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61.
N Matheson I Bathurst J Travis 《Biochemical and biophysical research communications》1989,159(1):271-277
The replacement of ser359 with ala359 at the P1 position in human alpha-1-proteinase inhibitor results in the production of a variant protein containing 15% of the inhibitory activity of the normal inhibitor. Separation of active from inactive inhibitor on anhydrochymotrypsin-sepharose yields a form which has a second order association rate with neutrophil elastase which is approximately one half that for the native protein. These data indicate that the P1 residue is not of primary importance during the interaction of proteinases with alpha-1-proteinase inhibitor. Since substitution of alanine for serine causes the formation, primarily, of inactive inhibitor the major function of ser359 probably involves proper folding to give a functionally active inhibitory conformation. 相似文献
62.
J A Cohn N C Dougherty W F King 《Biochemical and biophysical research communications》1989,165(2):810-816
Protein phosphorylation responses in intact enterocytes were examined by stimulating 32Pi-labeled T84 cell monolayers with histamine and resolving proteins by two-dimensional gel electrophoresis. Histamine increases 32P-incorporation into two acidic proteins of Mr 83,000 and of Mr 29,000, designated p83 and p29. Labeling of p83 and p29 is also increased in cells exposed to ionomycin, but not in cells exposed to vasoactive intestinal peptide under conditions resulting in cAMP-mediated secretion and cAMP-stimulated protein phosphorylation. When T84 cell fractions are incubated with [gamma-32P]ATP, labeling of p83 is stimulated by Ca++, but not by cAMP. Thus, histamine stimulates Ca++-mediated protein phosphorylation during the regulation of Cl- secretion. 相似文献
63.
Liver mitochondrial respiratory functions decline with age 总被引:14,自引:0,他引:14
T C Yen Y S Chen K L King S H Yeh Y H Wei 《Biochemical and biophysical research communications》1989,165(3):944-1003
Human liver mitochondrial respiration rates in Chinese populations of various ages were assayed with an oxygraph. In this study, State 3 and State 4 respiration rates, respiratory control ratio (RCR), and ADP/O ratio were measured for 35 Chinese subjects of 31 to 76 years old. We found a significant negative correlation between age and respiratory control and ADP/O ratios tested. Moreover, the respiratory control and ADP/O ratios decreased with the increase of age. These findings suggest that a substantial fall in mitochondrial oxidative capacity in ageing liver may be an important contributor to the ageing process. 相似文献
64.
X-ray absorption spectroscopic (XAS) studies on cytochrome C1 from beef heart mitochondria were conducted to identify the effect of the hinge protein [Kim, C.H., & King, T.E. (1983) J. Biol. Chem. 258, 13543-13551] on the structure of the heme site in cytochrome c1. A comparison of XAS data of highly purified "one-band" and "two-band" cytochrome c1 [Kim, C.H., & King, T.E. (1987) Biochemistry 26, 1955-1961] demonstrates that the hinge protein exerts a rather pronounced effect on the heme environment of the cytochrome c1: a conformational change occurs within a radius of approximately 5 A from the heme iron in cytochrome c1 when the hinge protein is bound to cytochrome c1. This result may be correlated with the previous observations that the structure and reactivity of cytochrome c1 are affected by the hinge protein [Kim, C.H., & King, T.E. (1987) Biochemistry 26, 1955-1961; Kim, C.H., Balny, C., & King, T.E. (1987) J. Biol. Chem. 262, 8103-8108]. 相似文献
65.
Purified Staphylococcus aureus metalloproteinase contains trace amounts of a serine proteinase which rapidly degrades the metalloproteinase when EDTA is present. However, no degradation occurs when Ca2+ is added or if the serine proteinase is removed by immunoaffinity chromatography. Selective chelation of Zn2+ by o-phenanthroline, which reversibly inactivates the metalloproteinase, does not result in the degradation of the apometalloproteinase, even with excess of serine proteinase. These data are interpreted as follows: EDTA chelates enzyme-bound Ca2+ and Zn2+, causing irreversible inactivation as well as a conformational change in the metal-free protein. This allows proteolysis by the contaminating serine proteinase and explains why the metalloproteinase purified from serine proteinase-deficient strains of S. aureus was previously thought to be stable to autolysis. 相似文献
66.
A combination of quantum mechanical calculations and molecular dynamics simulations has been used to calculate the tautomer ratio of 4-(5-)methyl imidazole in solution, and the results are in good agreement with experiment. 相似文献
67.
