全文获取类型
收费全文 | 4787篇 |
免费 | 398篇 |
国内免费 | 1篇 |
出版年
2021年 | 42篇 |
2020年 | 29篇 |
2019年 | 56篇 |
2018年 | 71篇 |
2017年 | 47篇 |
2016年 | 56篇 |
2015年 | 124篇 |
2014年 | 149篇 |
2013年 | 249篇 |
2012年 | 240篇 |
2011年 | 242篇 |
2010年 | 163篇 |
2009年 | 122篇 |
2008年 | 242篇 |
2007年 | 253篇 |
2006年 | 244篇 |
2005年 | 243篇 |
2004年 | 223篇 |
2003年 | 244篇 |
2002年 | 196篇 |
2001年 | 167篇 |
2000年 | 215篇 |
1999年 | 164篇 |
1998年 | 73篇 |
1997年 | 61篇 |
1996年 | 61篇 |
1995年 | 53篇 |
1994年 | 57篇 |
1993年 | 33篇 |
1992年 | 113篇 |
1991年 | 92篇 |
1990年 | 95篇 |
1989年 | 86篇 |
1988年 | 69篇 |
1987年 | 50篇 |
1986年 | 57篇 |
1985年 | 38篇 |
1984年 | 56篇 |
1983年 | 36篇 |
1982年 | 24篇 |
1981年 | 20篇 |
1980年 | 32篇 |
1979年 | 32篇 |
1978年 | 26篇 |
1977年 | 25篇 |
1976年 | 23篇 |
1975年 | 21篇 |
1974年 | 27篇 |
1973年 | 27篇 |
1972年 | 20篇 |
排序方式: 共有5186条查询结果,搜索用时 15 毫秒
991.
Dimeric dihydrodiol dehydrogenases from pig liver, monkey kidney, and rabbit lens were inhibited more potently by 4-hydroxyphenylketones such as 4-hydroxybenzaldehyde, 4-hydroxyphenylglyoxal, and 4-hydroxyacetophenone than by isoascorbate and ascorbate, known inhibitors of the enzymes. No significant inhibition was observed with 2- or 3-hydroxyphenylketones, phenylketones with a functional group other than a hydroxy group at the 4-position, and 4-hydroxyphenyl derivatives without a carbonyl group. The steady-state kinetic analyses of the inhibition of the pig liver enzyme indicated that the 4-hydroxyphenylketones, similarly to ascorbate and its epimer, bound to an enzyme-NADP+ binary complex as competitive inhibitors with respect to dihydrodiol substrate. The inhibition by the 4-hydroxyphenylketones was uncompetitive with respect to isoascorbate, and the addition of one of the 4-hydroxyphenylketones or isoascorbate with NADP+ afforded a great protective effect against inactivation of the enzyme by diethylpyrocarbonate or by heat treatment, which indicates that 4-hydroxyphenylketones and isoascorbate bind at the same site in or near the active center of the enzyme. The structural comparison of 4-hydroxybenzaldehyde and ascorbate suggests that the hydroxy group at C-5, carbonyl group at C-1 and lactone ring of ascorbate are important for the binding to the enzyme. 相似文献
992.
993.
994.
995.
996.
Shozo Fujioka Akira Sakurai Naotsugu Sugimoto Isomaro Yamaguchi Noboru Murofushi Nobutaka Takahashi 《Bioscience, biotechnology, and biochemistry》2013,77(8):2053-2059
Efforts were made to isolate flower-inhibitory substances from extracts of the short-day plant Lemna paucicostata 381. Lemna paucicostata 151, which was used in the bioassay, exhibits poor flowering in response to the photoperiod, but flowers profusely in response to benzoic acid. Therefore, only those substances that inhibit benzoic acid-induced flowering were studied. Several fractions obtained by silica gel column chromatography exhibited flower-inhibitory activity when tested on L. paucicostata 151. After several purification steps, one of the active principles was identified as lutein by MS, UV and NMR spectroscopic analyses. Lutein and its isomer zeaxanthin inhibited benzoic acid-induced flowering in both L. paucicostata 151 and 381. 相似文献
997.
