer electrophoresis are referred. Using these relatively simple techniques, results can be obtained after at least two hours. The quality of separation in both systems is higher than that obtained after starch gel electrophoresis.     

Bildungsabweichungen anGalanthus nivalis L.

Ohne Zusammenfassung     

Prolaktin

Ohne ZusammenfassungMit 17 Abbildungen.Aus dem Wissenschaftlichen Laboratorium der C. F. Boehringer & Soehne G. m. b. H., Mannheim-Waldhof.     

Transbilayer movement of 24Na in sonicated phosphatidylcholine vesicles exposed to frequency-modulated microwave radiation

Sonicated egg phosphatidylcholine vesicles loaded with ²⁴Na⁺ were exposed at 20mW to a frequency-modulated (3 Hz) microwave field in the range of 2350 to 2550 MHz, or at 80 mW to a 2450-MHz CW (continuous wave) field, in a waveguide. The vesicle suspension absorbed microwaves at about 1 mW/ml and 25 mW/ml (CW experiment). The average temperature change of the irradiated suspension was < 0.1 °C from ambient. Leakage of ²⁴Na⁺ from the vesicles for up to 19 hours was measured. No difference was noted in the movement of ²⁴Na⁺ from the vesicles in the irradiated and control dispersions.     

Biological Basis of Saki (Pithecia) Folk Species Recognized by the Matses Indians of Amazonian Perú

The Matses Indians of northeastern Perú recognize two linguistically labeled folk species of saki monkeys (Cebidae: Pithecia) that are said to be morphologically distinguishable, to prefer different habitats, and to be noninterbreeding. Because the systematic literature recognizes only one biological taxon of Pithecia in the area inhabited by the Matses, their folk taxonomy suggests either the presence of a previously undescribed species or a hitherto undocumented range extension. All known species of Pithecia are currently thought to be allopatric, so both possibilities are of interest. We obtained blood samples and anatomical voucher material from hunters' kills in order to test the biological basis for the Matses folk taxonomy of sakis. Molecular and morphological analyses of the collected material indicate that both Matses folk species correspond to one effectively panmictic population of Pithecia monachus. Overdifferentiation in folk classification systems, whereby one biological species is represented by two or more nonsynonymous folk species names, is a potentially widespread source of error that should be considered by researchers using local informants for primate field studies. In addition to resolving a folk-taxonomic enigma, our study provides the first quantitative analyses of local (within-population) morphological and molecular variation in this little-known platyrrhine genus.     

Subchronic toxicological investigations of Fusarium moniliforme-contaminated corn,culture material,and ammoniated culture material

The fungus Fusarium moniliforme is ubiquitous on corn throughout the world and is a likely co-contaminant on corn infested with aflatoxin-producing Aspergillus flavus. Ammoniation has been used to detoxify aflatoxin-contaminated commodities. To determine the effect of ammoniation on the toxic potential of Fusarium moniliforme, male Sprague-Dawley rats were fed either diets containing 10% sound corn, ammoniated corn, corn culture material of hepatotoxic F. moniliforme strain MRC 826 (CM), or ammoniated CM for four weeks. They were observed for signs of toxicity and hematological, serum chemical and histopathological evaluations were made. Groups of male Balb/c mice were fed diets fortified with 10% sound corn or CM for four weeks and evaluated by serum chemical and histopathological means to determine the suitability of mice as a model species for investigation of F. moniliforme-induced hepatotoxicity. Ammoniation was ineffective for detoxification of the CM. Hepatotoxicity and renal toxicity of CM and ammoniated CM were qualitatively similar, although renal tubular lesions appeared more advanced in rats fed ammoniated CM. Adrenal cortical cellular vacuolation was also found in CM and ammoniated CM-fed rats, while focal seminiferous tubular degeneration and aspermia were found only in the testes of ammoniated CM-fed rats. Fumonisin B₁ concentrations of the CM and ammoniated CM diets averaged 99 and 75 ppm, respectively. CM containing 99 ppm fumonisin B₁ also produced hepatotoxicity in mice similar to that found in CM-fed rats. Thus, mice may be useful for investigations of F. moniliforme-induced hepatotoxicity.Mention of a trademark, proprietary name or vendor does not imply its approval by the US Department of Agriculture to the exclusion of others that may also be suitable.