Methods are presented to extract and purify mitochondrial DNA from the rotifer Brachionus plicatilis. The mtDNA obtained is of sufficient purity for digestion with restriction endonucleases. EcoR I restriction patterns are presented for 4 geographically separated clones. A restriction map based on digestion with 5 different restriction enzymes is included for one of these clones. Finally, use of mtDNA analysis for studies on the population structure and biogeography of rotifers is discussed. 相似文献
68.
Strain and sex differences in the response of mice to drugs that induce protoporphyria: role of porphyrin biosynthesis and removal 总被引:1,自引:0,他引:1
A hepatic green pigment, inhibitory toward ferrochelatase, has been isolated from the liver of mice treated with griseofulvin, isogriseofulvin, or 3,5-diethoxycarbonyl-1,4-dihydrocollidine and has been shown to exhibit identical chromatographic characteristics to authentic N-methyl protoporphyrin. All four possible structural isomers have been demonstrated, and each drug produced primarily the same isomer. N-Methyl protoporphyrin has also been found in very small amounts in the liver of untreated mice, but the isomeric composition appeared to differ from that of the drug-induced N-methyl protoporphyrin. Intraperitoneal administration of 3,5-diethoxy-carbonyl-1,4-dihydrocollidine to female C3H/He/Ola and NIH/Ola inbred mice produced a marked dose-related loss of hepatic ferrochelatase activity, which was identical in magnitude in the two strains. Induction of hepatic 5-aminolevulinate synthase (ALA-S), and accumulation of liver protoporphyrin, however, were greater in C3H/He/Ola mice. The strain difference in ALA-S response was most marked when inhibition of ferrochelatase (the "specific" effect of the drug) was maximal, and this suggests that a genetic variation exists in the sensitivity of ALA-S to a second drug action, the so-called nonspecific action, which is shared by many lipid-soluble compounds. Male mice of three strains accumulated greater amounts of hepatic protoporphyrin than females after treatment with griseofulvin, yet no significant difference was found between the two sexes in the extent of ferrochelatase inhibition. Stimulation of ALA-S activity was slightly greater in males, but when porphyria was very marked, ALA-S activities were significantly lower in this sex.(ABSTRACT TRUNCATED AT 250 WORDS) 相似文献
69.
70.
In the spring of 1986, 506 beef cows were used to evaluate the effectiveness of two estrus synchronization systems. Cows were synchronized with either a 6-mg Norgestomet implant placed in the ear for 14 d followed by a 6-mg Alfaprostol injection given 16 d after implant removal (Norgestomet-Alfaprostol) or with Syncro-Mate B (6-mg Norgestomet implant for 9 d with an injection containing 5 mg estradiol valerate and 3 mg Norgestomet at the time of implantation). The Alfaprostol injection in the Norgestomet-Alfaprostol group was given the same day as implant removal in the Syncro-Mate B group. These treatment groups were compared to a group of untreated controls. Cows were allotted to treatments by days postpartum, age and breed. Syncro-Mate B cows had a higher estrous response within 5 d after treatment (78.6 vs 64.0%) and a shorter interval to estrus (39.2 vs 66.7 h) than did Norgestomet-Alfaprostol cows (P < 0.05). Controls had a significantly lower estrous response compared to either of the synchronized groups (27.1%). The degree of estrus synchrony was identical in both synchronization systems (72.7%). Synchronized conception rate tended to be higher (P = 0.06) in the Norgestomet-Alfaprostol cows than in the Syncro-Mate B cows (74.5 vs 62.5%). Synchronized, 21-d, 25-d and breeding season pregnancy rates were 51.2, 70.8, 76.8 and 92.9% for Norgestomet-Alfaprostol cows; 48.5, 63.0, 73.2 and 87.8% for Syncro-Mate B cows; and 15.6, 56.3, 61.3 and 86.9% for control cows. The four pregnancy rates were not different between the two synchronization treatments (P > 0.10). Controls had lower synchronized and 25-d pregnancy rates when compared to either of the synchronized groups (P < 0.05). Days postpartum had no effect on the reproductive performance of cows synchronized with Norgestomet-Alfaprostol. Our results indicate that the Norgestomet-Alfaprostol system is as effective as Syncro-Mate B in synchronizing estrus in beef cows. 相似文献