Effects of a single short exposure to blue light on cognitive performance

The aim of the present study is to explore the effects of a single short one-minute exposure to blue light on cognitive performance. For this purpose, 32 young adults (16 females, mean age 24.06 ± 1.88 years) took part in a within-subjects research design, under two conditions: blue light and no light. Under both conditions, they performed the lexical decision task (LDT) in order to assess the degree of automatic activation of semantic memory through an embedded semantic priming (reaction times to prime – reaction times to target), together with the Attention Network Test (ANT) to assess the efficiency of the alerting, executive and orienting networks. During the LDT, a significantly stronger semantic priming under the blue light condition compared to no light was observed, while during the ANT a significant difference in orienting network efficiency between conditions was observed. The present data appear to highlight that even a single short exposure to blue light has an effect on cognitive performance in young adults.     

A mathematical model applied to the fungal colony growth of Sclerotium rolfsii

Colonies of Sclerotium rolfsii growing on solid medium showed a linear increase in radius simultaneously with exponential changes in colonial weight. The mathematical model developed by Koch (Koch, A.L. 1975. J. Gen. Microbiol. 89, 209216) to describe similar kinetics of mycelial growth was applied. Most of the parameters defined in the mentioned model were directly measured or calculated, except the values of K (maximum mycelium carrying capacity) and S (initial mycelial density) which were fitted to the experimental data of growth at 30°C and 23°C. The ratios K/S at 30°C and at 23°C were 18.8 and 1200 respectively, being consistent with two-dimensional growth. The model was predictive only during the exponential increase of the colony weight.     

Pregnancies following long luteal phases in southern white rhinoceros (Ceratotherium simum simum)

All extant species in the Rhinocerotidae family are experiencing escalating threats in the wild, making self-sustaining captive populations essential genetic reservoirs for species survival. Assisted reproductive technologies (ARTs) will become increasingly important for achieving and maintaining ex situ population sustainability and genetic diversity. Previous reports have shown that a large proportion of captive southern white rhinoceros (SWR) females are irregularly cyclic or acyclic, and that cycling females display two different estrous cycle lengths of approximately 30 or 70 days. It has been suggested that the longer estrous cycle length is infertile or subfertile, as no term pregnancies have been observed following long cycles. Here we report the achievement of two pregnancies following long luteal phases, using ovulation induction and artificial insemination with either fresh or frozen-thawed semen. One female SWR conceived on the first insemination attempt and gave birth to a live offspring. A second female conceived twice in consecutive long cycles although the first embryo was resorbed by 33 days post-insemination. A pregnancy from this female's second insemination is ongoing with expected parturition in November 2019. Whether prolonged estrous cycles in SWR are subfertile or infertile in natural breeding situations remains unclear. However, our findings demonstrate that the application of ARTs following prolonged cycles can result the successful establishment of pregnancies in SWR. Therefore, with ARTs, female SWR otherwise considered nonreproductive due to long estrous cycles may still have the potential for representation and contribution to the ex situ population.     

Antiestrogen stimulated human endometrial cancer growth: laboratory and clinical considerations

The new antiestrogen toremifene (TOR) is currently on the market for the treatment of advanced breast cancer in postmenopausal women. TOR is known to exhibit a similar efficacy profile as tamoxifen (TAM) in the treatment of advanced breast cancer and there are studies to suggest that the beneficial side effects of TAM on bone and blood lipids are also achieved with TOR. However, the data concerning the action of TOR on the endometrium is sorely lacking. In light of the estrogenic effect of TAM on the uterus and the 2–3-fold increased incidence in endometrial carcinoma detected in patients receiving TAM therapy, it is imperative to investigate the effect of TOR on endometrial carcinoma. We compared the actions of TAM and TOR on the EnCa101 human endometrial tumor model and find that both antiestrogens have similar growth stimulatory effects. To investigate a potential mechanism of antiestrogen-stimulated endometrial tumor growth, we have examined known activators of the AP-1 signal transduction pathway, the protein kinase C (PKC) family of isozymes, in the EnCa101 human endometrial tumor model. We find that increased PKC isozyme expression correlates with hormone-independent breast cancer as well as antiestrogen-stimulated endometrial cancer.     