Tsutomu Yamaguchi Yukiko Yamashita 《Bioscience, biotechnology, and biochemistry》2013,77(7):1675-1678
Three phytotoxins named pyrenochaetic acids A, B and C were isolated from culture filtrates of Pyrenochaeta terrestris, and the structures were determined to be 4-crotonoyl-3-methoxy-5-methylbenzoic acid (I), 4-(3-hydroxybutyroyl)-3-methoxy-5-methylbenzoic acid (II) and 4-butyroyl-3-methoxy-5-methylbenzoic acid (III), respectively. Their phytotoxicities were demonstrated in bioassays. 相似文献
998.
chlorpromazine interacted preferentially with membrane proteins rather than membrane lipids in the initial incorporation into human erythrocyte ghosts, as demonstrated by means of the fluorescence quenching and a maleimide spin label. In this state the membrane fluidity increased. At higher concentrations of chlorpromazine, the membrane fluidity decreased and a motionally restricted signal from fatty acid spin labels appeared predominantly. However, no such signal appeared in protein-free vesicles. The temperature and pH dependences of the outer hyperfine splitting of this restricted signal were very similar to those of bovine serum albumin. On the basis of sodium dodecyl sulfate-polyacrylamide gel electrophoresis of chlorpromazine-treated and -untreated ghosts, it was found that there was no significant difference in membrane proteins between both samples except for the changes of a few bands which were not directly concerned with the occurrence of this restricted signal. These results suggest that the fatty acid spin labels bind preferably to membrane proteins as the lipid domain becomes packed with chlorpromazine. 相似文献
999.
Purification and characterization of the flagellar hook-basal body complex of Salmonella typhimurium. 总被引:46,自引:33,他引:13 下载免费PDF全文
The hook-basal body complex of Salmonella typhimurium, a major component of its flagellar apparatus, was subjected to detailed analysis by electron microscopy and gel electrophoresis. The study was facilitated by the development of an improved protocol for isolation of the complexes in high yield and purity. Nine proteins were identified with the structure. These proteins had apparent molecular weights of 65,000 (65K), 60K, 42K, 38K, 32K, 30K, 27K, 16K, and 14K. Small but reproducible shifts in the apparent molecular weights of specific proteins from conditionally nonflagellate mutants indicated the following gene-polypeptide correspondences: flaFV, 42K; flaFVI, 32K; flaFVII, 30K; flaFIX, 38K; flaAII.1, 65K. Several new morphological features of hook-basal body complexes were recognized, including a clawlike structure on the cytoplasm-proximal M ring and additional material at the cytoplasmic face of the M ring. Based on this study and the work of others, we suggest that the morphological features of the hook-basal body complex correspond to the following proteins: hook-filament junction, 60K; hook, 42K; rod, 30K and 32K; L ring and outer cylinder wall, 27K; P ring, 38K; S ring, unknown; M ring 65K. 相似文献
1000.
T Yokota T Yamaguchi K Kitamura E Otsuji K Sawai T Takahashi 《The journal of histochemistry and cytochemistry》1990,38(4):585-588
We examined the tissue localization of biotin-labeled murine monoclonal antibody (MAb) S202 directed against the human scirrhous gastric carcinoma cell line MK-01 in normal and tumor-bearing mice after intravenous (IV) administration. The biotin-labeled MAb proved to be stable in vivo under normal conditions, antibody titer being 1:256 at 4 hr after IV injection. At 24 hr after injection, the tumor was stained by the avidin-biotin-peroxidase complex (ABC) method. Biotin-labeled MAb was found to be suitable for detection of the xenografted tumor of nude mice. This study provides new information concerning the dynamics of the distribution of biotin-labeled MAb in vivo. 相似文献