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排序方式: 共有237条查询结果,搜索用时 15 毫秒
41.
Lindström Irene Bontell Neil Hall Kevin E Ashelford JP Dubey Jon P Boyle Johan Lindh Judith E Smith 《Genome biology》2009,10(5):R53-17
Background
Toxoplasma gondii is a zoonotic parasite of global importance. In common with many protozoan parasites it has the capacity for sexual recombination, but current evidence suggests this is rarely employed. The global population structure is dominated by a small number of clonal genotypes, which exhibit biallelic variation and limited intralineage divergence. Little is known of the genotypes present in Africa despite the importance of AIDS-associated toxoplasmosis. 相似文献42.
43.
On association in a copula with time transformations 总被引:2,自引:0,他引:2
44.
J P Vendrell H Rabesandratana Y Jullien M F Huguet A Serre A Cannat 《Comptes rendus des séances de la Société de biologie et de ses filiales》1985,179(5):656-662
Two "protective" monoclonal anti-Brucella antibodies are described (SF311-IgG2a and S480-IgA) both of them accelerate the blood clearance of i.v. inoculated Brucella suis 1330 and decrease murine splenic infection on day 7. 相似文献
45.
Preparative isolation of the two forms of pig pancreatic pro-(carboxypeptidase A) and their monomeric carboxypeptidases A. 总被引:3,自引:1,他引:2 下载免费PDF全文
M Vilanova J Vendrell M T López C M Cuchillo F X Avilés 《The Biochemical journal》1985,229(3):605-609
A method is reported for the preparative isolation of the two forms of pro-(carboxypeptidase A) from pig pancreas: the monomer and the binary complex with pro-(proteinase E). This method, which is mainly based on chromatography on DEAE-Sepharose at pH 5.7, allows these proenzymes to be prepared more quickly and in safer conditions than with other reported methods. Undegraded and homogeneous carboxypeptidase A1 and A2 species (peptidyl-L-amino acid hydrolase, EC 3.4.17.1), in monomeric forms with high specific activity, are also obtained in high yield by controlled trypsin activation of either of the pro-(carboxypeptidases A) followed by chromatography on DEAE-Sepharose at pH 5.8 under dissociating conditions (7 M-urea). 相似文献
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Differential scanning calorimetric study of carboxypeptidase B, procarboxypeptidase B and its globular activation domain 总被引:1,自引:0,他引:1
F Conejero-Lara J M Sánchez-Ruiz P L Mateo F J Burgos J Vendrell F X Avilés 《European journal of biochemistry》1991,200(3):663-670
High-sensitivity differential scanning calorimetry has been applied to the study of porcine pancreatic carboxypeptidase B, the proenzyme and its 81-residue activation domain. The thermal study has been carried out over a range of scan rates, ionic strengths and pH values. The thermal unfolding of the isolated activation domain has been found to be reversible and corresponds to that of a typical compact globular structure, with melting temperatures higher than those of the enzyme and proenzyme. Both proteins, on the other hand, undergo an irreversible, highly scan-rate-dependent thermal denaturation under all the experimental conditions investigated. The denaturation of the enzyme at pH 7.5 and the proenzyme at pH 7.5 and 9.0 follows the two-state irreversible model [Sánchez-Ruiz, J.M., López-Lacomba, J.L., Cortijo, M. & Mateo, P.L. (1988) Biochemistry 27, 1648-1652]. Thus the kinetic constants and activation parameters of the denaturation process could be obtained and compared to those for other proteins, particularly those of the closely related carboxypeptidase A system. 相似文献
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In the course of a freeze-cleave study on intercellular junctions in the regenerating rat liver, we observed an unusual array of intramembranous particles located in regions of contact between endothelial cells lining the hepatic sinusoids. These arrays were characterized by an accumulation of particles which resembled a zonula occludens in their linear deployment but differed in that the contact regions were composed of individual particles which remained separated from each other by regular particle-free intervals. 相似文献