Metabolic utilization of human osteoblast cell line hFOB 1.19 under normoxic and hypoxic conditions: A phenotypic microarray analysis

Osteoblasts play an important role in bone regeneration and repair. The hypoxia condition in bone occurs when bone undergoes fracture, and this will trigger a series of biochemical and mechanical changes to enable bone repair. Hence, it is interesting to observe the metabolites and metabolism changes when osteoblasts are exposed to hypoxic condition. This study has looked into the response of human osteoblast hFOB 1.19 under normoxic and hypoxic conditions by observing the cell growth and utilization of metabolites via Phenotype MicroArrays™ under these two different oxygen concentrations. The cell growth of hFOB 1.19 under hypoxic condition showed better growth compared to hFOB 1.19 under normal condition. In this study, osteoblast used glycolysis as the main pathway to produce energy as hFOB 1.19 in both hypoxic and normoxic conditions showed cell growth in well containing dextrin, glycogen, maltotriose, D-maltose, D-glucose-6-phospate, D-glucose, D-mannose, D-Turanose, D-fructose-6-phosphate, D-galactose, uridine, adenosine, inosine and α-keto-glutaric acid. In hypoxia, the cells have utilized additional metabolites such as α-D-glucose-1-phosphate and D-fructose, indicating possible activation of glycogen synthesis and glycogenolysis to metabolize α-D-glucose-1-phosphate. Meanwhile, during normoxia, D-L-α-glycerol phosphate was used, and this implies that the osteoblast may use glycerol-3-phosphate shuttle and oxidative phosphorylation to metabolize glycerol-3-phosphate.     

Advantages of single step over step-by-step screening for sleep disorders

Two secondary analyses of previously collected data were carried out to detect the best screening strategy for primary insomnia (Study 1) and narcolepsy type 1 (Study 2) between two different approaches: (A) step-by-step (i.e. first step: subjective assessment through mini sleep questionnaire – MSQ; second step: objective assessment through actigraphic assessment only for those positive at the first step) and (B) single step (i.e. simultaneous subjective and objective assessment). Forty-six healthy controls (HC) and 46 primary insomnia patients (PI) were analyzed in Study 1; 40 HC and 40 narcolepsy type-1 patients (NT1) were analyzed in Study 2. All participants originally wore the Micro Motionlogger Watch actigraph (Ambulatory Monitoring, Inc., Ardlsey, NY) around the non-dominant wrist for seven consecutive days, and upon completing the actigraphic assessment filled in the MSQ. In Study 1, the single-step approach correctly identified 93.48% of PI, while only 50% of PI were correctly identified by the step-by-step approach. In Study 2, 100% of NT1 was correctly classified through the single-step approach, while 72.5% according to the step-by-step approach. The result of both studies highlights the higher appropriateness of single step (i.e. bi-dimensional assessment) compared to step-by-step approach to screen primary insomnia and narcolepsy type-1 patients.     

In vivo liver and lung targeting of adriamycin encapsulated in glutaraldehyde-treated murine erythrocytes

Treatment of adriamycin-loaded erythrocytes from B6D2F1 mice with 0.1% glutaraldehyde produced the following effects: a considerable decrease in the in vitro leakage of the unmodified drug and a selective liver (and, to a lesser extent, lung) uptake of the encapsulated drug (70% of the injected dose) compared to drug leakage from, and tissue distribution of, carrier erythrocytes not treated with glutaraldehyde. The liver vascular bed was not saturated by five daily intravenous injections of 20 microliters of glutaraldehyde-treated erythrocytes, which allows a total dosage of 200 micrograms of the drug (half the LD50 value) to be administered. No appreciable liver damage results from extensive and prolonged uptake of glutaraldehyde-treated carrier erythrocytes. Entrapment of adriamycin within erythrocytes along with glutaraldehyde treatment of the carrier cells seems to be a promising therapeutic strategy against liver (and lung) tumors.